LOCUS       sequence-171310-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of constitutively active mutant MKK4 with a 
            C-terminal NLS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
  TITLE     Profiling Subcellular Protein Phosphatase Responses to 
            Coxsackievirus B3 Infection of Cardiomyocytes.
  JOURNAL   Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 
            10.1074/mcp.O116.063487. Epub 2017 Feb 7.
  PUBMED    28174228
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2017-04"; volume: "16(4 suppl 1):S244-S262. 
            doi"; pages: " 10.1074/mcp.O116.063487. Epub 2017 Feb 7"
COMMENT     Sequence Label: pSLIK 3XFLAG-MKK4-EE-NLS hygro
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1183..1203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga ctagtggatc catgcagggt aaacgcaaag cactgaagtt gaattttgca
      121 aatccacctt tcaaatctac agcaaggttt actctgaatc ccaatcctac aggagttcaa
      181 aacccacaca tagagagact gagaacacac agcattgagt catcaggaaa actgaagatc
      241 tcccctgaac aacactggga tttcactgca gaggacttga aagaccttgg agaaattgga
      301 cgaggagctt atggttctgt caacaaaatg gtccacaaac caagtgggca aataatggca
      361 gttaaaagaa ttcggtcaac agtggatgaa aaagaacaaa aacaacttct tatggatttg
      421 gatgtagtaa tgcggagtag tgattgccca tacattgttc agttttatgg tgcactcttc
      481 agagagggtg actgttggat ctgtatggaa ctcatgtcta cctcgtttga taagttttac
      541 aaatatgtat atagtgtatt agatgatgtt attccagaag aaattttagg caaaatcact
      601 ttagcaactg tgaaagcact aaaccactta aaagaaaact tgaaaattat tcacagagat
      661 atcaaacctt ccaatattct tctggacaga agtggaaata tcaagctctg tgacttcggc
      721 atcagtggac agcttgtaga cgagattgcc aaggaaagag atgctggctg taggccatac
      781 atggcacctg aaagaataga cccaagcgca tcacgacaag gatatgatgt ccgctctgat
      841 gtctggagtt tggggatcac attgtatgag ttggccacag gccgatttcc ttatccaaag
      901 tggaatagtg tatttgatca actaacacaa gtcgtgaaag gagatcctcc gcagctgagt
      961 aattctgagg aaagggaatt ctccccgagt ttcatcaact ttgtcaactt gtgccttacg
     1021 aaggatgaat ccaaaaggcc aaagtataaa gagcttctga aacatccctt tattttgatg
     1081 tatgaagaac gtgccgttga ggtcgcatgc tatgtttgta aaatcctgga tcaaatgcca
     1141 gctactccca gctctcccat gtatgtcgat ggtggttctg gtccgaagaa aaaacgtaaa
     1201 gtgtag
//
