LOCUS       sequence-17132-e        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Scheffner M, Huibregtse JM, Howley PM
  TITLE     Identification of a human ubiquitin-conjugating enzyme that mediates
            the E6-AP-dependent ubiquitination of p53.
  JOURNAL   Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801.
  PUBMED    8090726
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1994-09-13"; volume: "91(19)"; pages: 
            "8797-801"
COMMENT     Sequence Label: p3992 pET ubcH5
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(538..556)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      552..599
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gnttctcnga atattttgtt tactttaaga aggagatata catatggcgc tgaagaggat
       61 tcagaaagaa ttgagtgatc tacagcgcga tccacctgct cactgttcag ctggacctgt
      121 gggagatgac ttgttccact ggcaagccac tattatgggg cctcctgata gcgcatatca
      181 aggtggagtc ttctttctca ctgtacattt tccgacagat tatcctttta aaccaccaaa
      241 gattgctttc acaacaaaaa tttaccatcc aaacataaac agtaatggaa gtatttgtct
      301 cgatattctg aggtcacaat ggtcaccagc tctgactgta tcaaaagttt tattgtccat
      361 atgttctcta ctttgtgatc ctaatccaga tgacccctta gtaccagata ttgcacaaat
      421 ctataaatca gacaaagaaa aatacaacag acatgcaaga gaatggactc agaaatatgc
      481 aatgtaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      541 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      601 tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag
      661 tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac
      721 agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg
      781 ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc
      841 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
      901 gcattgntag attcatacac ggtgcctgac tgcgttagca tttaactggg anaactacng
      961 cattaagctt atcgatgata nctgtcaaac atgaaaattc ttgaaaaaaa aggccncggn
     1021 ancccctatt tttaaggtta ggccaggaaa aaagggtttc taaaactcng gggncctttn
     1081 cgggaaatgn gncggaaccc nattgttttt ttcnaaaaca ttcaattgtt cnccngnaaa
     1141 aaaanccnga naangctcat aantgaaagg anaaaggatt tcccttncgg gncctttncc
     1201 tttgggcatt gcctcggttt gcccccaanc cnggnaaaaa aaggaaannt ngggccaagg
     1261 ttcccctnnt cccccgnaac ctganttccc caaattccan aacttanttt tgggggnttc
     1321 ggggcgg
//
