LOCUS       sequence-171259-       10126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN263
FEATURES             Location/Qualifiers
     source          1..10126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     71..87
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     71..87
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    107..127
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1588..1612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1761..1772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6738..6762)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7809..7829)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7837..7854)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7837..7853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7846..7868)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8181..8203
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8241..8259)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8327..8431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8432..8500,8501..9292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8650..8669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9463..10051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9952..9971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cagcggataa
       61 caatttcaca caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga
      121 aaagttggag ggacttccag ctgtctgcga gccagtccaa aagggagagc caggatccac
      181 cggagagcca ggaccagtcg gaccaccagg accatccgga caaccaggaa acgacggaac
      241 cccaggtaat catttcatag tttttcaatc catatctaat caccacatct ttcaggacaa
      301 cctggaccaa agggaccacc aggaccagac ggcaagccag gagccgacgg aaacccagga
      361 cagccaggac cagtcggacc accaggaacc ccaggagagc gtggaatctg tccaaagtac
      421 tgcgccatcg atggaggaat cttcttcgag gacggaactc gccgctaagc ggatcgcctt
      481 taatttattc tttgccttat gcgttgctca aaataaaaca ttatttcatc caaaataatt
      541 ttttgaggtt ttagatgaat ttgatagttt agcttgtcac tttctcctgt gcgaagaaac
      601 ttgctaagca ggtaagcagc atccttatca agtttcgacc ttatctctca gtagtcgcat
      661 acgtcgtatc cctcatctgc tttgttttag ccacttcaag ggaattcaga tcactttttc
      721 tcatgtgttt tcaaatttac ttttgtggag tgaccttaat tgaccctctc gtcataagtg
      781 gacggtgggt agttacttaa caaatttcga gtacaggata gtgtcaatgg catgagacat
      841 gtagtagcca agcaattatg gcgaataagt ttcaaaaacc aacattctgt caaatacaaa
      901 gacctgcaac attagagaat tcattaaata catcttcgag gaaatcaaat tttcccgtag
      961 acgatcccgt agacctactt gtacaatatg ttctagatat acactccgaa cttaaaatat
     1021 ctcgaacatt tgtgttgaat atttatttta attctaggtt tttcaaattc cgcacgattt
     1081 ttgccatact actgcccaat agtttacatt tattgggcct ggatctgtaa ggcacttttg
     1141 ggagctccct ttcttttcct tcaaattacc catacagatc tataaatcac aatttaatac
     1201 aagattttat tcaagttaaa aacataaaag gggctttgcg gtggtcccca tttcaccaga
     1261 gaactataaa aaatataaat ttaacatcaa atgttgcaat tgattccctc tcaaagctgc
     1321 ttgaagaacc ctgattctgt caagcctatg aagatttaaa aaaaattggg aagaccctta
     1381 gttccaaaca agtgtcgttg acccagtagg cgatattctg aaaagtcata aaatggggtt
     1441 gctaaaaaat tggtcgctaa cttacattta gctaggaatg ttaattggaa taactcataa
     1501 tttacagtaa aattatcaaa aaatagcaaa aatcaataga ggaccgctac acaggcatcc
     1561 aacagtacga gttctgcgtt tttgaattca agtttgtaca aaaaagcagg ctctttgagc
     1621 caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa aatgtttatt
     1681 ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca gaaaccggga
     1741 tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac aaaccggtta
     1801 ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca aattaagtta
     1861 catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta ttctgttcct
     1921 attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc ttggatagtg
     1981 tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt ccgctcgttc
     2041 ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct tctcaatctt
