LOCUS       sequence-171233-       12835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 12835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Feb 15. 26(7):2000-9."
COMMENT     Sequence Label: pCI-SEP_NR2A
FEATURES             Location/Qualifiers
     source          1..12835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     561..580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        871..1250
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1251..1454
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1405..1425
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1451..1475
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1590..1722
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1705..1724
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1767..1785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1792..1808
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1793..1809
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2326..3036
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2353..2381)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2976..2996
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(10642..10658)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10642..10658)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    10682..10803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10693..10712)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10747..10766
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      10984..11439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11071..11090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11281..11302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11506..11525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11625..11647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11685..11703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11771..11875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11876..11944,11945..12736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12094..12113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      721 gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat atagcataaa
      781 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      841 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      901 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      961 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1021 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1081 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     1141 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     1201 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1261 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1321 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1381 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1441 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
     1501 atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct
     1561 gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
     1621 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
     1681 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
     1741 attacagctc ttaaggctag agtacttaat acgactcact ataggctagc ctcgaggtcg
     1801 acggtatcga taagcttgat atcgaattcc gttgctgtcg caggagcgct cagaggaccg
     1861 gcagtcgctg tccggagtgg aacagaaagc tgagccaggg ctctagaaga gaggctcctg
     1921 aggtgctgtg cctgagcatg gggctggatg aggtctgaga gtcgcggcgg cagcaatcag
     1981 ccctggagat gaccaggggt ggccactgct gagaactatg tggagagagg ctgcgagccc
     2041 tgctgcagag cctccggctg ggatagccgc cccccgtggg ggtgatgcgg acagcgcggg
     2101 acagccaggg gagcgcgcgg gggccgcagc atgcgggaac ccgctaaacc tggtggctgc
     2161 tgaggcggcc gagatgctcg tgcgcgcagc acgccccatt gcatcctcca ccttctccgg
     2221 ctacagggac cctaagtggc gaccatgggc agattgggct actggacctt gctggtattg
     2281 ccggcccttc tggtctggcg cgatccggcg cagaacgcgg ctaggagtaa aggagaagaa
     2341 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     2401 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     2461 atttgcacta ctggaaaact acctgttcct tggccaacac ttgtcactac tttaacttat
