LOCUS       sequence-171243-        9039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human MAP3K2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Mazur PK, Reynoird N, Khatri P, Jansen PW, Wilkinson AW, Liu S, 
            Barbash O, Van Aller GS, Huddleston M, Dhanak D, Tummino PJ, Kruger 
            RG, Garcia BA, Butte AJ, Vermeulen M, Sage J, Gozani O
  TITLE     SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer.
  JOURNAL   Nature. 2014 Jun 12;510(7504):283-7. doi: 10.1038/nature13320. Epub 
            2014 May 21.
  PUBMED    24847881
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13320"; journalName: "Nature"; date: "2014-06-12- 
            12"; volume: "510"; issue: "7504"; pages: "283-7"
COMMENT     Sequence Label: pMSCV-MAP3K2
FEATURES             Location/Qualifiers
     source          1..9039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..85
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             285..801
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    865..1206
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1273..1689
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1619..1641
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1657..1673
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1702..1711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1762..1782
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1822..1851
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1870..1894
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3822..3846)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3898..3921)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4250..4269
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4335..5360
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4335..5360
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5718..6232
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6394..6410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6418..6424,6425..6442,6443..6448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6463..6484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6601..6618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6852..6871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7531..8322,8323..8391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8154..8173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8392..8496)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8564..8582
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8620..8642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8742..8761
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8955..8977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
       61 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      121 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg
      181 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
      241 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
      301 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
      361 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
      421 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
      481 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
      541 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
      601 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
      661 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
      721 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
      781 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
      841 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
      901 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttagctaac
      961 tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac
     1021 cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag
     1081 tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa
     1141 cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt
     1201 ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     1261 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     1321 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     1381 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     1441 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     1501 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     1561 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
     1621 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
     1681 ctaggcgccg gaattagatc tgccgccatg gattacaagg atgacgacga taaggattat
     1741 gatattccaa ctactgctag cgagaatttg tattttcagg gtgagctcaa aaccgcggct
     1801 cttgcgcaac acgatgaagc cgagcagaaa ctcatctctg aagaggatct gggatccctc
     1861 gaggttatca caagtttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgact
     1921 ggatccggta ccgaattcat ggatgatcag caagctttga actcaatcat gcaagatttg
     1981 gctgtccttc ataaggccag tcgaccagca ttatccttgc aggaaaccag aaaagcaaaa
     2041 tcttcatcac caaaaaaaca gaatgatgtc cgagtcaaat ttgaacatag aggagaaaaa
     2101 agaatccttc agttccccag accagttaaa ctggaagatc tgagatctaa agctaaaatt
     2161 gcctttggac agtctatgga tctacattat accaataacg agttggtaat tccattaact
     2221 actcaagatg acttggacaa agctgtggaa ctgctggatc gtagtattca tatgaagagc
     2281 ctcaagatat tacttgtaat aaatggaagt acacaggcta ctaatttaga accattgcca
     2341 tcactagaag atttggataa tacagtattt ggagcagaga ggaaaaaacg gctatctata
     2401 ataggtccta ctagtagaga tagaagttct cctcccccag gttacattcc agatgaatta
     2461 caccaggttg cccggaatgg gtcattcact agtatcaaca gtgaaggaga gttcattcca
     2521 gagagcatgg accaaatgct ggatccatta tctttaagca gccctgaaaa ttctggctca
     2581 ggaagttgtc catcacttga tagtcctttg gatggagaga gctatccaaa atcacgaatg
     2641 cctagggctc agagctaccc agataatcat caggaatttt cagactatga taaccctatc
     2701 tttgagaaat ttggaaaagg aggaacatat ccaagaaggt atcatgtttc atatcatcat
     2761 caagagtata atgatggtcg taaaactttt ccaagagcta gaaggaccca ggggaccagc
     2821 ttacggtctc ctgtgagttt cagtcctact gatcattcct taagcactag tagtggaagc
     2881 agtatcttta ccccagagta tgatgatagt cgaataagaa gaaggggaag tgacatagac
     2941 aatcctactt tgaccgtaat ggacatcagc ccacccagcc gttcacctcg agctccgacc
     3001 aactggagat tgggcaaact gcttggccaa ggagcctttg gaagggtcta cctctgttat
     3061 gatgttgata caggaagaga attggctgtt aagcaagttc aatttgaccc cgatagtcct
     3121 gagaccagca aggaagtaaa tgcacttgag tgtgaaattc agttgctgaa aaacttgcta
     3181 catgagcgaa ttgttcagta ttatggctgt ttgagggatc cccaggaaaa aacactttcc
     3241 atatttatgg aatatatgcc agggggttca attaaggacc aattaaaagc atatggcgct
     3301 cttactgaga atgtgactag gaaatacacc cgtcagattc tggagggtgt ccattatttg
     3361 cacagtaata tgattgtcca tagagatatc aaaggcgcaa atatcctgcg agattcaaca
     3421 ggcaacgtca aactaggaga ttttggggcc agcaaacggc ttcagaccat ctgtctctca
     3481 gggacaggaa tgaagtctgt cacgggcaca ccatactgga tgagccctga agtcatcagt
     3541 ggagaaggct atggaagaaa agcagacatc tggagtgttg catgtactgt ggtagaaatg
     3601 ctaactgaaa agccgccttg ggctgaattt gaagcaatgg ctgccatctt taaaatcgcc
     3661 actcagccaa caaacccaaa gctgccacct catgtctcag actatactcg agatttcctc
     3721 aaacggattt ttgtagaggc caaactgaga ccttcagctg atgaactctt aaggcacatg
     3781 tttgtgcatt atcactaggc ggccgcactc gagatatcta gacccagctt tcttgtacaa
     3841 agtggtgata acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     3901 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     3961 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4021 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4081 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4141 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     4201 tgggctcaga ggctgggaag gggcacccga aggtcctccg gaggcccggc attctgcacg
     4261 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     4321 tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     4381 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     4441 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     4501 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     4561 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     4621 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     4681 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     4741 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     4801 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     4861 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     4921 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     4981 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5041 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     5101 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     5161 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     5221 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     5281 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     5341 gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc
     5401 tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg
     5461 tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt
     5521 cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac
     5581 gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg
     5641 ggtgcagatc cgtcgacctg cagccaagct tatcgataaa ataaaagatt ttatttagtc
     5701 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     5761 cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt
     5821 aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     5881 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
     5941 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     6001 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     6061 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg
     6121 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     6181 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca
     6241 gtttcttgaa gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga
     6301 ctcaattagc cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg
     6361 acagcgcaga aagagctggg gagaattgtg aaattgttat ccgctcacaa ttccacacaa
     6421 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6481 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8521 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8581 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8641 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     8701 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     8761 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8821 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8881 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8941 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc
     9001 aaggagatgg cgcccaacag tcccccggcc acggggcct
//
