LOCUS       sequence-171267-        8928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent human protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 mCherry-Rtn4HD
FEATURES             Location/Qualifiers
     source          1..8928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(1454..2027,2028..2030,2031..2039)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1620..1637)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1847..1866
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2040..2435
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2445..2498
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2502..2522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2548..3136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2601..2621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3019..3030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3284..3464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3511..3529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3597..3701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3702..3770,3771..4562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3920..3939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5475..5492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5732..5753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5768..5773,5774..5791,5792..5798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5806..5822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5811..5833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5830..5846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5830..5846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5896..5915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6107..6740
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6787..6912
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7409..7642
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7827..7871
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8167..8284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8336..8639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8640..8843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8794..8814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8840..8864
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 attcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      721 tctcgagcac aagcagagat agagagcata gttaaaccca aagttcttgt gaaagaagct
      781 gagaaaaaac ttccttccga tacagaaaaa gaggacagat caccatctgc tatattttca
      841 gcagagctga gtaaaacttc agttgttgac ctcctgtact ggagagacat taagaagact
      901 ggagtggtgt ttggtgccag cctattcctg ctgctttcat tgacagtatt cagcattgtg
      961 agcgtaacag cctacattgc cttggccctg ctctctgtga ccatcagctt taggatatac
     1021 aagggtgtga tccaagctat ccagaaatca gatgaaggcc acccattcag ggcatatctg
     1081 gaatctgaag ttgctatatc tgaggagttg gttcagaagt acagtaattc tgctcttggt
     1141 catgtgaact gcacgataaa ggaactcagg cgcctcttct tagttgatga tttagttgat
     1201 tctctgaagt ttgcagtgtt gatgtgggta tttacctatg ttggtgcctt gtttaatggt
     1261 ctgacactac tgattttggc tctcatttca ctcttcagtg ttcctgttat ttatgaacgg
     1321 catcaggcac agatagatca ttatctagga cttgcaaata agaatgttaa agatgctatg
     1381 gctaaaatcc aagcaaaaat ccctggattg aagcgcaaag ctgaatgagc ggccgctaag
     1441 taagtaagat ccgcccctct ccctcccccc ccctaacgtt actggccgaa gccgcttgga
     1501 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     1561 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     1621 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     1681 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     1741 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     1801 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     1861 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     1921 ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     1981 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
     2041 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     2101 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     2161 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     2221 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     2281 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     2341 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     2401 gtgaattgct gccctctggt tatgtgtggg agggcggtag tggcgagggc agaggaagtc
     2461 ttctaacatg cggtgacgtg gaggagaatc ccggccctgc tccaaagaag aagcgtaagg
     2521 taaatcgatc gctagtcgag agatcctaat caacctctgg attacaaaat ttgtgaaaga
     2581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2941 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3121 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     3181 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3241 agatcacctg caggacaggc gcgccctgct ttttgcttgt actgggtctc tctggttaga
     3301 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3361 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3421 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcacccggg cgattaagga
     3481 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3541 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3601 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5461 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5521 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     5581 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5641 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     5701 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5761 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5821 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     5881 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     5941 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     6001 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     6061 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     6121 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6181 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6241 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     6301 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     6361 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     6421 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     6481 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     6541 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6721 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6781 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6841 cggcgactgc agagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6901 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6961 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7021 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7081 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7141 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7201 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     7261 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7321 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7381 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7441 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7501 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7561 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7621 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7681 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7741 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7801 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7861 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7921 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7981 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8041 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8101 ttcgattagt gaacggatct cgacggtatc gccgaattct caacaatagg cagtattcat
     8161 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     8221 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     8281 ttttcgggtt tattactcgg acagcagaga tccagtttgg actagtggag ttccgcgtta
     8341 cataacttac gcaaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8401 caataatgac caatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8461 tggagtattt acgcaaaact gcccacttgg cagtacatca agtgtatcat ttgccaacaa
     8521 cgccccctat tgacgtcaat gacgcaaaat ggcccgcctg gcattatgcc cagtacatga
     8581 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8641 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     8701 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8761 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     8821 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     8881 cacgctgttt tgacctccat tcaagacacc ggcggcctta attaaacc
//
