LOCUS       sequence-171306-        1518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pLX302-MKP5-V5 puro
FEATURES             Location/Qualifiers
     source          1..1518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1448..1472)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1474..1515
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgcctccgt ctcctttaga cgacagggta gtagtggcac tatctaggcc cgtccgacct
       61 caggatctca acctttgttt agactctagt taccttggct ctgccaaccc aggcagtaac
      121 agccaccctc ctgtcatcgc caccaccgtt gtgtccctca aggctgcgaa tctgacgtat
      181 atgccctcat ccagcggctc tgcccgctcg ctgaattgtg gatgcagcag tgccagctgc
      241 tgcactgtgg caacctacga caaggacaat caggcccaaa cccaagccat tgccgctggc
      301 accaccacca ctgccatcgg aacctctacc acctgccctg ctaaccagat ggtcaacaat
      361 aatgagaata caggctctct aagtccatca agtggggtgg gcagccctgt gtcagggacc
      421 cccaagcagc tagccagcat caaaataatc taccccaatg acttggcaaa gaagatgacc
      481 aaatgcagca agagtcacct gccgagtcag ggccctgtca tcattgactg caggcccttc
      541 atggagtaca acaagagtca catccaagga gctgtccaca ttaactgtgc cgataagatc
      601 agccggcgga gactgcagca gggcaagatc actgtcctag acttgatttc ctgtagggaa
      661 ggcaaggact ctttcaagag gatcttttcc aaagaaatta tagtttatga tgagaatacc
      721 aatgaaccaa gccgagtgat gccctcccag ccacttcaca tagtcctcga gtccctgaag
      781 agagaaggca aagaacctct ggtgttgaaa ggtggactta gtagttttaa gcagaaccat
      841 gaaaacctct gtgacaactc cctccagctc caagagtgcc gggaggtggg gggcggcgca
      901 tccgcggcct cgagcttgct acctcagccc atccccacca cccctgacat cgagaacgct
      961 gagctcaccc ccatcttgcc cttcctgttc cttggcaatg agcaggatgc tcaggacctg
     1021 gacaccatgc agcggctgaa catcggctac gtcatcaacg tcaccactca tcttcccctc
     1081 taccactatg agaaaggcct gttcaactac aagcggctgc cagccactga cagcaacaag
     1141 cagaacctgc ggcagtactt tgaagaggct tttgagttca ttgaggaagc tcaccagtgt
     1201 gggaaggggc ttctcatcca ctgccaggct ggggtgtccc gctccgccac catcgtcatc
     1261 gcttacttga tgaagcacac tcggatgacc atgactgatg cttataaatt tgtcaaaggc
     1321 aaacgaccaa ttatctcccc aaaccttaac ttcatggggc agttgctaga gttcgaggaa
     1381 gacctaaaca acggtgtgac accgagaatc cttacaccaa agctgatggg cgtggagacg
     1441 gttgtgtgcc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc
     1501 ggtctcgatt ctacgtag
//
