LOCUS       sequence-171250-        9135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-guide Perturb-seq vector backbone; modified human U6 promoter; 
            constant region 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE,
            Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit 
            A, Parnas O, Regev A, Weissman JS
  TITLE     A Multiplexed Single-Cell CRISPR Screening Platform Enables 
            Systematic Dissection of the Unfolded Protein Response.
  JOURNAL   Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 
            10.1016/j.cell.2016.11.048.
  PUBMED    27984733
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1867-1882.e21"
COMMENT     Sequence Label: pMJ117
FEATURES             Location/Qualifiers
     source          1..9135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             93..273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     782..798
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     783..799
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    802..1390
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             908..919
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1317..1337
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1446..1479
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1546..1566
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1717..1736
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        1979..2190
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2191..3129
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3086..3106
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3150..3746
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3150..3169)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3646..3666
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3756..3809
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3822..4520
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(4564..4581)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4570..4794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4853..4970
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5268..5312)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5497..5730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6227..6352
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6399..6579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6597..6795)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6622..6642)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6797..7176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7348..7367
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7442..7546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7547..7615,7616..8407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7765..7784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8578..9135,1..31)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9067..9086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
       61 cctggccttt tgctggcctt ttgctcacat gctgctagag attttccaca ctgactaaaa
      121 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
      181 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
      241 ccaggctcag atctggtcta accagagaga cccagtacag tccggatgca gctctcgggc
      301 catgtgatga aatgctaggc ggctgtcaaa cctccactct aatacttctc tctccgggtc
      361 atccatccca tgcaggctca cagggtgtaa caagcgggtg ttctctcctt cattggcttc
      421 ttctaccttc tcttgctcaa ctggtactag cttgtagcac catccaaagg tcagtggata
      481 tctgatccct ggccctggtg tgtagttctg ccaatcaggg aagtagcctt gtgtgtggta
      541 gatccacaga tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtccccc
      601 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
      661 tacctgaggt gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca
      721 atcagcattg gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg
      781 atcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga ctcgtctgag
      841 ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt
      901 ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg
      961 caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc acggaattgt
     1021 cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg
     1081 ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa
     1141 tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga
     1201 caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa
     1261 tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata
     1321 gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca
     1381 gaggttgatt atcgataagc ttgatatcga attgtaccta gtggaaccgg aacccttaaa
     1441 catgtataac ttcgtataat gtatgctata cgaagttatt aggtccctcg acgttaacgc
     1501 tcgaatctac actcagctat ggcgcgcccc aaggtcgggc aggaagaggg cctatttccc
     1561 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     1621 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1681 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1741 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aagccagaaa catggaccag
     1801 gatgggcacc acccgtttca gagctaagca caagagtgca tagcaagttg aaataaggct
     1861 agtccgttta caacttgaaa aagtggcacc cgagtcgggt gctttttttc tcgagtacta
     1921 ggatccatta ggcggccgcg tggataaccg tattaccgcc atgcatgtgc ccgtcagtgg
     1981 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2041 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2101 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2161 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     2221 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     2281 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2341 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2401 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2461 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2521 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     2581 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     2641 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     2701 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     2761 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     2821 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     2881 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgcg cttttggagt
     2941 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3001 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     3061 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     3121 ccatttcagg tgtcgtgagc tagcccacca tgaccgagta caagcccacg gtgcgcctcg
     3181 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3241 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     3301 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3361 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3421 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3481 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     3541 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3601 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3661 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     3721 ggtgcatgac ccgcaagccc ggtgccggat cgggagaggg cagaggaagt ctgctaacat
     3781 gcggtgacgt cgaggagaat cctggcccac cggtcgccac cagcgagctg attaaggaga
     3841 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
     3901 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     3961 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
     4021 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
     4081 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     4141 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
     4201 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
     4261 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
     4321 gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca
     4381 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
     4441 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
     4501 tggggcacaa gcttaattaa gaattcctag ggcctagagg gcccgtttaa acccgctgat
     4561 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4621 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4681 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4741 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggtctaga
     4801 gcgggcccgg tactaaccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt
     4861 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
     4921 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga
     4981 atactgccat ttgtctgcag aattggcgca cgcagtgccg atccgttcac taatcgaatg
     5041 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
     5101 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     5161 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     5221 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     5281 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     5341 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     5401 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     5461 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     5521 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     5581 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     5641 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     5701 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     5761 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     5821 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     5881 ggccggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     5941 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     6001 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     6061 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     6121 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     6181 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     6241 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     6301 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     6361 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     6421 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     6481 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     6541 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     6601 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     6661 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     6721 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     6781 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     6841 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     6901 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     6961 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     7021 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     7081 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     7141 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     7201 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     7261 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     7321 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7381 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     7441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7561 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7621 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7681 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7741 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7801 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7861 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7921 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7981 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8041 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8101 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8161 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8221 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8281 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8341 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8401 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8461 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8941 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9001 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9061 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9121 gcgtcgattt ttgtg
//
