LOCUS       sequence-171273-       11808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent human protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 mCherry-Rtn4a
FEATURES             Location/Qualifiers
     source          1..11808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(4334..4907,4908..4910,4911..4919)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4500..4517)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4727..4746
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4920..5315
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5325..5378
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5382..5402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5428..6016
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5481..5501)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5899..5910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6164..6344
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6391..6409)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6477..6581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6582..6650,6651..7442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6800..6819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7613..8201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8355..8372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8612..8633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8648..8653,8654..8671,8672..8678)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8686..8702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8691..8713
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8710..8726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8710..8726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8776..8795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8987..9620
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9667..9792
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10289..10522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10707..10751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11047..11164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11216..11519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11520..11723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11674..11694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11720..11744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 attcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      721 tctcgagcta tggaagacct ggaccagtct cctctggtct cgtcctcgga cagcccaccc
      781 cggccgcagc ccgcgttcaa gtaccagttc gtgagggagc ccgaggacga ggaggaagaa
      841 gaggaggagg aagaggagga cgaggacgaa gacctggagg agctggaggt gctggagagg
      901 aagcccgccg ccgggctgtc cgcggcccca gtgcccaccg cccctgccgc cggcgcgccc
      961 ctgatggact tcggaaatga cttcgtgccg ccggcgcccc ggggacccct gccggccgct
     1021 ccccccgtcg ccccggagcg gcagccgtct tgggacccga gcccggtgtc gtcgaccgtg
     1081 cccgcgccat cccagctgtc tgctgccgca gtctcgccct ccaagctccc tgaggacgac
     1141 gagcctccgg cccggcctcc cactcctccc ccggccagcg tgagccccca ggcagagccc
     1201 gtgtggaccc cgccagcccc ggctcccgcc gcgcccccct ccaccccggc cgcgcccaag
     1261 cgcaggggct cctcgggctc agtggatgag accctttttg ctcttcctgc tgcatctgag
     1321 cctgtgatac gctcctctgc agaaaatatg gacttgaagg agcagccagg taacactatt
     1381 tcggctggtc aagaggattt cccatctgtc ctgcttgaaa ctgctgcttc tcttccttct
     1441 ctgtctcctc tctcagccgc ttctttcaaa gaacatgaat accttggtaa tttgtcaaca
     1501 gtattaccca ctgaaggaac acttcaagaa aatgtcagtg aagcttctaa agaggtctca
     1561 gagaaggcaa aaactctact catagataga gatttaacag agttttcaga attagaatac
     1621 tcagaaatgg gatcatcgtt cagtgtctct ccaaaagcag aatctgccgt aatagtagca
     1681 aatcctaggg aagaaataat cgtgaaaaat aaagatgaag aagagaagtt agttagtaat
     1741 aacatccttc ataatcaaca agagttacct acagctctta ctaaattggt taaagaggat
     1801 gaagttgtgt cttcagaaaa agcaaaagac agttttaatg aaaagagagt tgcagtggaa
     1861 gctcctatga gggaggaata tgcagacttc aaaccatttg agcgagtatg ggaagtgaaa
     1921 gatagtaagg aagatagtga tatgttggct gctggaggta aaatcgagag caacttggaa
     1981 agtaaagtgg ataaaaaatg ttttgcagat agccttgagc aaactaatca cgaaaaagat
     2041 agtgagagta gtaatgatga tacttctttc cccagtacgc cagaaggtat aaaggatcgt
     2101 tcaggagcat atatcacatg tgctcccttt aacccagcag caactgagag cattgcaaca
     2161 aacatttttc ctttgttagg agatcctact tcagaaaata agaccgatga aaaaaaaata
     2221 gaagaaaaga aggcccaaat agtaacagag aagaatacta gcaccaaaac atcaaaccct
     2281 tttcttgtag cagcacagga ttctgagaca gattatgtca caacagataa tttaacaaag
     2341 gtgactgagg aagtcgtggc aaacatgcct gaaggcctga ctccagattt agtacaggaa
     2401 gcatgtgaaa gtgaattgaa tgaagttact ggtacaaaga ttgcttatga aacaaaaatg
     2461 gacttggttc aaacatcaga agttatgcaa gagtcactct atcctgcagc acagctttgc
     2521 ccatcatttg aagagtcaga agctactcct tcaccagttt tgcctgacat tgttatggaa
     2581 gcaccattga attctgcagt tcctagtgct ggtgcttccg tgatacagcc cagctcatca
     2641 ccattagaag cttcttcagt taattatgaa agcataaaac atgagcctga aaacccccca
     2701 ccatatgaag aggccatgag tgtatcacta aaaaaagtat caggaataaa ggaagaaatt
     2761 aaagagcctg aaaatattaa tgcagctctt caagaaacag aagctcctta tatatctatt
     2821 gcatgtgatt taattaaaga aacaaagctt tctgctgaac cagctccgga tttctctgat
     2881 tattcagaaa tggcaaaagt tgaacagcca gtgcctgatc attctgagct agttgaagat
     2941 tcctcacctg attctgaacc agttgactta tttagtgatg attcaatacc tgacgttcca
     3001 caaaaacaag atgaaactgt gatgcttgtg aaagaaagtc tcactgagac ttcatttgag
     3061 tcaatgatag aatatgaaaa taaggaaaaa ctcagtgctt tgccacctga gggaggaaag
     3121 ccatatttgg aatcttttaa gctcagttta gataacacaa aagataccct gttacctgat
     3181 gaagtttcaa cattgagcaa aaaggagaaa attcctttgc agatggagga gctcagtact
     3241 gcagtttatt caaatgatga cttatttatt tctaaggaag cacagataag agaaactgaa
     3301 acgttttcag attcatctcc aattgaaatt atagatgagt tccctacatt gatcagttct
     3361 aaaactgatt cattttctaa attagccagg gaatatactg acctagaagt atcccacaaa
     3421 agtgaaattg ctaatgcccc ggatggagct gggtcattgc cttgcacaga attgccccat
     3481 gacctttctt tgaagaacat acaacccaaa gttgaagaga aaatcagttt ctcagatgac
     3541 ttttctaaaa atgggtctgc tacatcaaag gtgctcttat tgcctccaga tgtttctgct
     3601 ttggccactc aagcagagat agagagcata gttaaaccca aagttcttgt gaaagaagct
     3661 gagaaaaaac ttccttccga tacagaaaaa gaggacagat caccatctgc tatattttca
     3721 gcagagctga gtaaaacttc agttgttgac ctcctgtact ggagagacat taagaagact
     3781 ggagtggtgt ttggtgccag cctattcctg ctgctttcat tgacagtatt cagcattgtg
     3841 agcgtaacag cctacattgc cttggccctg ctctctgtga ccatcagctt taggatatac
     3901 aagggtgtga tccaagctat ccagaaatca gatgaaggcc acccattcag ggcatatctg
     3961 gaatctgaag ttgctatatc tgaggagttg gttcagaagt acagtaattc tgctcttggt
     4021 catgtgaact gcacgataaa ggaactcagg cgcctcttct tagttgatga tttagttgat
     4081 tctctgaagt ttgcagtgtt gatgtgggta tttacctatg ttggtgcctt gtttaatggt
     4141 ctgacactac tgattttggc tctcatttca ctcttcagtg ttcctgttat ttatgaacgg
     4201 catcaggcac agatagatca ttatctagga cttgcaaata agaatgttaa agatgctatg
     4261 gctaaaatcc aagcaaaaat ccctggattg aagcgcaaag ctgaatgagc ggccgctaag
     4321 taagtaagat ccgcccctct ccctcccccc ccctaacgtt actggccgaa gccgcttgga
