LOCUS       sequence-171276-        9906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 mCherry-Atl1
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(2432..3005,3006..3008,3009..3017)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2598..2615)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2825..2844
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3018..3413
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3423..3476
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3480..3500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3526..4114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3579..3599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3997..4008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4262..4442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4489..4507)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4575..4679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4680..4748,4749..5540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4898..4917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5711..6299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6453..6470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6710..6731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6746..6751,6752..6769,6770..6776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6784..6800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6789..6811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6808..6824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6808..6824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6874..6893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7085..7718
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7765..7890
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8387..8620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8805..8849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9145..9262
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9314..9617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9618..9821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9772..9792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9818..9842
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      721 tctcgagcta tggccaagaa ccgcagggac agaaacagtt ggggtggatt ttcggaaaag
      781 acatatgaat ggagctcaga agaggaggag ccagtgaaaa aggcaggacc agtccaagtc
      841 ctcattgtca aagatgacca ttcctttgag ttagatgaaa ctgcattaaa tcggatcctt
      901 ctctcggagg ctgtcagaga caaggaggtt gttgctgtat ctgttgctgg agcatttaga
      961 aaaggaaaat cattcctgat ggacttcatg ttgagataca tgtacaacca ggaatcagtt
     1021 gattgggttg gagactacaa tgaaccattg actggttttt catggagagg tggatctgaa
     1081 cgagagacca caggaattca gatatggagt gaaatcttcc ttatcaataa acctgatggt
     1141 aaaaaggttg cagtgttatt gatggatact cagggaacct ttgatagtca gtcaactttg
     1201 agagattcag ccacagtatt tgcccttagc acaatgatca gctcaataca ggtatataac
     1261 ttatcccaaa atgtccagga ggatgatctt cagcacctcc agcttttcac tgagtatggc
     1321 agactggcaa tggaggaaac attcctgaag ccatttcaga gtctgatatt tcttgttcga
     1381 gactggagtt tcccatacga attttcatat ggagccgatg gtggtgccaa attcttggaa
     1441 aaacgcctca aggtctcagg gaaccagcat gaagaactac agaacgtcag aaaacacatc
     1501 cattcctgtt tcaccaacat ttcctgtttt ctgctacctc atcctggctt aaaagtagct
     1561 accaatccaa actttgatgg aaaattgaaa gaaatagatg atgaattcat caaaaacttg
     1621 aaaatactga ttccttggct acttagtccc gagagcctag atattaaaga gatcaatggg
     1681 aataaaatca cctgccgggg tctggtggag tacttcaagg cttatataaa gatctatcaa
     1741 ggtgaagaat taccacatcc caaatccatg ttacaggcca cagcagaagc taacaattta
     1801 gcagccgtgg caactgccaa ggacacatac aacaaaaaaa tggaagagat ttgtggtggt
     1861 gacaaaccat ttctggcccc aaatgacttg cagaccaaac acctgcaact taaggaagaa
     1921 tctgtgaagc tattccgagg ggtgaagaag atgggtgggg aagaatttag ccggcgttac
     1981 ctgcagcagt tggagagtga aatagatgaa ctttacatcc aatatatcaa gcacaatgat
     2041 agcaaaaata tcttccatgc agctcgtacc ccagccacac tgtttgtagt catctttatc
     2101 acatatgtga ttgctggtgt gactggattc attggtttgg acatcatagc tagcctatgc
     2161 aatatgataa tgggactgac ccttatcacc ctgtgcactt gggcatatat ccggtactct
     2221 ggagaatacc gagagctggg agctgtaata gaccaggtgg ctgcagctct gtgggaccag
     2281 ggaagtacaa atgaggcttt gtacaagctt tacagtgcag cagcaaccca cagacatctg
     2341 tatcatcaag ctttccctac accaaagtcg gaatctactg aacaatcaga aaagaaaaaa
     2401 atgtaagcgg ccgctaagta agtaagatcc gcccctctcc ctcccccccc ctaacgttac
     2461 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     2521 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     2581 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     2641 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     2701 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     2761 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     2821 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     2881 ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt
     2941 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     3001 ataatatggc cacaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     3061 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     3121 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     3181 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     3241 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     3301 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     3361 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcggtagtg
     3421 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctgctc
     3481 caaagaagaa gcgtaaggta aatcgatcgc tagtcgagag atcctaatca acctctggat
     3541 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3601 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3661 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3721 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3781 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3841 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3901 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3961 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4021 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4081 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     4141 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4201 tcactcccaa cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac
     4261 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4321 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4381 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4441 cacccgggcg attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     4501 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4921 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6481 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     6541 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6601 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     6661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6781 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
     6841 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6901 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     6961 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     7021 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     7081 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     7141 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7201 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7261 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7321 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7381 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7441 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7501 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7681 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7741 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7801 acggcaagag gcgaggggcg gcgactgcag agtacgccaa aaattttgac tagcggaggc
     7861 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7921 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7981 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8041 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8101 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8161 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8221 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     8281 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     8341 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8401 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8461 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8521 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8581 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8641 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8701 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8761 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8821 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8881 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8941 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     9001 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     9061 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattctca
     9121 acaataggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9181 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9241 aattacaaaa attcaaaatt ttcgggttta ttactcggac agcagagatc cagtttggac
     9301 tagtggagtt ccgcgttaca taacttacgc aaaatggccc gcctggctga ccgcccaacg
     9361 acccccgccc attgacgtca ataatgacca atgttcccat agtaacgcca atagggactt
     9421 tccattgacg tcaatgggtg gagtatttac gcaaaactgc ccacttggca gtacatcaag
     9481 tgtatcattt gccaacaacg ccccctattg acgtcaatga cgcaaaatgg cccgcctggc
     9541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9781 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     9841 tcgcctggag acgccatcca cgctgttttg acctccattc aagacaccgg cggccttaat
     9901 taaacc
//
