LOCUS       sequence-171268-       11817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent human protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 GFP-Rtn4a
FEATURES             Location/Qualifiers
     source          1..11817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    join(4343..4916,4917..4919,4920..4928)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4509..4526)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4736..4755
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4929..5324
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5334..5387
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5391..5411
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5437..6025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5490..5510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5908..5919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6173..6353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6400..6418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6486..6590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6591..6659,6660..7451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6809..6828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7622..8210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8364..8381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8621..8642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8657..8662,8663..8680,8681..8687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8695..8711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8700..8722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8719..8735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8719..8735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8785..8804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8996..9629
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9676..9801
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10298..10531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10716..10760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11056..11173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11225..11528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11529..11732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11683..11703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11729..11753
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
       61 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
      181 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
      241 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
      301 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
      361 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
      421 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
      541 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
      601 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
      661 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtcc
      721 ggactcagat ctcgagctat ggaagacctg gaccagtctc ctctggtctc gtcctcggac
      781 agcccacccc ggccgcagcc cgcgttcaag taccagttcg tgagggagcc cgaggacgag
      841 gaggaagaag aggaggagga agaggaggac gaggacgaag acctggagga gctggaggtg
      901 ctggagagga agcccgccgc cgggctgtcc gcggccccag tgcccaccgc ccctgccgcc
      961 ggcgcgcccc tgatggactt cggaaatgac ttcgtgccgc cggcgccccg gggacccctg
     1021 ccggccgctc cccccgtcgc cccggagcgg cagccgtctt gggacccgag cccggtgtcg
     1081 tcgaccgtgc ccgcgccatc ccagctgtct gctgccgcag tctcgccctc caagctccct
     1141 gaggacgacg agcctccggc ccggcctccc actcctcccc cggccagcgt gagcccccag
     1201 gcagagcccg tgtggacccc gccagccccg gctcccgccg cgcccccctc caccccggcc
     1261 gcgcccaagc gcaggggctc ctcgggctca gtggatgaga ccctttttgc tcttcctgct
     1321 gcatctgagc ctgtgatacg ctcctctgca gaaaatatgg acttgaagga gcagccaggt
     1381 aacactattt cggctggtca agaggatttc ccatctgtcc tgcttgaaac tgctgcttct
     1441 cttccttctc tgtctcctct ctcagccgct tctttcaaag aacatgaata ccttggtaat
     1501 ttgtcaacag tattacccac tgaaggaaca cttcaagaaa atgtcagtga agcttctaaa
     1561 gaggtctcag agaaggcaaa aactctactc atagatagag atttaacaga gttttcagaa
     1621 ttagaatact cagaaatggg atcatcgttc agtgtctctc caaaagcaga atctgccgta
     1681 atagtagcaa atcctaggga agaaataatc gtgaaaaata aagatgaaga agagaagtta
     1741 gttagtaata acatccttca taatcaacaa gagttaccta cagctcttac taaattggtt
     1801 aaagaggatg aagttgtgtc ttcagaaaaa gcaaaagaca gttttaatga aaagagagtt
     1861 gcagtggaag ctcctatgag ggaggaatat gcagacttca aaccatttga gcgagtatgg
     1921 gaagtgaaag atagtaagga agatagtgat atgttggctg ctggaggtaa aatcgagagc
     1981 aacttggaaa gtaaagtgga taaaaaatgt tttgcagata gccttgagca aactaatcac
     2041 gaaaaagata gtgagagtag taatgatgat acttctttcc ccagtacgcc agaaggtata
     2101 aaggatcgtt caggagcata tatcacatgt gctcccttta acccagcagc aactgagagc
     2161 attgcaacaa acatttttcc tttgttagga gatcctactt cagaaaataa gaccgatgaa
     2221 aaaaaaatag aagaaaagaa ggcccaaata gtaacagaga agaatactag caccaaaaca
     2281 tcaaaccctt ttcttgtagc agcacaggat tctgagacag attatgtcac aacagataat
     2341 ttaacaaagg tgactgagga agtcgtggca aacatgcctg aaggcctgac tccagattta
     2401 gtacaggaag catgtgaaag tgaattgaat gaagttactg gtacaaagat tgcttatgaa
     2461 acaaaaatgg acttggttca aacatcagaa gttatgcaag agtcactcta tcctgcagca
     2521 cagctttgcc catcatttga agagtcagaa gctactcctt caccagtttt gcctgacatt
     2581 gttatggaag caccattgaa ttctgcagtt cctagtgctg gtgcttccgt gatacagccc
     2641 agctcatcac cattagaagc ttcttcagtt aattatgaaa gcataaaaca tgagcctgaa
     2701 aaccccccac catatgaaga ggccatgagt gtatcactaa aaaaagtatc aggaataaag
     2761 gaagaaatta aagagcctga aaatattaat gcagctcttc aagaaacaga agctccttat
     2821 atatctattg catgtgattt aattaaagaa acaaagcttt ctgctgaacc agctccggat
     2881 ttctctgatt attcagaaat ggcaaaagtt gaacagccag tgcctgatca ttctgagcta
     2941 gttgaagatt cctcacctga ttctgaacca gttgacttat ttagtgatga ttcaatacct
     3001 gacgttccac aaaaacaaga tgaaactgtg atgcttgtga aagaaagtct cactgagact
     3061 tcatttgagt caatgataga atatgaaaat aaggaaaaac tcagtgcttt gccacctgag
     3121 ggaggaaagc catatttgga atcttttaag ctcagtttag ataacacaaa agataccctg
     3181 ttacctgatg aagtttcaac attgagcaaa aaggagaaaa ttcctttgca gatggaggag
     3241 ctcagtactg cagtttattc aaatgatgac ttatttattt ctaaggaagc acagataaga
     3301 gaaactgaaa cgttttcaga ttcatctcca attgaaatta tagatgagtt ccctacattg
     3361 atcagttcta aaactgattc attttctaaa ttagccaggg aatatactga cctagaagta
     3421 tcccacaaaa gtgaaattgc taatgccccg gatggagctg ggtcattgcc ttgcacagaa
     3481 ttgccccatg acctttcttt gaagaacata caacccaaag ttgaagagaa aatcagtttc