     2101 cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac cctcattcat
     2161 ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc tattccacgt
     2221 cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat tcgtttttgg
     2281 gattcacccc gcggttcgca ataggtttct ttttcaaatt attagcgtta taaatagaaa
     2341 aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta catgtactca
     2401 aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca ttatcgtatt
     2461 tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt ggcaccctct
     2521 aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt ccgttttcag
     2581 ccgcttccaa ccaaaccgat atgaaagccg agcaacaacg atcgattcca cccggctcgg
     2641 cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac taactcttga
     2701 tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt tccagatgcc
     2761 acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg cgaagaaagc
     2821 tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta tcttgctccc
     2881 tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct gcgaacatct
     2941 ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa gtgcttttcg
     3001 agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag tcggcaagat
     3061 acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg
     3121 aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt cacttcttca
     3181 aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg aattcacgaa
     3241 acaactgtga acatatctat gagttccctc aactctctca acaactcagt gagtcattat
     3301 tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg atcaacaatc
     3361 caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc atgcacaaca
     3421 aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg ataattattc
     3481 tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg tctagtttgt
     3541 tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt ccccccttgt
     3601 ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca cggaaatctg
     3661 ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga ttttttcatt
     3721 agagaatgtc tagaactagg cccggggaaa gccgggttac cataagaaaa aaaattacat
     3781 tttttctcga atagtagaaa catattgcaa gactattttt cattatttca gaacacacac
     3841 tggagagaga ccgtatccat gtgctcaatg tgagaaggca ttcaccgaca gctcaactct
     3901 tacaaaacat ctgagaaccc atactggaca taaaccttat gtttgctcaa tttgtatgat
     3961 gaaattcaca cagagtggga acttgcatag gcacatgaaa actcataaat agtttttcct
     4021 attatggaag taatgtttct aaatgaatat tgccattttc tgcaaaatat tttacaatcc
     4081 acaagtctcg aacaaattta atcaatgtct tgttttgata caatttaaca aattcactgt
     4141 ggagaggatt tactataaaa acgttttaca ccagcaaaaa atcaatagaa catttacatg
     4201 ataaggagaa tatataaaaa gggggcatta taatttactg atacaatatg gacaacattt
     4261 gaaggatatg cagatacata cgcgttagat acgacggttg atgtaggagg agaggtagtc
     4321 gaggacctct tgggagatga ttccgttcca ggctgggtaa cggatggatc cctcggcgga
     4381 tcccttgagt tgctcgatgt gttgccactc ctcgattggg ttggtctcgt ccttgagggc
     4441 gatcatctcc ttggagattg ggtcgtaggc gtagtaacgg gagacgaggg cgaagtagtg
     4501 gtctgggatg gcggtgattt ggtgggtgta ggtggtacgg gcgacggcgg aggcacgctt
     4561 gtcggaccag tttccgacga cgttggtgag ctcggtgagt ccctgaaaat ttaaatcaga
     4621 ttagttagtt agtaaaatca tgtttaaact taccttcatg gagaggaagg aggtcatgag
     4681 gtgacgtccg atgtgggact ttggtccgtt cttgatggcg aagatggagt atggggcgtt
     4741 cttcttgagg gccttgttgt aggaacggac gaggttgtcc ttgaggagtt ggtactcttg
     4801 cttgttggag gaggagtttc cggtacggtt gacacgcttg aggactggct cggagttacg
     4861 gaggaactcg tcgaggtaga cgagtgggtc gatacgtcca cgggcggaga agaagtagat
     4921 gtgacgggag acggaggtct tggtctcggt gacgaggcat tggatgatga ctccgaggta
     4981 cttgttttgg acgagcttga aggactttgg gtcgacgttc ttgatgtcgg agaaacgtcc
     5041 gcagttgatg aaggtggcga ggaagaggaa ttggtagagg gtcttggtct tggtgaaacg
     5101 ggaggtgtac tcgaaggagt tgaggatctt ctcggtgatc tcccagatgg actctccctc
     5161 ggagaggagg gccttgagca tcttcttgga gtgggagttt cccttgtcgg cctcctcgga
