LOCUS       sequence-171299-        8334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PLXNB2 full-length human cDNA CDS in gateway entry plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, 
            Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, 
            Dai G, Zou H, Friedel RH
  TITLE     Plexin-B2 facilitates glioblastoma infiltration by modulating cell 
            biomechanics.
  JOURNAL   Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
  PUBMED    33514835
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-01-29"; volume: "4(1):145. doi"; pages: " 
            10.1038/s42003-021-01667-4"
COMMENT     Sequence Label: pENTR223-PLXNB2
FEATURES             Location/Qualifiers
     source          1..8334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      113..140
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(299..316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(470..1058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(550..569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1151..1942)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2373..2389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2373..2389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2394..2413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2394..2412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2430..2529
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(8074..8173)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8189..8206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8189..8205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8198..8220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      join(8269..8334,1..21)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
       61 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
      121 atcctgacgg atggcctttt tgcgtttcta caaactcttc ctggctagcg gtacgcgtat
      181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1081 cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt ggtcatgggt
     1141 ggctcgaggg ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     1201 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     1261 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     1321 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     1381 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     1441 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     1501 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     1561 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     1621 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     1681 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     1741 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     1801 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     1861 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     1921 cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg
     1981 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     2041 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
     2101 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     2161 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct
     2221 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat
     2281 ttctgtcctg gctggtctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac
     2341 atcagagatt ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg
     2401 actcactata ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga
     2461 cctgttcgtt gcaacaaatt gatgagcaat tattttttat aatgccaact ttgtacaaga
     2521 aagctgggcc catgaggccc tagaggtcag tgaccttgtt ctccagtgca gcggcaatct
