LOCUS       sequence-171234-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UPRE-mCh-Ubc-Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE,
            Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit 
            A, Parnas O, Regev A, Weissman JS
  TITLE     A Multiplexed Single-Cell CRISPR Screening Platform Enables 
            Systematic Dissection of the Unfolded Protein Response.
  JOURNAL   Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 
            10.1016/j.cell.2016.11.048.
  PUBMED    27984733
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1867-1882.e21"
COMMENT     Sequence Label: pBA407
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    244..361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(654..698)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    883..1116
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1613..1738
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1785..2418
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2610..2629)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2679..2695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2679..2695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2692..2714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2703..2719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2727..2733,2734..2751,2752..2757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2772..2793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3033..3050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3204..3792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3284..3303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3963..4754,4755..4823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4586..4605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4824..4928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4996..5014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5133..5366
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5441..6029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5547..5558
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5956..5976
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6206..7000)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6206..7000)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6317..6336)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6927..6946
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7046..8257)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7190..7209)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(8343..8369)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(8401..9111)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8679..8698)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8948..8966
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgaggaatcg ccacgtcagc acctgtctcg aggaatcgcc acgtcagcac ctgtctcgag
       61 gaatcgccac gtcagcacct gtctcgagga atcgccacgt cagcacctgt ctcgaggaat
      121 cgccacgtca gcacctgtct cgagcaagct tgggctgcag gtcgagcccg ggctagcacg
      181 cgtaagagct cggtaccgga tcctagtcca aactggatct ctgctgtccc tgtaataaac
      241 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
      301 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
      361 attgtggatg aatactgcca tttgtgaatt cggcgatacc gtcgagatcc gttcactaat
      421 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
      481 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
      541 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
      601 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
      661 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
      721 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
      781 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
      841 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
      901 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
      961 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     1021 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     1081 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     1141 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     1201 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     1261 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     1321 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     1381 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     1441 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     1501 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     1561 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     1621 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     1681 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     1741 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     1801 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     1861 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     1921 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag
     1981 cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc
     2041 tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga
     2101 agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc
     2161 actctaacac ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
     2221 tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt
     2281 agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat
     2341 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg
     2401 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
     2461 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
     2521 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
     2581 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2641 agtgaaaaaa atgctttatt tgtgaaattc gtaatcatgt catagctgtt tcctgtgtga
     2701 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2761 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2821 cagtcgggaa acctgtcgtg ccacggggag ctttttgcaa aagcctaggc ctccaaaaaa
     2881 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     2941 aaaaaaaatt agtcagccat gagcttgctg cattaatgaa tcggccaacg cgcggggaga
     3001 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4561 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4921 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4981 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atgatctagc
     5041 cctttcctta attaacccgg gcctctcact ctctgatatt catttctttg caagttataa
     5101 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     5161 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     5221 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     5281 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     5341 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     5401 tacagctgcc ttgtaagtca ttggtcttaa aggatctcag gcggggaggc ggcccaaagg
     5461 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     5521 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     5581 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     5641 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     5701 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     5761 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     5821 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     5881 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     5941 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     6001 tcacaaattt tgtaatccag aggttgatta ggatctatcg cgataagctt gatatcgaat
     6061 tgggagggac taaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6121 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta
     6181 gagtcgcggc cgctttactt gtacatcaga agaactcgtc aagaaggcga tagaaggcga
     6241 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     6301 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6361 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6421 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     6481 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6541 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6601 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6661 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     6721 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     6781 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     6841 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     6901 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     6961 cggccggaga acctgcgtgc aatccatctt gttcaatcat ggtggaccgg tacggggtgg
     7021 agatccgagc tcggtaccaa gcttcgtcta acaaaaaagc caaaaacggc cagaatttag
     7081 cggacaattt actagtctaa cactgaaaat tacatattga cccaaatgat tacatttcaa
     7141 aaggtgccta aaaaacttca caaaacacac tcgccaaccc cgagcgcata gttcaaaacc
     7201 ggagcttcag ctacttaaga agataggtac ataaaaccga ccaaagaaac tgacgcctca
     7261 cttatccctc ccctcaccag aggtccggcg cctgtcgatt caggagagcc taccctaggc
     7321 ccgaaccctg cgtcctgcga cggagaaaag cctaccgcac acctaccggc aggtggcccc
     7381 accctgcatt ataagccaac agaacgggtg acgtcacgac acgacgaggg cgcgcgctcc
     7441 caaaggtacg ggtgcactgc ccaacggcac cgccataact gccgcccccg caacagacga
     7501 caaaccgagt tctccagtca gtgacaaact tcacgtcagg gtccccagat ggtgccccag
     7561 cccatctcac ccgaataaga gctttcccgc attagcgaag gcctcaagac cttgggttct
     7621 tgccgcccac catgcccccc accttgtttc aacgacctca cagcccgcct cacaagcgtc
     7681 ttccattcaa gactcgggaa cagccgccat tttgctgcgc tccccccaac ccccagttca
     7741 gggcaacctt gctcgcggac ccagactaca gcccttggcg gtctctccac acgcttccgt
     7801 cccaccgagc ggcccggcgg ccacgaaagc cccggccagc ccagcagccc gctactcacc
     7861 aagtgacgat cacagcgatc cacaaacaag aaccgcgacc caaatcccgg ctgcgacgga
     7921 actagctgtg ccacacccgg cgcgtcctta tataatcatc ggcgttcacc gccccacgga
     7981 gatccctccg cagaatcgcc gagaagggac tacttttcct cgcctgttcc gctctctgga
     8041 aagaaaacca gtgccctaga gtcacccaag tcccgtccta aaatgtcctt ctgctgatac
     8101 tggggttcta aggccgagtc ttatgagcag cgggccgctg tcctgagcgt ccgggcggaa
     8161 ggatcaggac gctcgctgcg cccttcgtct gacgtggcag cgctcgccgt gaggaggggg
     8221 gcgcccgcgg gaggcgccaa aacccggcgc ggaggccaga tcttgggtgg gttactccag
     8281 actgccttgg gaaaagcgcc tcccctaccc ggtagaattt ctagtttaat taatcattac
     8341 taagcgtagt ctgggacgtc gtatgggtat tcgaaccgcg ggccctctag actcgaggtt
     8401 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
     8461 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
     8521 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
     8581 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     8641 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     8701 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     8761 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     8821 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     8881 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     8941 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     9001 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     9061 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tgttaacgga
     9121 gatcctctag agtcgactct agaggccgcc ggctcagtct tggcttctca gttgctcgct
     9181 gcagatgcgg ttggagtacg aggcgccgca gccactgctt ttataacaag cgttttatga
     9241 atgagtgcag atct
//