     5221 ggactcgaat tggagttgga gggaggagac gatgtcggtg atgtcggatt ggtgcttttg
     5281 tccgtagtat gggatgatgg tgaactccca ggctgggatg agcttctgaa aatttaaata
     5341 atcagggtta gttagtatat atatgtttaa acttaccttg agggaggcct cgaggatggt
     5401 ggccttttgg gtcttgtact tgaattggag ggacttgttg acgatgtcga aggagaggga
     5461 gttggagatg atggtgttgt aggacatgaa ggtggcacgc ttgatggcgg ttccgttgtg
     5521 ggtgatcatc cagcagaggt aggtgagctc ggcggcgcag agggcgatct tctctccgga
     5581 tggacgctcg aaacgctcga cgaattgacg gacgaggact tttggtggtg tcttacagag
     5641 aatgtcgaat tgtggcattt ttggtacctt ctgtgtctga cgatcgaggg ttaaaatgaa
     5701 aagtggtgga gagaagtcaa ccgctcggtg ggatgcgcga gcctggagat aactttgatt
     5761 ttcggcgagc ccttcttttt ccctgggtgg gtggattggg aggcggggat atccaattgt
     5821 aattctaggt agctgaaaag tgtctgcaca ctgttctctg accatcgacg caggaaaagt
     5881 ctctttctca agtgcaaata gcaaaaatct tctttcttcc cccccatctt ccgagcaagg
     5941 cgcccccacg gatagttgct cgctggcggt agggagcgaa gagagcagag aacctcaacg
     6001 ggatagtggg gatacgagaa aagaaacggc attttaggtt aactaaagat agtgaagaag
     6061 ccggctcaat gcagagaggc agacatccga cggcaaacaa cgagagaatt ttatccagaa
     6121 caatgagtac agtgtgtgcg cgcaaagtgc tcttcctgcc attttgctac tcttatccac
     6181 acaaccactt tagcgattac caacctatct tgagacttat ctttattttc tcttcttttg
     6241 ccgacgaaac gggagagagg ggcaaaaaat gaagaatcgg gggcactgat taacacactt
     6301 actttttttt aggctgaagc acacattgct gttcggggtg taaagttcag cagaattcaa
     6361 agcaaaagac cacccgcatc ggtagtcaat attgatctgg aagattatta aaaattgtca
     6421 gagtgagcca tatgcatatg aatttaattc atacataaca tttataagac aggaataaat
     6481 accaaagtga agttattatt tggtcgatta tcactttagc atagtgaaag tatccctcaa
     6541 tgtctctact tgtaaactca gttgatttac aaacgttgtt ttattgatta taaaattagt
     6601 tttatggaca tttaaagcaa aggatactac tcagatataa aatgcctgca ggtagcggcc
     6661 gcatgctaca gccggctcct cgagacgtac ggtgcgcgcg atgcattcga agatctgccc
     6721 actagtgagt cgtattaacc cagctttctt gtacaaagtg gatagaatca agcatgctcc
     6781 gcgtttgctg tactatttct cgcagagcag ttttttttct cacactttat tattctgtac
     6841 tgcccctcca cgctataatt ttttgcatag agggccataa aggaaaacgg tacggtaaaa
     6901 ataatgggtc ctttttaccg ttgtgtagat tgtttctaac gggatgcaaa ttttcactaa
     6961 aactctggaa aactgatgga attctacggg cagccggcaa ttttagatat ttgaagaatc
     7021 cttcttgatc agtgccaagc tttcccatgg accaaagatg tggatccaag atcaaatgca
     7081 caggatctga actcctttgt tccaaaaaca aggatttctt ttctatagtt tgacctaaat
     7141 attatagtag aacatataca tttgatgatt tcatgaataa tttattgtta aaaaataata
     7201 catgtgttaa aaaataattc taaaaaatta attggagcgt ccttggcgga tatggcggat
     7261 tggaaggtca taagtaactc gaactaacct gtaaatataa ttaatttaga agtaatcatt
     7321 ttgaaatgtt tctaaatttt tgtaaacgta atcaatgatt tacttacaaa atttttcgaa
     7381 atattagtag aaatataaaa aaccagtaaa attcaaaaga ggccagccac acatgcatct
     7441 tacaatacga atgatgcgtt ttttgtcgcc tcggagaagg aatcaagcag gctccgcgtt
     7501 ttctgtacta tttctcccag agccgtttat tttgcatagg gggtgggtca taaacggaga
     7561 cgttttggta taaaatttgc cccttcttcc accgttgaaa aaattggaaa atgaaccggg
     7621 acaggaaagg ggatttcagg ataatggttc aattatcgat gctactgtca tatctgagta
     7681 gtggatttga gcaaggtttc caaattttga gtacaagcgt ctagtgcatg tgtcaaaaaa
     7741 gtcgggcaaa aattagtttt aatcaataag gtttaaagat cagaaatttt tgtctgacct
     7801 aatgggccca actttattat acatagttga taattcactg gccgtcgttt tacaacgtcg
     7861 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     7921 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7981 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8041 ccgcatatgg tgcactctca gtgcaatctg ctctgatgcc gcatagttaa gccagccccg
     8101 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8161 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     8221 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     8281 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggtcaga
     9181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10081 cctggtcttt tgctggcctt ttgctcacat gttctttcct gcgtta
//