     2521 ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt tttcaagagt
     2581 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     2641 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2701 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2761 gatcaccagg tgtacatcat ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
     2821 attagacaca acattgaaga tggaggcgtt caactagcag accattatca acaaaatact
     2881 ccaattggcg atgggcccgt ccttttacca gacaaccatt acctgtttac aacttctact
     2941 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     3001 gctgggatta cacatggcat ggatgaacta tacaaagcta gcgcggcgga gaagggtcct
     3061 ccagcgctga acattgcggt gctgctgggt cacagccacg acgtgacaga acgcgaactt
     3121 cgaaatctgt ggggcccaga gcaggcaacc ggcttgcccc tggatgtgaa cgtggtggcg
     3181 ttattgatga accgcactga ccctaagagc ctcatcacgc atgtgtgcga cctcatgtcc
     3241 ggggcgcgca tccacggctt ggtgtttgga gatgacacgg accaggaggc tgtggcccag
     3301 atgctggatt ttatctcctc acagactttt atccccatct tgggcattca tgggggtgca
     3361 tctatgatca tggctgacaa ggatccgaca tccacgttct tccagtttgg agcctccatc
     3421 cagcagcaag ccacagttat gctgaagatc atgcaggact acgactggca cgtcttctcc
     3481 ctggtcacca ccatcttccc tggctaccga gacttcatca gctttatcaa gacaacagtg
     3541 gacaacagct ttgtgggctg ggatatgcag aacgtgatca cactggacac ctccttcgag
     3601 gacgccaaga cgcaggtcca gctgaagaag atccattctt ctgtcatcct gctctactgc
     3661 tccaaagacg aggctgtcct catcctgagc gaggctcgct ccttcggcct cactggctat
     3721 gatttcttct ggattgtccc cagtttggtg tctgggaaca cagagctcat ccccaaagag
     3781 tttccatcag gtctcatttc agtctcttat gacgactggg actacagcct ggaggcaaga
     3841 gtgagagacg gtcttgggat cttaaccact gccgcatcct ccatgttgga gaagttctcc
     3901 tacattcctg aggccaaggc cagctgctat gggcaggcag agaagccaga gaccccgcta
     3961 cacaccctgc accaattcat ggtcaatgtg acttgggatg gcaaggactt gtccttcact
     4021 gaggaaggtt accaggtgca ccccaggctt gtggtgatcg tgctgaacaa ggaccgggag
     4081 tgggaaaagg tgggcaagtg ggagaaccag acgctgagcc tgaggcacgc tgtgtggcca
     4141 aggtacaagt ccttttctga ctgcgagcca gatgacaacc acctcagcat tgtcaccttg
     4201 gaggaagccc ccttcgtcat cgtagaggac atagaccccc tgactgagac ctgtgtgagg
     4261 aacacggtgc cctgtcggaa gttcgtcaag atcaacaatt caaccaacga agggatgaat
     4321 gtgaagaaat gctgcaaggg gttctgcatc gacatcctca agaagctgtc cagaactgtg
     4381 aagttcacct atgacctcta cctggtgacc aatgggaagc atgggaaaaa ggttaacaat
     4441 gtgtggaatg gaatgatagg tgaagtggtc tatcaacgag cagtcatggc tgtgggctca
     4501 ctcaccatca atgaggagcg ttcggaagtg gtggacttct cggtgccctt tgtggagaca
     4561 ggaatcagcg tcatggtctc caggagtaat ggcactgtct ccccttctgc tttcctcgaa
     4621 cccttcagtg cctccgtctg ggtgatgatg tttgtgatgc tgctcatcgt ctcagccatt
     4681 gctgtcttcg tttttgaata cttcagtcct gttggataca acagaaactt agccaaaggg
     4741 aaagctcccc acgggccttc ttttactatt ggaaaagcta tatggctcct ctggggcctg
     4801 gtcttcaaca attctgtgcc tgtccagaat cctaaaggca caaccagcaa gatcatggtg
     4861 tcagtgtggg ccttctttgc tgtcatcttc ctggccagtt acacagccaa cctggctgcc
     4921 ttcatgatcc aggaggagtt tgtggaccaa gtgactggcc tcagtgacaa gaagttccag
     4981 agacctcatg actattctcc acctttccga tttgggacgg tacccaatgg aagtacagag
     5041 aggaatattc gtaacaacta cccgtatatg caccagtaca tgaccagatt caaccagagg
     5101 ggagtggagg atgccttggt cagcttgaaa accgggaagt tggacgcttt catctatgac
     5161 gcagccgtct tgaactacaa ggccgggagg gatgaaggct gtaaactggt gaccattggg
     5221 agcgggtaca tctttgctac cacaggctat ggaattgcgc tgcagaaggg ctcaccctgg
     5281 aagaggcaga ttgacctcgc tctgctccag tttgttggtg atggtgagat ggaggagctg
     5341 gagacactgt ggcttacggg catctgccac aacgagaaga atgaggtgat gagtagccag
     5401 ctggacatcg ataacatggc gggcgtgttc tacatgctgg ctgcagccat ggccctcagc
     5461 ctcatcacct tcatctggga gcacctcttc tactggaagc tgcgcttctg cttcacaggc
     5521 gtgtgctctg accggcccgg gctgctcttc tccatcagca ggggcatcta tagttgcatc
     5581 catggggtac acattgaaga aaagaagaaa tctccagatt tcaatctgac tggatcacag
     5641 agcaacatgc taaagcttct tcggtcagct aaaaacatct ccaatatgtc caacatgaac
