LOCUS       sequence-171291-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 mCherry-Atl3
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(2381..2954,2955..2957,2958..2966)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2547..2564)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2774..2793
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2967..3362
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3372..3425
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3429..3449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3475..4063
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3528..3548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3946..3957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4211..4391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4438..4456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4524..4628
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4629..4697,4698..5489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4847..4866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5660..6248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6402..6419
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6659..6680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6695..6700,6701..6718,6719..6725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6733..6749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6738..6760
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6757..6773
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6757..6773
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6823..6842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7034..7667
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7714..7839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8336..8569
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8754..8798
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9094..9211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9263..9566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9567..9770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9721..9741
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9767..9791
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 attcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      721 tctcgagcta tgttgtcccc tcagcgagtg gcagcagctg cctcaagagg agcagatgat
      781 gccatggaga gcagcaagcc tggtccagtg caggttgttt tggttcagaa agatcaacat
      841 tcctttgagc tagatgagaa agccttggcc agcatcctct tgcaggacca catccgagat
      901 cttgatgtgg tggtggtttc agtggctggt gccttccgaa agggcaagtc cttcattctg
      961 gattttatgc tacgatactt atattctcag aaggaaagtg gccattcaaa ttggttgggt
     1021 gacccagaag aaccgttaac aggattttcc tggagagggg gatctgatcc agaaaccact
     1081 gggattcaaa tctggagtga agttttcact gtggagaagc caggtgggaa gaaggttgca
     1141 gttgttctga tggataccca gggggcattt gacagccagt caactgtgaa agactgtgct
     1201 accatctttg ctctaagcac tatgactagt tctgttcaga tttataattt atctcagaac
     1261 attcaagaag atgatcttca acagctgcag ctcttcacag aatacggtcg tctggcaatg
     1321 gatgaaattt tccaaaagcc tttccagaca ctgatgtttt tggttagaga ttggagtttc
     1381 ccttatgaat atagctatgg actccaagga ggaatggcat ttttggataa gcgtttacag
     1441 gtgaaggaac atcaacatga agaaattcag aatgttcgaa atcacattca ctcatgtttc
     1501 tccgatgtca cctgctttct cttaccacat ccaggactcc aggtggccac aagccctgac
     1561 tttgatggga aattaaaaga tattgctggt gaattcaaag agcagttaca ggcactgata
     1621 ccgtatgtat taaacccatc taagttaatg gaaaaggaga tcaatggctc aaaggtcacc
     1681 tgtcggggac tactggagta ttttaaggca tatattaaaa tttatcaagg agaagatctg
     1741 cctcacccca agtccatgct tcaggccact gctgaagcca acaacttagc agctgcagcc
     1801 tctgccaagg acatttatta taacaacatg gaagaggttt gtgggggaga gaaaccttat
     1861 ttgtctccag acattctaga ggagaagcac tgtgaattca aacaacttgc tctggaccat
     1921 tttaagaaga ccaagaagat gggtgggaag gatttcagct ttcgttacca gcaggagctg
     1981 gaggaggaaa tcaaggaatt atatgagaac ttctgcaagc acaatggtag caagaacgtc
     2041 ttcagcacct tccgaacccc tgcagtgctg ttcacgggca ttgtagcttt gtacatagcc
     2101 tcaggcctca ctggcttcat aggtcttgag gttgtagccc agttgttcaa ctgtatggtt
     2161 ggactactgt taatagcact cctcacctgg ggctacatca ggtattctgg tcaatatcgt
     2221 gagctgggcg gagctattga ttttggtgcc gcatatgtgt tggagcaggc ttcttctcat
     2281 atcggtaatt ccactcaggc cactgtgagg gatgcagttg ttggaagacc atccatggat
     2341 aaaaaagctc aataagcggc cgctaagtaa gtaagatccg cccctctccc tccccccccc
     2401 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     2461 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     2521 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     2581 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     2641 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     2701 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     2761 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     2821 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     2881 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     2941 aacacgatga taatatggcc acaaccatgg ccaagccttt gtctcaagaa gaatccaccc
     3001 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     3061 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     3121 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     3181 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     3241 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     3301 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     3361 gcggtagtgg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     3421 gccctgctcc aaagaagaag cgtaaggtaa atcgatcgct agtcgagaga tcctaatcaa
     3481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     4081 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4141 agggctaatt cactcccaac gaagacaaga tcacctgcag gacaggcgcg ccctgctttt
     4201 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4261 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4321 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4381 aatctctagc acccgggcga ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     4441 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4501 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4561 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4621 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4681 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4741 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4801 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4861 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4921 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4981 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5041 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5101 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5161 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5221 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5281 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5341 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5401 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5461 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5521 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5581 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6301 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6361 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6421 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6481 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6541 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6601 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6661 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6721 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6781 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6841 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     6901 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     6961 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     7021 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     7081 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7141 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7201 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7261 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7321 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7381 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7441 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7501 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7561 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7621 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7681 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7741 gaagcgcgca cggcaagagg cgaggggcgg cgactgcaga gtacgccaaa aattttgact
     7801 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7861 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7921 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7981 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8041 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8101 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8161 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     8221 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8281 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8341 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8401 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8461 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8521 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8581 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8641 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8701 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8761 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8821 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8881 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8941 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9001 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     9061 gaattctcaa caataggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9121 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9181 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tactcggaca gcagagatcc
     9241 agtttggact agtggagttc cgcgttacat aacttacgca aaatggcccg cctggctgac
     9301 cgcccaacga cccccgccca ttgacgtcaa taatgaccaa tgttcccata gtaacgccaa
     9361 tagggacttt ccattgacgt caatgggtgg agtatttacg caaaactgcc cacttggcag
     9421 tacatcaagt gtatcatttg ccaacaacgc cccctattga cgtcaatgac gcaaaatggc
     9481 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9541 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     9601 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     9661 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     9721 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     9781 accgtcagat cgcctggaga cgccatccac gctgttttga cctccattca agacaccggc
     9841 ggccttaatt aaacc
//
