LOCUS       sequence-171239-        7319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Lee E, De Camilli P
  TITLE     Dynamin at actin tails.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Jan 8. 99(1):161-6.
  PUBMED    11782545
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Jan 8. 99(1):161-6."
COMMENT     Sequence Label: pEGFPN1-human Dynamin 1aa
FEATURES             Location/Qualifiers
     source          1..7319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      261..270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(267..269,270..272,273..986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(267..269,270..272,273..983)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(312..333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(573..592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     920..941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1109..1230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1146..1165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1200..1219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1237..1692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1374..1395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1586..1605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1719..1823
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1825..2182
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1825..2016
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1848..1868)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2033..2168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2217..3011
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2217..3011
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2271..2290)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2881..2900
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3199..3218)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3243..3290
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3619..4207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4108..4127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4382..4685
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4686..4889
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4840..4860
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtcgcgggg ccctgctcct gggcctccgc ctgctgggtc cgccctgggg ggggcgcccc
       61 ccgtgccctc caggccgggg gcttcccctg accctttcgg ccctccccct caggtgccct
      121 cgcgccccaa ccgcgccccg cccggggtcc ccagccgatc gggtcaggca agtccatccc
      181 gtcctgagag ccccaggccc cccttcgaca agcttcgaat tctgcagtcg acggtaccgc
      241 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      961 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     1021 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1081 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1201 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1261 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1321 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1381 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1441 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1501 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1561 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1621 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1681 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1801 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     1861 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1921 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1981 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2101 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2161 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2221 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2281 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2341 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     2401 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2461 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2521 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2581 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2641 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     2701 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     2761 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     2821 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     2881 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     2941 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3001 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3061 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3121 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3181 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3241 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3301 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     3361 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     3421 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     3481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3721 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4261 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     4321 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4381 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4441 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4501 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4561 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4621 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4681 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4741 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4801 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4861 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
     4921 accggactca gatcctgcca tgggcaaccg cggcatggaa gatctcatcc cgctggtcaa
     4981 ccggctgcaa gacgccttct ctgccatcgg ccagaacgcg gacctcgacc tgccgcagat
     5041 cgctgtggtg ggcggccaga gcgccggcaa gagctcggtg ctcgagaatt tcgtaggcag
     5101 ggacttcttg cctcgaggat ctggcattgt tacccgacgt cccctggtct tgcagctggt
     5161 caatgcaacc acagaatatg ccgagttcct gcactgcaag ggaaagaaat tcaccgactt
     5221 cgaggaggtg cgccttgaga tcgaggccga gaccgacagg gtcaccggca ccaacaaggg
     5281 catctcgccg gtgcctatca acctccgcgt ctactcgccg cacgtgctga acctgaccct
     5341 ggtggacctg cccggaatga ccaaggtccc ggtgggggac caacctcccg acatcgagtt
     5401 ccagatccga gacatgctta tgcagtttgt caccaaggag aactgcctca tcctggccgt
     5461 gtcccccgcc aactctgacc tggccaattc tgacgccctc aaggtcgcca aggaggtgga
     5521 cccccagggc cagcgcacca tcggggtcat caccaagctg gacctgatgg acgagggcac
     5581 agatgcccgt gatgtgctgg agaacaagct gctccccctg cgcagaggct acattggagt
     5641 ggtgaaccgg agccagaagg acattgatgg caagaaggac attaccgccg ccttggctgc
     5701 tgaacgaaag ttcttcctct cccatccatc ttatcgccac ttggctgacc gtatgggcac
     5761 gccctacctg cagaaggtcc tcaatcagca actgacgaac cacatccggg acacactgcc
     5821 ggggctgcgg aacaagctgc agagccagct actgtccatt gagaaggagg tggaggaata
     5881 caagaacttc cgccctgatg acccagctcg caagaccaag gccctgctgc agatggtcca
     5941 gcagtttgcc gtagactttg agaagcgcat tgagggctca ggagatcaga tcgacaccta
     6001 cgaactgtca gggggagccc gcattaaccg aatcttccac gagcgcttcc ctttcgagct
     6061 ggtcaagatg gagtttgatg agaaggaact ccgaagggag atcagctatg ctatcaagaa
     6121 tatccatggc attagaacgg ggctgtttac cccagacatg gcctttgaga ccattgtgaa
     6181 aaagcaggtg aagaagatcc gagaaccgtg tctcaagtgt gtggacatgg ttatctcgga
     6241 gctaatcagc accgttagac agtgcaccaa gaagctccag cagtacccgc ggctacggga
     6301 ggagatggag cgcatcgtga ccacccacat ccgggagcgc gagggccgca ctaaggagca
     6361 ggtcatgctt ctcatcgata tcgagctggc ttacatgaac accaaccatg aggacttcat
     6421 aggctttgcc aatgctcagc agaggagcaa ccagatgaac aagaagaaga cttcagggaa
     6481 ccaggatgag attctggtca tccgcaaggg ctggctgact atcaataata ttggcatcat
     6541 gaaagggggc tccaaggagt actggtttgt gctgactgct gagaatctgt cctggtacaa
     6601 ggatgatgag gagaaagaga agaaatacat gctgtctgtg gacaacctca agctgcggga
     6661 cgtggagaag ggctttatgt cgagcaagca tatctttgcc ctctttaaca cggagcagag
     6721 gaatgtctac aaggattatc ggcagctgga gctagcctgt gagacacagg aggaggtgga
     6781 cagctggaag gcctccttcc tgagggctgg cgtgtaccct gagcgtgttg gggacaaaga
     6841 gaaagccagc gagaccgagg agaatggctc cgacagcttc atgcattcca tggacccaca
     6901 gctggaacgg caagtggaga ccatccggaa tcttgtggac tcatacatgg ccattgtcaa
     6961 caagaccgtg agggacctca tgcccaagac catcatgcac ctcatgatta acaataccaa
     7021 ggagttcatc ttctcggagc tgctggccaa cctgtactcg tgtggggacc agaacacgct
     7081 gatggaggag tcggcggagc aggcacagcg gcgcgacgag atgctgcgca tgtaccacgc
     7141 actgaaggag gcgctcagca tcatcggcaa catcaacacg accaccgtca gcacgcccat
     7201 gcccccgccc gtggacgact cctggctgca ggtgcagagc gtaccggccg gacgcaggtc
     7261 gcccacgtcc agccccacgc cgcagcgccg agcccccgcc gtgcccccag cccggcccg
//
