LOCUS       sequence-171290-        9915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 GFP-Atl1
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    join(2441..3014,3015..3017,3018..3026)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2607..2624)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2834..2853
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3027..3422
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3432..3485
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3489..3509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3535..4123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3588..3608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4006..4017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4271..4451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4498..4516)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4584..4688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4689..4757,4758..5549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4907..4926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5720..6308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6462..6479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6719..6740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6755..6760,6761..6778,6779..6785)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6793..6809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6798..6820
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6817..6833
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6817..6833
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6883..6902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7094..7727
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7774..7899
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8396..8629
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8814..8858
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9154..9271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9323..9626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9627..9830
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9781..9801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9827..9851
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
       61 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
      181 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
      241 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
      301 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
      361 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
      421 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
      541 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
      601 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
      661 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtcc
      721 ggactcagat ctcgagctat ggccaagaac cgcagggaca gaaacagttg gggtggattt
      781 tcggaaaaga catatgaatg gagctcagaa gaggaggagc cagtgaaaaa ggcaggacca
      841 gtccaagtcc tcattgtcaa agatgaccat tcctttgagt tagatgaaac tgcattaaat
      901 cggatccttc tctcggaggc tgtcagagac aaggaggttg ttgctgtatc tgttgctgga
      961 gcatttagaa aaggaaaatc attcctgatg gacttcatgt tgagatacat gtacaaccag
     1021 gaatcagttg attgggttgg agactacaat gaaccattga ctggtttttc atggagaggt
     1081 ggatctgaac gagagaccac aggaattcag atatggagtg aaatcttcct tatcaataaa
     1141 cctgatggta aaaaggttgc agtgttattg atggatactc agggaacctt tgatagtcag
     1201 tcaactttga gagattcagc cacagtattt gcccttagca caatgatcag ctcaatacag
     1261 gtatataact tatcccaaaa tgtccaggag gatgatcttc agcacctcca gcttttcact
     1321 gagtatggca gactggcaat ggaggaaaca ttcctgaagc catttcagag tctgatattt
     1381 cttgttcgag actggagttt cccatacgaa ttttcatatg gagccgatgg tggtgccaaa
     1441 ttcttggaaa aacgcctcaa ggtctcaggg aaccagcatg aagaactaca gaacgtcaga
     1501 aaacacatcc attcctgttt caccaacatt tcctgttttc tgctacctca tcctggctta
     1561 aaagtagcta ccaatccaaa ctttgatgga aaattgaaag aaatagatga tgaattcatc
     1621 aaaaacttga aaatactgat tccttggcta cttagtcccg agagcctaga tattaaagag
     1681 atcaatggga ataaaatcac ctgccggggt ctggtggagt acttcaaggc ttatataaag
     1741 atctatcaag gtgaagaatt accacatccc aaatccatgt tacaggccac agcagaagct
     1801 aacaatttag cagccgtggc aactgccaag gacacataca acaaaaaaat ggaagagatt
     1861 tgtggtggtg acaaaccatt tctggcccca aatgacttgc agaccaaaca cctgcaactt
     1921 aaggaagaat ctgtgaagct attccgaggg gtgaagaaga tgggtgggga agaatttagc
     1981 cggcgttacc tgcagcagtt ggagagtgaa atagatgaac tttacatcca atatatcaag
     2041 cacaatgata gcaaaaatat cttccatgca gctcgtaccc cagccacact gtttgtagtc
     2101 atctttatca catatgtgat tgctggtgtg actggattca ttggtttgga catcatagct
     2161 agcctatgca atatgataat gggactgacc cttatcaccc tgtgcacttg ggcatatatc
     2221 cggtactctg gagaataccg agagctggga gctgtaatag accaggtggc tgcagctctg
     2281 tgggaccagg gaagtacaaa tgaggctttg tacaagcttt acagtgcagc agcaacccac
     2341 agacatctgt atcatcaagc tttccctaca ccaaagtcgg aatctactga acaatcagaa
     2401 aagaaaaaaa tgtaagcggc cgctaagtaa gtaagatccg cccctctccc tccccccccc
     2461 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     2521 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     2581 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     2641 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     2701 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     2761 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     2821 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     2881 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     2941 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     3001 aacacgatga taatatggcc acaaccatgg ccaagccttt gtctcaagaa gaatccaccc
     3061 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     3121 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     3181 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     3241 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     3301 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     3361 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     3421 gcggtagtgg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     3481 gccctgctcc aaagaagaag cgtaaggtaa atcgatcgct agtcgagaga tcctaatcaa
     3541 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3601 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3661 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3721 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3781 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3841 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3901 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3961 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4021 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4081 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     4141 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4201 agggctaatt cactcccaac gaagacaaga tcacctgcag gacaggcgcg ccctgctttt
     4261 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4441 aatctctagc acccgggcga ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     4501 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4681 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4741 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4801 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4861 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4921 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4981 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5041 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5101 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5161 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5221 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5281 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5341 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5401 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5461 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5521 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5581 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5641 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6421 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6481 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6541 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6661 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6721 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6781 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6841 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6901 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     6961 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     7021 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     7081 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     7141 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7201 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7261 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7321 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7381 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7441 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7501 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7741 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7801 gaagcgcgca cggcaagagg cgaggggcgg cgactgcaga gtacgccaaa aattttgact
     7861 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7921 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7981 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8041 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8101 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8161 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8221 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     8281 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8341 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8401 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8461 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8521 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8581 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8641 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8701 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8761 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8821 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8881 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8941 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9001 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9061 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     9121 gaattctcaa caataggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tactcggaca gcagagatcc
     9301 agtttggact agtggagttc cgcgttacat aacttacgca aaatggcccg cctggctgac
     9361 cgcccaacga cccccgccca ttgacgtcaa taatgaccaa tgttcccata gtaacgccaa
     9421 tagggacttt ccattgacgt caatgggtgg agtatttacg caaaactgcc cacttggcag
     9481 tacatcaagt gtatcatttg ccaacaacgc cccctattga cgtcaatgac gcaaaatggc
     9541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     9661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     9721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     9781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     9841 accgtcagat cgcctggaga cgccatccac gctgttttga cctccattca agacaccggc
     9901 ggccttaatt aaacc
//