     4381 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     4441 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     4501 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     4561 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     4621 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     4681 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     4741 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     4801 ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     4861 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
     4921 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     4981 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     5041 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     5101 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     5161 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     5221 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     5281 gtgaattgct gccctctggt tatgtgtggg agggcggtag tggcgagggc agaggaagtc
     5341 ttctaacatg cggtgacgtg gaggagaatc ccggccctgc tccaaagaag aagcgtaagg
     5401 taaatcgatc gctagtcgag agatcctaat caacctctgg attacaaaat ttgtgaaaga
     5461 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5521 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5581 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5641 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5701 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5761 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5821 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5881 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5941 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6001 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     6061 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6121 agatcacctg caggacaggc gcgccctgct ttttgcttgt actgggtctc tctggttaga
     6181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcacccggg cgattaagga
     6361 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6421 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6601 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6661 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6721 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6781 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     6841 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6901 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6961 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7021 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7081 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7141 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7201 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7261 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7321 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7381 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7441 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7501 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7561 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7621 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7681 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7741 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7801 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7861 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7921 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7981 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8041 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8101 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8161 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8221 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8281 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8341 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8401 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     8461 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8521 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     8581 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8641 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8701 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     8761 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     8821 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     8881 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     8941 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9001 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9061 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     9121 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9181 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9241 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9301 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9361 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9421 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9601 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9661 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9721 cggcgactgc agagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9781 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9841 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9901 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9961 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10021 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10081 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    10141 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10201 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10261 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10321 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10381 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10441 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10501 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10561 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10621 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10681 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10741 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10801 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10861 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10921 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10981 ttcgattagt gaacggatct cgacggtatc gccgaattct caacaatagg cagtattcat
    11041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    11101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    11161 ttttcgggtt tattactcgg acagcagaga tccagtttgg actagtggag ttccgcgtta
    11221 cataacttac gcaaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    11281 caataatgac caatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    11341 tggagtattt acgcaaaact gcccacttgg cagtacatca agtgtatcat ttgccaacaa
    11401 cgccccctat tgacgtcaat gacgcaaaat ggcccgcctg gcattatgcc cagtacatga
    11461 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    11521 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
    11581 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
    11641 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
    11701 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
    11761 cacgctgttt tgacctccat tcaagacacc ggcggcctta attaaacc
//
