LOCUS       sequence-17131-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Kumar S, Kao WH, Howley PM
  TITLE     Physical interaction between specific E2 and Hect E3 enzymes 
            determines functional cooperativity.
  JOURNAL   J Biol Chem 1997 May 23;272(21):13548-54.
  PUBMED    9153201
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "1997-05-23"; volume: "272(21)"; pages: "13548-54"
COMMENT     Sequence Label: p3991 pET ubcH9
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..65
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             561..587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             633..650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
       61 tgggtcgcgg atctttgaac atgtcgggga tcgccctcag cagactcgcc caggagagga
      121 aagcatggag gaaagaccac ccatttggtt tcgtggctgt cccaacaaaa aatcccgatg
      181 gcacgatgaa cctcatgaac tgggagtgcg ccattccagg aaagaaaggg actccgtggg
      241 aaggaggctt gtttaaacta cggatgcttt tcaaagatga ttatccatct tcgccaccaa
      301 aatgtaaatt cgaaccacca ttatttcacc cgaatgtgta cccttcgggg acagtgtgcc
      361 tgtccatctt agaggaggac aaggactgga ggccagccat cacaatcaaa cagatcctat
      421 taggaataca ggaacttcta aatgaaccaa atatccaaga cccagctcaa gcagaggcct
      481 acacgattta ctgccaaaac agagtggagt acgagaaaag ggtccgagca caagccaaga
      541 agtttgcgcc ctcaccgcgg tacccttatg atgtgccaga ttatgcctga tctggtacca
      601 cgcgtatcga taagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      661 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
//