     3541 tcagatgact tttctaaaaa tgggtctgct acatcaaagg tgctcttatt gcctccagat
     3601 gtttctgctt tggccactca agcagagata gagagcatag ttaaacccaa agttcttgtg
     3661 aaagaagctg agaaaaaact tccttccgat acagaaaaag aggacagatc accatctgct
     3721 atattttcag cagagctgag taaaacttca gttgttgacc tcctgtactg gagagacatt
     3781 aagaagactg gagtggtgtt tggtgccagc ctattcctgc tgctttcatt gacagtattc
     3841 agcattgtga gcgtaacagc ctacattgcc ttggccctgc tctctgtgac catcagcttt
     3901 aggatataca agggtgtgat ccaagctatc cagaaatcag atgaaggcca cccattcagg
     3961 gcatatctgg aatctgaagt tgctatatct gaggagttgg ttcagaagta cagtaattct
     4021 gctcttggtc atgtgaactg cacgataaag gaactcaggc gcctcttctt agttgatgat
     4081 ttagttgatt ctctgaagtt tgcagtgttg atgtgggtat ttacctatgt tggtgccttg
     4141 tttaatggtc tgacactact gattttggct ctcatttcac tcttcagtgt tcctgttatt
     4201 tatgaacggc atcaggcaca gatagatcat tatctaggac ttgcaaataa gaatgttaaa
     4261 gatgctatgg ctaaaatcca agcaaaaatc cctggattga agcgcaaagc tgaatgagcg
     4321 gccgctaagt aagtaagatc cgcccctctc cctccccccc cctaacgtta ctggccgaag
     4381 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4441 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4501 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4561 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4621 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4681 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4741 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4801 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4861 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4921 ccacaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     4981 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     5041 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     5101 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     5161 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     5221 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     5281 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcggtagt ggcgagggca
     5341 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgct ccaaagaaga
     5401 agcgtaaggt aaatcgatcg ctagtcgaga gatcctaatc aacctctgga ttacaaaatt
     5461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5581 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5641 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5701 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5761 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5821 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5881 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5941 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6001 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     6061 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6121 acgaagacaa gatcacctgc aggacaggcg cgccctgctt tttgcttgta ctgggtctct
     6181 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6241 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6301 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcacccgggc
     6361 gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     6421 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     6841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8401 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
     8461 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8521 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
     8581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8701 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
     8761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8821 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     8881 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     8941 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     9001 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     9061 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     9121 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     9181 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     9241 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     9301 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     9361 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     9421 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     9481 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9541 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9601 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9661 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9721 ggcgaggggc ggcgactgca gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9781 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9841 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9901 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9961 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10021 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    10081 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    10141 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    10201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    10501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    10561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    10621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    10681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    10741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    10801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    10861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    10921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    10981 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattctc aacaataggc
    11041 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    11101 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11161 aattcaaaat tttcgggttt attactcgga cagcagagat ccagtttgga ctagtggagt
    11221 tccgcgttac ataacttacg caaaatggcc cgcctggctg accgcccaac gacccccgcc
    11281 cattgacgtc aataatgacc aatgttccca tagtaacgcc aatagggact ttccattgac
    11341 gtcaatgggt ggagtattta cgcaaaactg cccacttggc agtacatcaa gtgtatcatt
    11401 tgccaacaac gccccctatt gacgtcaatg acgcaaaatg gcccgcctgg cattatgccc
    11461 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    11521 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    11581 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    11641 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    11701 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
    11761 gacgccatcc acgctgtttt gacctccatt caagacaccg gcggccttaa ttaaacc
//