     2581 gctgcaggcg gaaggccagc tgcatcttct gggcggcagg atcctcctcc aaggcattga
     2641 tgatctcgtc atagtacttc tgcgtgtatt ggtagagctg gtggagtgcc acgagggtgt
     2701 tcaaggagtc cgtgtgcgcc cgggaaatct ctgccaggtg tgtgttcatg tcctggtcgc
     2761 tgacctgcac catctgccgg atccccttgt agtaatcctc caccatcttc ttgtaggtgg
     2821 agatctcctt ggcgtacagc agcttgttgc tgggagaatc gcggctcagc ttatgctccg
     2881 tgcgcgtgca ggcatccatg aaggtctgcg cgatgactga cagcgaggcg tccaccacct
     2941 cgtggacatg cacgtcaaag atgaagtggg ggttcttgag gatgttcacc cagaaccgga
     3001 gcggtaagct gttcgtcttc cagatgtgga tggtgtcttc atcctggatg ttgtgcttct
     3061 ctgcctgctc gtccaggaag tcgaagaagt acttgactgc aggtggcacc gcgtgcccag
     3121 gcgccagcac gctctggaag aagttgtcca caaactgctg cagtgtgccc ttgactgaga
     3181 gcagccgcgt caggtagatc tcggtgatgg ccttcgtccg ctccttctct ttcacgctgc
     3241 ctctcttgga cttgccctcg tccacctcgt cggtcggccg caccaggtgc cacacccggt
     3301 tctcctcctc caggagggca tggcgctccc caggcaggtc ctgctggctg tcctccggct
     3361 gctgggagac ccccaccttg gacaggatga gggtggctcc atcccggaca ttgtagtgca
     3421 taagggtgtt gacgcgcttc caccggccct cccgctgtga cgtcaggtcc aggtccgaca
     3481 ggatctgcgc tgtggagccc ggacgccact ccaggaccac gctgtctggc ctgggccagc
     3541 aggagcaggg ctgcccacgg tacacctggt caatgatctt ctccttgacc tgggagatgg
     3601 tgtcacagtt gaggaccttc accgggatgg cgtccactcc ctcgtcctgc acgatcacgc
     3661 tcaccgtcag gggtgcgtac tccacatcat cccccagcag ccccgtgtcg ttgagagtgt
     3721 acttggcctt cttctgtacc gcatccaccg ggcccttttc cacctgatgt ttgatggcct
     3781 tgaagagctt gtacaggggc tccccggcac tgtccttgag gtactggtac aggcagatgg
     3841 acatccagtt ggacagcatc ctctccacca cagtctcaga cctgcgcagc atcagcttgg
     3901 ggttcttggc caccacgtac tgctccagga gctccaggaa gagcgtgtgc atgatgtccg
     3961 tgtagtactc cagtttcccg tgcagcgcca ccgtcagcag ggacgcgaag tagaccttgg
     4021 cgcgggccga gaactcccgc tggttctcca gggtgtggat gaaattgatg aggaaagact
     4081 tgctgttcag caggttggag aactggtaga gggcctgctc caccaccggc cgccgcggct
     4141 cagggatgtc cagcttgccg gtgatcatca cgtccttgtc gccgtccttg gagggcagga
     4201 agaagacgcg gtcggtgtag gtcttgtagt ccagcacggg gatgccggcc tcgtgcacgt
     4261 cgttggtctg gtcctccatc tcgatcatca ggtctgtgaa ttccttcttg cagcggtccc
     4321 gcacgctctc ctccaggccc tccagctggg acttgatctt ctcatactct cgttcggcct
     4381 gctggctctt cctccagtag cagtagacag acaccgcgat gacgaccacc atgggcacga
     4441 tgaccagcgg caagatgagg ctgagcggca cgtcgctcac ccgtgtgtcg tactccacgc
     4501 ggcccagcac ccactcgcga gagccgaact tcacaatgaa ctcgggcagg ttgtgtgtgg
     4561 tgtctcgttt ctgccgccgc ttgggcgggg gctgcacctc cgggggctca cagtacaggt
     4621 cggtctccgt cagcgtcttc atggtgcagc gctcggcacc cacgaaggcc tcggcctcct
     4681 gcagcgtcat cgccttgttc agattggtgc cccgggcgtg gatgagcttg ttgacctgct
     4741 tcttgacgcc acctgtgaag ttctcaaagg tggggtcagg cacgtactcg aaggccccgg
     4801 cctctgttct gagcagggca cggtgcccgt ccatctcgat cagcaccgtg aggttgtagg
     4861 cctctggctc ctcaggcaca gccggggaca ggaagacgac cttggtgtca ttgtggaaca
     4921 cgtagtctgt acccaccacc gtcatgggct gcagggattc agcctcccgc ggcggctgcc
     4981 aggactgcag gggctccgcg atgaccacca tggcaaacct ctggatcagg ctgaagccct
     5041 gacccgtgac gttgatgctg cggccaccac tggcaaagct tcgtagcggc tcgaaggctc
     5101 gcagtacggg gttttcgcgg taggtgaaga agatgccggg gttgggcacg ggggaccccc
     5161 cgtaggagac ctccagaagc atctggcccc gtgtcgcctg ggggccagtg acacactgga
     5221 gctgcgcccc aaacttcgtc actttacacg ggacgccgtt gagggtcacc cgcacgtcct
     5281 cctgggagcc cgtgtccagg tgggtgccgt ggatggtcag tgtggtgccg cccgcctgcg
     5341 gtccctgctg cggctccaca ctgagaggct tgggctgttg gaaggtgaac tggacattgg
     5401 gaggcgaacg gcccagtttc ccgaagacgt ccacctcgac accccccgtg aaaggcgtct
