LOCUS       sequence-171274-        8937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent human protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 GFP-Rtn4HD
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    join(1463..2036,2037..2039,2040..2048)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1629..1646)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1856..1875
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2049..2444
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2454..2507
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2511..2531
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2557..3145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2610..2630)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3028..3039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3293..3473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3520..3538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3606..3710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3711..3779,3780..4571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3929..3948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4742..5330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5484..5501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5741..5762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5777..5782,5783..5800,5801..5807)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5815..5831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5820..5842
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5839..5855
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5839..5855
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5905..5924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6116..6749
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6796..6921
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7418..7651
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7836..7880
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8176..8293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8345..8648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8649..8852
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8803..8823
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8849..8873
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
       61 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
      181 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
      241 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
      301 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
      361 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
      421 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
      541 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
      601 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
      661 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtcc
      721 ggactcagat ctcgagcaca agcagagata gagagcatag ttaaacccaa agttcttgtg
      781 aaagaagctg agaaaaaact tccttccgat acagaaaaag aggacagatc accatctgct
      841 atattttcag cagagctgag taaaacttca gttgttgacc tcctgtactg gagagacatt
      901 aagaagactg gagtggtgtt tggtgccagc ctattcctgc tgctttcatt gacagtattc
      961 agcattgtga gcgtaacagc ctacattgcc ttggccctgc tctctgtgac catcagcttt
     1021 aggatataca agggtgtgat ccaagctatc cagaaatcag atgaaggcca cccattcagg
     1081 gcatatctgg aatctgaagt tgctatatct gaggagttgg ttcagaagta cagtaattct
     1141 gctcttggtc atgtgaactg cacgataaag gaactcaggc gcctcttctt agttgatgat
     1201 ttagttgatt ctctgaagtt tgcagtgttg atgtgggtat ttacctatgt tggtgccttg
     1261 tttaatggtc tgacactact gattttggct ctcatttcac tcttcagtgt tcctgttatt
     1321 tatgaacggc atcaggcaca gatagatcat tatctaggac ttgcaaataa gaatgttaaa
     1381 gatgctatgg ctaaaatcca agcaaaaatc cctggattga agcgcaaagc tgaatgagcg
     1441 gccgctaagt aagtaagatc cgcccctctc cctccccccc cctaacgtta ctggccgaag
     1501 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     1561 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     1621 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     1681 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     1741 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     1801 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     1861 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     1921 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     1981 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     2041 ccacaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     2101 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     2161 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     2221 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     2281 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     2341 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     2401 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcggtagt ggcgagggca
     2461 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgct ccaaagaaga
     2521 agcgtaaggt aaatcgatcg ctagtcgaga gatcctaatc aacctctgga ttacaaaatt
     2581 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2641 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2701 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2761 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2821 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2881 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2941 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3001 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3061 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3121 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     3181 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3241 acgaagacaa gatcacctgc aggacaggcg cgccctgctt tttgcttgta ctgggtctct
     3301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcacccgggc
     3481 gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     3541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     3961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4861 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
     5581 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5641 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
     5701 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5761 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5821 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
     5881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5941 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     6001 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     6061 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     6121 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     6181 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     6241 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     6301 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     6361 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     6421 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     6481 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     6541 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     6601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6721 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6781 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6841 ggcgaggggc ggcgactgca gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6901 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6961 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7021 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7081 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7141 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7201 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7261 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     7321 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     7381 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     7441 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     7501 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     7561 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     7621 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     7681 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     7741 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     7801 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     7861 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     7921 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7981 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     8041 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     8101 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattctc aacaataggc
     8161 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8221 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     8281 aattcaaaat tttcgggttt attactcgga cagcagagat ccagtttgga ctagtggagt
     8341 tccgcgttac ataacttacg caaaatggcc cgcctggctg accgcccaac gacccccgcc
     8401 cattgacgtc aataatgacc aatgttccca tagtaacgcc aatagggact ttccattgac
     8461 gtcaatgggt ggagtattta cgcaaaactg cccacttggc agtacatcaa gtgtatcatt
     8521 tgccaacaac gccccctatt gacgtcaatg acgcaaaatg gcccgcctgg cattatgccc
     8581 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     8641 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     8701 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     8761 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     8821 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     8881 gacgccatcc acgctgtttt gacctccatt caagacaccg gcggccttaa ttaaacc
//