     5701 tcctcaagaa tggactcacc taaaagagct actgacttca ttcaaagagg gtcacttatt
     5761 gtggacatgg tttcagacaa gggaaatttg atatattcag acaacagatc ctttcaaggg
     5821 aaggacagta tatttggaga caacatgaat gaactccaaa catttgtggc caacaggcac
     5881 aaggataatc tcagtaacta tgtgtttcaa ggacagcatc ctctcactct caatgagtcc
     5941 aaccctaaca cagtagaggt ggctgtcagc actgaatcca aagggaactc ccgaccccgg
     6001 cagctttgga agaaatccat ggagtctcta cgccaggatt ctctaaacca gaacccagtc
     6061 tcccagaggg atgagaagac tgcagagaat cggacccact cgctaaagag tcctaggtat
     6121 cttccagaag aggtagccca ctctgacatt tcagaaacct caagccgggc cacatgccac
     6181 agggagccag ataacaataa gaaccacaag accaaggata acttcaaacg gtcaatggcc
     6241 tctaagtatc ccaaggactg tagcgatgtt gaccgcacct acatgaaaac caaagcaagt
     6301 tctcccaggg ataagatcta taccattgat ggtgagaagg agcccagctt ccacttagat
     6361 cctcctcagt ttgttgagaa tataaccctg cctgagaatg tgggcttccc agatacctac
     6421 caagatcaca atgagaactt ccgcaagggg gactccacac tgcccatgaa caggaaccca
     6481 ttacataatg aagacgggct tcccaacaat gaccaatata aactctatgc caagcacttt
     6541 accttgaaag acaagggttc cccacacagt gagggcagtg atcgataccg gcagaactcc
     6601 acacattgca gaagctgcct ttcgaatctg cccacctact caggccactt taccatgagg
     6661 tctcctttca agtgtgatgc ctgtctgcgg atggggaatc tctatgacat tgatgaagac
     6721 cagatgcttc aggagacagg taacccagct actcgggagg aggtctacca gcaggactgg
     6781 tcacagaaca acgccctcca gttccagaag aacaagctaa ggattaaccg acagcactcc
     6841 tatgataaca ttctggacaa acccagagag atagacctta gcaggccctc ccggagcata
     6901 agcctcaagg acagggaacg gctactggag ggcaacttgt atgggagcct gttcagtgtc
     6961 ccctcaagca aactcttggg gaacaaaagc tcccttttcc cccaaggtct ggaggacagc
     7021 aagaggagca agtctctctt gccagaccac gcctccgata atcctttcct ccacacgtat
     7081 ggggatgacc aacgcttagt tatcgggaga tgtccctcgg acccttacaa acactcattg
     7141 ccatcacagg cggtaaatga cagctatctt cggtcatcct tgaggtcaac agcatcatat
     7201 tgctccaggg acagtcgggg ccacagtgat gtgtatattt cagagcatgt tatgccttat
     7261 gctgcaaata agaataccat gtactctacc cccagggttt taaattcctg cagcaataga
     7321 cgagtgtaca agaaaatgcc tagtatcgaa tctgatgttt aagatcttcc atcagtattt
     7381 atctataagg aaacatatag aatggccaac attatagagg gtaaatgttg gatgtccgat
     7441 agcaccctac taggaggaag agggtacagg gaggtacttt ttgttggctc ttttgcacat
     7501 ggctccatgc cataatcttc cactcaagga atcttgtgag atatgtgctg agcacagcat
     7561 ataccacgta ggtgaatcct taaccaaaaa caaataaata cacatgggca agtctcccag
     7621 acatggcgac tgggcacggc ggcaataatg gtgcatcaga cggcgatggt gacattgtgg
     7681 ttgcctatat tccaaattcc atgaagatca atccaccatg taacttcccc catcagaaat
     7741 gcctcacagt ttctctaata cagaataagc aatatggtat gcatgtaagt ctgacgcaga
     7801 caataagaca gttaagaatg catctgcact gtagtaagat tgacatgtgc aaggattaag
     7861 aagtttggct tgtaacagtt ctcagatttc ttgttatgcc ttccatgaca accctggatc
     7921 agtccccctt aaactccagg ttcgcccccc cccccaaaga atagcaaatc agcgtgtttg
     7981 tggcacccac actactttca ttgtaggctt cccccaagac acacctggaa caaatagccc
     8041 cagggactct caggtggaga actgtaagaa tctctttgga tgttaatgtg tgtgtatctc
     8101 tcaccctcag cttcattggt cttgttcgga gctgcataaa cacttttatg tcttcgatat
     8161 ctaagtgtgg atcttctgtc tatgacataa tggctgttgt agttaatctt gaagactcat
     8221 gtacgtaagt ctgcatttcc tcccttctgg tgatgactca gggttgtata gtatctctta
     8281 cctgacccct cccagagtaa cgtaacttgt tctgtcccca gacagccatg atggtgtcta
     8341 actagctgtg tgttgtgttc ctagagttgt taatgtggtg acatgtgcca gcagccatgt
     8401 gtaatctgta agaagtccca tgctatctgt gtgtccaagg ctagcatggt tttacattta
     8461 tgtccctcaa agtcaggaaa ctcttagaca attatttcta tacaaacctc ctcaaatcaa
     8521 aagccttcag acatcagcca cttgtgtgtc cccccccaag cacccttcca actgtggata
     8581 aactcagtaa aacaagggat ttctatcctc tgaaatgtac accttcttgg aggtgaattt
     8641 cttccccagc accaacagaa accaatgaga agtcttcaac gcatttgtca tttctgagct
     8701 gcctgcattg tcctattctt ggcccactga gcctgtgcat atatgacaga acagatactc
     8761 attagattaa aaaaaagaaa aactggagcc aatgagggga atattcgtgc atggaaaatg
     8821 tcttagcaca taatttttta tcacattttt aaaaaggact caaaaggtag ttatgatacc
     8881 ttattagaca aggacattca caaaagcaga aaataatatg aattaaacat tttatatgct
