LOCUS       sequence-171260-        5157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Morales-Martinez A, Dobrzynska A, Askjaer P
  TITLE     Inner nuclear membrane protein LEM-2 is required for correct nuclear
            separation and morphology in C. elegans.
  JOURNAL   J Cell Sci. 2015 Mar 15;128(6):1090-6. doi: 10.1242/jcs.164202. Epub
            2015 Feb 4.
  PUBMED    25653391
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.164202"; journalName: "J Cell Sci"; date: "2015-03-15- 
            15"; volume: "128"; issue: "6"; pages: "1090-6"
COMMENT     Sequence Label: pBN41
FEATURES             Location/Qualifiers
     source          1..5157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     381..398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    515..536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(551..556,557..574,575..581)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    589..605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     594..616
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     613..629
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     613..629
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1683..1711)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2459..2479
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3355..3377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3477..3496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3574..3592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3660..3764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3765..3833,3834..4625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3983..4002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4796..5157,1..227)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
       61 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      481 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      541 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      601 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
      661 cattttatat ctgagtagta tcctttgctt taaatgtcca taaaactaat tttataatca
      721 ataaaacaac gtttgtaaat caactgagtt tacaagtaga gacattgagg gatactttca
      781 ctatgctaaa gtgataatcg accaaataat aacttcactt tggtatttat tcctgtctta
      841 taaatgttat gtatgaatta aattcatatg catatggctc actctgacaa tttttaataa
      901 tcttccagat caatattgac taccgatgcg ggtggtcttt tgctttgaat tctgctgaac
      961 tttacacccc gaacagcaat gtgtgcttca gcctaaaaaa aagtaagtgt gttaatcagt
     1021 gcccccgatt cttcattttt tgcccctctc tcccgtttcg tcggcaaaag aagagaaaat
     1081 aaagataagt ctcaagatag gttggtaatc gctaaagtgg ttgtgtggat aagagtagca
     1141 aaatggcagg aagagcactt tgcgcgcaca cactgtactc attgttctgg ataaaattct
     1201 ctcgttgttt gccgtcggat gtctgcctct ctgcattgag ccggcttctt cactatcttt
     1261 agttaaccta aaatgccgtt tcttttctcg tatccccact atcccgttga ggttctctgc
     1321 tctcttcgct ccctaccgcc agcgagcaac tatccgtggg ggcgccttgc tcggaagatg
     1381 ggggggaaga aagaagattt ttgctatttg cacttgagaa agagactttt cctgcgtcga
     1441 tggtcagaga acagtgtgca gacacttttc agctacctag aattacaatt ggatatcccc
     1501 gcctcccaat ccacccaccc agggaaaaag aagggctcgc cgaaaatcaa agttatctcc
     1561 aggctcgcgc atcccaccga gcggttgact tctctccacc acttttcatt ttaaccctcg
     1621 atcgtcagac acagaaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg
     1681 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1741 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1801 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt
     1861 atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa tgcttctcga
     1921 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     1981 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac
     2041 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag
     2101 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2161 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     2221 aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact aactaatctg
     2281 atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc gttcaactag
     2341 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     2401 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     2461 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     2521 gcccggggga tcggtggagc tccgcatcgg ccgctgtcat cagatcgcca tctcgcgccc
     2581 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     2641 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     2701 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     2761 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     2821 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     2881 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     2941 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3001 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3061 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3121 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3181 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3241 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttctac
     3301 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3361 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3421 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3481 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     3541 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     3601 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4621 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacct
//
