LOCUS       sequence-171264-       14873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LentiCRISPR v2 was modified into an all-in-one dox inducible system.
            The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter 
            drives constitutive expression of an sgRNA targeting human RB1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: TLCV2-RB1
FEATURES             Location/Qualifiers
     source          1..14873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..2856
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2608..2628
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2779..2798
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2878..2953
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2990..3304
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2998..3016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3034..3052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3176..3194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3212..3230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3277..3301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3330..3339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3336..7439
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3336..7439
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7440..7487
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7488..7511
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7521..7574
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7575..7577,7578..8291)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7575..7577,7578..8288)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7878..7897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8225..8246
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8302..8513
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8535..9131
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8535..8554)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9031..9051
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9141..9197
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(9198..9815,9816..9821,9822..9857,9858..9860,9861..9896,
                     9897..9899,9900..9935,9936..9944)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9965..10553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10018..10038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10436..10447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10625..10858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10884..10901)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10890..11114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11160..11588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11247..11266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11457..11478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11597..11617)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11602..11931
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11782..11917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11844..11863
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11979..12026
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12045..12419
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12549..12670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12586..12605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12640..12659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12719..12735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12719..12735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12732..12754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12743..12759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12767..12773,12774..12791,12792..12797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12812..12833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12950..12967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13121..13709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13201..13220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13880..14671,14672..14740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14503..14522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14741..14845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggct ctgggtcctc ctcaggagtt
     2881 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2941 accgagtcgg tgcttttttg aattcacttt ggccgcgaat cgatatgtcg agtttactcc
     3001 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
     3061 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     3121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
     3181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     3241 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     3301 cgcctggaga ataccggttc tagagcgctg ccaccatgga caagaagtac agcatcggcc
     3361 tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
     3421 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
     3481 gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca
     3541 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
     3601 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
     3661 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
     3721 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
     3781 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
     3841 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
     3901 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
     3961 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     4021 tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     4081 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     4141 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     4201 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     4261 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     4321 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     4381 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     4441 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     4501 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     4561 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     4621 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     4681 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     4741 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     4801 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     4861 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     4921 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     4981 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     5041 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     5101 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     5161 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     5221 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     5281 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     5341 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     5401 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     5461 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     5521 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     5581 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     5641 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     5701 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     5761 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     5821 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     5881 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     5941 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     6001 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     6061 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     6121 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     6181 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     6241 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     6301 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     6361 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     6421 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     6481 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     6541 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     6601 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     6661 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     6721 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     6781 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     6841 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     6901 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     6961 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     7021 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     7081 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     7141 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     7201 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     7261 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     7321 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     7381 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca
     7441 agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaagat tacaaagacg
     7501 atgacgataa gggatccgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     7561 agaatcctgg cccagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     7621 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     7681 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     7741 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     7801 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7861 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7921 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7981 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     8041 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     8101 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     8161 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     8221 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     8281 tgtacaagta agctagcatc cgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     8341 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     8401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     8461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagactgcga
     8521 tcgcaatgta cagtatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     8581 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     8641 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     8701 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     8761 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     8821 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     8881 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg
     8941 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     9001 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     9061 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     9121 agcccggtgc cggttccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg
     9181 aagagaatcc tggaccgatg tctagactgg acaagagcaa agtcataaac ggagctctgg
     9241 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     9301 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     9361 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
     9421 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     9481 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     9541 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     9601 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     9661 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
     9721 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
     9781 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
     9841 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     9901 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactcgag cgcgttaagt
     9961 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    10081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    10141 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    10201 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    10261 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    10321 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    10381 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    10441 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    10501 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
    10561 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    10621 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
    10681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
    10861 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    10921 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    10981 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    11041 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    11101 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    11161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    11221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    11281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    11341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    11401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    11461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    11521 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    11581 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    11641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    11701 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    11761 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    11821 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    11881 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    11941 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
    12001 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
    12061 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
    12121 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
    12181 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
    12241 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
    12301 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
    12361 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
    12421 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    12481 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    12541 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    12601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12661 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    12721 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    12781 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    12841 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    12901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    12961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    13021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    13081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    13321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    13381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    13441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    13501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    13561 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    13621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    13681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    13741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    13801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    13861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    13921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    13981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    14041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    14101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    14161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    14221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    14281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    14341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    14401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    14461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    14521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    14581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    14641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    14701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    14761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    14821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
