LOCUS       sequence-17130-e        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Kumar S, Kao WH, Howley PM
  TITLE     Physical interaction between specific E2 and Hect E3 enzymes 
            determines functional cooperativity.
  JOURNAL   J Biol Chem 1997 May 23;272(21):13548-54.
  PUBMED    9153201
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "1997-05-23"; volume: "272(21)"; pages: "13548-54"
COMMENT     Sequence Label: p3990 pET ubcH8
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..78
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             592..609
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(662..680)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      676..723
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(847..866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggnttccncn gaatattttg tttactttaa gaaggagata tacatatggc tagcatgact
       61 ggtggacagc aaatgggtcg cggatccccc aacatggcga gcatgcgagt ggtgaaggag
      121 ctggaggatc ttcagaagaa gcctccccca tacctgcgga acctgtccag cgatgatgcc
      181 aatgtcctgg tgtggcacgc tctcctccta cccgaccaac ctccctacca cctgaaagcc
      241 ttcaacctgc gcatcagctt cccgccggag tatccgttca agcctcccat gatcaaattc
      301 acaaccaaga tctaccaccc caacgtggac gagaacggac agatttgcct gcccatcatc
      361 agcagtgaga actggaagcc ttgcaccaag acttgccaag tcctggaggc cctcaatgtg
      421 ctggtgaata gaccgaatat cagggagccc ctgcggatgg acctcgctga cctgctgaca
      481 cagaatccgg agctgttcag aaagaatgcc gaagagttca ccctccgatt cggagtggac
      541 cggccctccc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      601 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      661 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      721 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgcttttacg gcacctcgac
      961 cccaaaaaac ttgatnangg tgatggttca cgtantgggc catcgccctt gatagacggt
     1021 tttncncctt tgacgttgga gtcccgttct ttaatagtgg actcttgttc caactggaac
     1081 aacactcanc ctttctcggt ctttctttga tttntangga tttngcnnat tcggcntntt
     1141 gnttaaaant gagctgattt aacaaaattt acncganttt aaaaaantta nncttnnant
     1201 taggggnctt ttnggnaatg ggcggaaccc
//