     5461 ccgcagcctc gatcacacac acgatccggg tggacacgga gtaacgttcc ggctgaaagg
     5521 agcagttccg gccggccaca gagatcctct ggatgtcccc tgcttggacg cccaaattgg
     5581 accccaggat ggtgatgcgg atgcccccac ccagggggcc cgtctcaggc tggatcctgg
     5641 tgatgacggg cggcgggcac tcggaggtgg tgttgcacag ggcctcatac acgcacctgc
     5701 tctggccccc gcaccacgca cacctgtagt cggggttagc ggcccggcac aggctgcagt
     5761 cgctgcggcc aaaggagcag ttgtagaggg tcacatggag cttgctgtcg atattcttgc
     5821 cgtaagactt gacgtagagg tgcaggggca gcgtctcgtt ggcatcgtgg gacagctttg
     5881 gggtccgaaa ggcgaaggtc ccagattcct gcatggtcac cggctccatg aacttgagca
     5941 agtcactgcc cacgtgcagg gaggaaccct tcacggtgtc caggttcttg ccctggaagt
     6001 tcacatctgt ctcgtggttc atggggatca ccagggggct gggtcccagg aactggggac
     6061 agctgtcctc catgtgggca cggacgatgc cgtcctcagg gttgggcgaa gcctcccggc
     6121 actcgtggta gcgcaggtcc cactggcagg tccagcggtt gctcacgcag gagatgcacg
     6181 gcaggttctc ctccaggctc atggcctggc ggcagtcgta gaaggggtac tggtaggacg
     6241 tgaggaagat gttgcctcgt ctaaggagga gctggatggt cacggccacg tggtcctggc
     6301 ctggcggtgt gacggggatg ctgcttgggg agttgcagat gacggcctcg ccctccacgc
     6361 gggcggggtg tggcggcgac tccccaaaaa ggcacagcaa ctcgtcctcc tcgctcaggg
     6421 cagggagggg gctgacggtc agctgcacct ccccctgggc ccgccggctc atgttctgtg
     6481 gctgggcgct ggtgacggcc acgcaggact tgcttcggct ccacagccag tggctggcct
     6541 cctcggcccg cggacactcg gccttccggg tgcatcgtcc ctcgacgacg caccagccgc
     6601 agtaggggtc ctgggagtcg cggcactggg tgcaggtcgg gtagctcagg cactcctgca
     6661 ccggcagccg gaacaccttg tcctgggtca tggcgtacag gctgcccagg tctccagaca
     6721 gtaccaggtc gcgcttgact ctcttgttta tctccacaag gatagagtcg tactctgagg
     6781 aggtgccatc tggggtgagg tacaccttga ggatccggcc atcagaggtg cccagaaaag
     6841 caacagtgtg gttgttctcg gcggcgaccg tcacggccgt gaggttcagg cctccacgct
     6901 gcagcacggc tgtgcctctg agcccgtcgc ggctgcccag cgggtagggc aggtgctccg
     6961 agccacatgg gaagctcttg ctggagcccg gcgcgtggcc gccgcactgg atatcgccgt
     7021 ggaagggctt gtagaagatg tcacgggcct cccgggtgcc tgtgtaacag gcgttgcggt
     7081 tggcctccat cttggcgtgc accttgtcca gcgggaacag gcagaggccc gcaccgggcc
     7141 ccccactgct ccggctgtct ctgctgaaga cagcatatag caccctgcca gagccaggcg
     7201 cagccacgga ggcggccagg caggtgccaa aggcagcggc gtggatgtcg gggtcccggc
     7261 actgcaggtc catctccagg taggagtagt agttggggtc ttctctgcac atgcgtgcca
     7321 gcagcgtgcg gttccgggcc gggtgcttgt cctgctggtt gaagacaaag aagacgtagg
     7381 ggccgtcctc gaaggccgcc acgaactgct gtgtgttggt ggacaggtag ccggccttgt
     7441 aggtggcgtg gtccgtgtag gcttcaaagg cctccctgct gtcagtccgg tccaacagcc
     7501 gagtgctcac gatgatgccg ttgtcgtgtg gcccattgcc tttgcccaca aacagcacgc
     7561 ggtcaccacc aggacccgtg gagctcacca gccccactgt ggccacgccc tcatcattgc
     7621 tggccacgaa agacttctcc ccgctgccgt cctcgtagaa caggcggagg gagatgttgc
     7681 tcagggcgcg cagagcgcag atgcccttga agaggctgcc gcactccacc aggcgcttcc
     7741 tgggagggtc gagcagcagc agctggttga cattgtcagt catctcagcc tcatggcact
     7801 ggctggcctc gatgggcggc gtgcacttct tgttgtccag ggccgggccc gtggccacct
     7861 gctgctccag ctgcagcttc gcatccagct ggtagagggc attcaccgcc cccaggtaca
     7921 ccacgcctga ggcctcatcc acagccaggt ggttcagctc tttctcgctg cggaagaagt
     7981 ccagcttgcg gggcctcagg ctggcacctg cgcccagcag gcccagcagg gtcagggccc
     8041 agagctgcag tgccatggtg ggccttgacg gcccttctgc ttttttgtac aaagttggca
     8101 ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     8161 aaatcattat ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg
     8221 aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga
     8281 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaac
//
