LOCUS       sequence-171309-        1155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a donor vector containing N-term 3xFLAG to clone into 
            pSLIK-Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS
FEATURES             Location/Qualifiers
     source          1..1155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1132..1152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga ctagaggatc catgctgggg ctcccatcaa ccttgttcac accgcgcagt
      121 atggagagca tcgagattga ccagaagctg caggagatca tgaagcagac agggtacctg
      181 actatcgggg gccagcgtta tcaggcagaa atcaatgact tggagaactt gggtgagatg
      241 ggcagtggta cctgtggtca ggtgtggaag atgcggttcc ggaagacagg ccacatcatt
      301 gctgttaagc aaatgcggcg ctctgggaac aaggaagaga ataagcgcat tttgatggac
      361 ctggatgtag tactcaagag ccatgactgc ccttacatcg ttcagtgctt tggcaccttc
      421 atcaccaaca cagacgtctt tattgccatg gagctcatgg gcacatgtgc agagaagctg
      481 aagaaacgaa tgcagggccc cattccagag cgaatcctgg gcaagatgac tgtggcgatt
      541 gtgaaagcac tgtactatct gaaggagaag catggcgtca tccatcgcga tgtcaaaccc
      601 tccaacatcc tgctagatga gcggggccag atcaagctct gtgactttgg catcagtggc
      661 cgccttgttg acgaaaaagc caaagaacgg agtgctggct gtgctgccta tatggctccc
      721 gagcgcatcg accctccaga tcccaccaag cctgactatg acatccgagc tgatgtgtgg
      781 agcctgggca tctcactggt ggagctggca acaggacagt tcccctataa gaactgcaag
      841 acggactttg aggtcctcac caaagtccta caggaagagc ccccactcct gcctggtcac
      901 atgggcttct caggggactt ccagtcattt gtcaaagact gccttactaa agatcacagg
      961 aagagaccaa agtataataa gctacttgaa cacagcttca tcaagcacta tgagatactc
     1021 gaggtggatg tcgcgtcctg gtttaaggat gtcatggcga agaccgagtc cccaaggact
     1081 agtggagtcc tgagtcagca ccatctgccc ttcttcaggg gtggttctgg tccgaagaaa
     1141 aaacgtaaag tgtag
//
