LOCUS       sequence-171317-        9815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus to stably express fluorescent human protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Wang S, Tukachinsky H, Romano FB, Rapoport TA
  TITLE     Cooperation of the ER-shaping proteins atlastin, lunapark, and 
            reticulons to generate a tubular membrane network.
  JOURNAL   Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.
  PUBMED    27619977
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-13"; volume: "5. pii: e18605. doi"; pages: " 
            10.7554/eLife.18605"
COMMENT     Sequence Label: pHAGE2 Lnp-mCherry
FEATURES             Location/Qualifiers
     source          1..9815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1369..2076
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1514..1532)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1782..1801
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2091..2110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2092..2110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2115..2249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2193..2212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2341..2914,2915..2917,2918..2926)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2507..2524)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2734..2753
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2927..3322
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3332..3385
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3389..3409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3435..4023
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3488..3508)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3906..3917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4171..4351
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4398..4416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4484..4588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4589..4657,4658..5449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4807..4826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5620..6208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6362..6379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6619..6640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6655..6660,6661..6678,6679..6685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6693..6709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6698..6720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6717..6733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6717..6733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             6994..7627
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7674..7799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8296..8529
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8714..8758
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9054..9171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9223..9526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9527..9730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9681..9701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9727..9751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgggtggat tattttctcg atggaggaca aaaccttcaa ctgtagaagt tctagaaagt
       61 atagataagg aaattcaagc attggaagaa tttagggaaa aaaatcagag attacaaaaa
      121 ttatgggttg gaagattaat tctgtattcc tcagttctct atctgtttac atgcttaatt
      181 gtatatttgt ggtatcttcc tgatgaattt acagcaagac ttgccatgac actcccattt
      241 tttgcttttc cattgatcat ctggagcata agaacagtaa ttattttctt cttttccaag
      301 agaacagaaa gaaataatga agcattggat gatttaaaat cccagaggaa aaaaatactt
      361 gaagaagtca tggaaaaaga aacttacaag acggctaaat taattcttga aaggtttgat
      421 ccggactcaa agaaagcaaa ggagtgtgag ccgccatctg ctggagcagc tgtaactgca
      481 agacctggac aagagattcg tcagcgaact gcagctcaaa gaaacctttc tccaacacca
      541 gcaagcccta accagggccc tcctccacaa gttccagtat ctcctggacc accaaaggac
      601 agttctgccc ctggtggacc cccagaaagg actgttactc cagccctatc atcaaatgtg
      661 ttaccaagac atcttggatc ccctgctact tcagtgcctg gaatgggtct tcatcctcca
      721 ggtccacctt tagcaagacc tattctcccc cgagaacgag gtgctttgga tagaattgtt
      781 gaatatttgg ttggtgatgg tccacaaaac aggtatgcac ttatatgtca gcagtgtttt
      841 tctcataatg gcatggcttt gaaggaagaa tttgaataca ttgcttttcg atgtgcctac
      901 tgttttttct tgaaccctgc aagaaaaacc agacctcagg ctccaagact tcctgagttt
      961 agttttgaga agaggcaggt ggtggaaggt tcaagttcag ttggtccctt gccatcagga
     1021 agtgtgcttt catcagacaa ccagtttaat gaagaatctt tagaacacga tgttcttgat
     1081 gataatacag agcagacaga tgacaaaata ccagctacag aacagacaaa ccaagtgatt
     1141 gaaaaagcat ctgactcaga ggaaccagag gagaaacaag agactgagaa tgaggaagcc
     1201 tcagtgattg aaaccaactc cacagttcct ggagctgatt ctattcctga tcctgaacta
     1261 agtggagaat ctttgacggc agaggctagt ggtggaggct ctgggggagg tacaggaggt
     1321 ggctcaggtg ggggcactgg tggaggcagt ggaggtggca gatccaccat ggtgagcaag
     1381 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1441 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1501 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1561 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1621 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1681 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1741 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1801 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1861 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1921 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1981 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2041 cgccactcca ccggcggcat ggacgagctg tacaagtcta gattctgcag ccctatagtg
     2101 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2161 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2221 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2281 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgctaagtaa gtaagatccg
     2341 cccctctccc tccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     2401 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     2461 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     2521 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     2581 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     2641 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     2701 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     2761 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     2821 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     2881 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg ccaagccttt
     2941 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     3001 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     3061 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     3121 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     3181 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     3241 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     3301 ctctggttat gtgtgggagg gcggtagtgg cgagggcaga ggaagtcttc taacatgcgg
     3361 tgacgtggag gagaatcccg gccctgctcc aaagaagaag cgtaaggtaa atcgatcgct
     3421 agtcgagaga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3481 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3541 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3601 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3661 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3721 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3781 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3841 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3901 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3961 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4021 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4081 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tcacctgcag
     4141 gacaggcgcg ccctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4201 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4261 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4321 cccttttagt cagtgtggaa aatctctagc acccgggcga ttaaggaaag ggctagatca
     4381 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4441 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4801 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5761 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6301 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6361 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6421 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     6481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6541 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     6601 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6661 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6721 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
     6781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     6841 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     6901 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     6961 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     7021 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     7081 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     7141 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     7201 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     7261 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     7321 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     7381 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     7441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7621 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7681 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactgcaga
     7741 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7801 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7861 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7921 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7981 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8041 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8101 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     8161 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8221 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8281 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8341 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8401 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8461 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8521 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8581 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8641 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8701 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8761 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8821 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8881 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     8941 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     9001 cggatctcga cggtatcgcc gaattctcaa caataggcag tattcatcca caattttaaa
     9061 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     9121 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     9181 tactcggaca gcagagatcc agtttggact agtggagttc cgcgttacat aacttacgca
     9241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgaccaa
     9301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     9361 caaaactgcc cacttggcag tacatcaagt gtatcatttg ccaacaacgc cccctattga
     9421 cgtcaatgac gcaaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     9481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     9541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     9601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     9661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     9721 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     9781 cctccattca agacaccggc ggccttaatt aaacc
//