     8941 tcttttctag caattagtat atttccaatg cattattgtc cttgaaatag ccaggctgct
     9001 gaccgtggta tttcgcagga gactttagtt ggttgggtaa ctctctccca tgaaggattc
     9061 taaccatttc ttgtttttcc tcttactgaa tcttggcttg ggttcttgga gaaagggatc
     9121 gcacgacagg ttgaaatttc atccctgttc ttttcttcgt ggtgttctat tttataggtg
     9181 ggattgtcca actacaagtt aagcagggaa ggttctctta gagcttgggg ctgcttcaga
     9241 aggagctagc agagggaaag ttcatttgac ctacagtgtt gcagaatgac aaaaagtgat
     9301 gcaatacacc ggaggaccag aggtggagtg gcagaggggg tgaggatctc tcttcctgtg
     9361 ctcttgcaga acacagaagg aggctggggt ggagctagag ggctgaggtc acaggactag
     9421 ggtggagcca gcggtctggg gcggagctag aaggctgagg tcacaggact agggtggagc
     9481 cagtggtctg gggcggagct agaaggctgg ggcggagcta gaagttgata agctttaaag
     9541 tcacgtttta ttccttggac agagccatcc tttccttccc caagtcccac agctggggaa
     9601 atgtgcatta gagaacctag aacagtctcc ctgatttttg atcttaaatt tgagcttcag
     9661 tgtgtgtagt gtcctttcac cccttttact ttatttcccc ataggattcc acacctgtat
     9721 tcctcctgct aggactgctg ttgtgtcctc acagaactga ctcttaagcc tccctggaat
     9781 acagaatcat gacttgtcac aaatctgagg agcattctgc tttcccggat tgtcctcaaa
     9841 gcccagggtg ctgttgaatg tcccacattt tagtgagagt acaaatggca tttatactgc
     9901 ctcctcaaaa ccctctctgg aaaattcagg atcatttctg ctattaactc tgtacaaaag
     9961 aagctaaaat gaggcttttg gaagaatcag tcacataatg ggaaagaata cgtttgtttt
    10021 aacaccaaga tatttacaaa aataagtcat ggtagttagg ggctgtgtgg caaacatttg
    10081 cttttccctc tcctctaatt tgctgcctct tgcacatgat aacttcaaag aagaaaacga
    10141 ggaggtcacg ttccaagtga gaggtaatca tgtgggcttg tttgaaggaa agccaagtga
    10201 gatccatcct tttcaaaact aaccccgagg gtggcaaatg gcacctacgg ccggtgatga
    10261 gtactgagca agaggccttg gcctgggtat cacttagagg gttcccacac taaacccaac
    10321 ctctgagtcc ttagacttac agatgctaag aagccagtgt ctcttaactc aaaggcctga
    10381 tctttccctg gactaagtca gggcagctgc cctgggcagt tgtcttggca attcttttgt
    10441 tttcctcatt ttggaaagga ccagactggt cctcagatgt caatacttga aagcaaacag
    10501 ccatgcatct gtccttgcac ctgaaccggt gcaaatgctg tgcacaagag aagctcccag
    10561 agcatgacct tcactcggcc gcgcgacagc aacggaattc cgttgctgtc gcgacagcaa
    10621 cggaattcct gcagcccggg ggatccacta gttctagagc ggccgcttcg agcagacatg
    10681 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
    10741 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
    10801 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
    10861 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt
    10921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10981 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    11041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    11101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    11161 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    11221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    11281 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    11341 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    11401 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
    11461 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
    11521 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    11581 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    11641 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
    11701 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    11761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    11881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    11941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    12121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    12241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    12301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    12541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    12601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    12661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    12721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    12781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatg
//
