LOCUS       sequence-17133-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Scheffner M, Huibregtse JM, Howley PM
  TITLE     Identification of a human ubiquitin-conjugating enzyme that mediates
            the E6-AP-dependent ubiquitination of p53.
  JOURNAL   Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801.
  PUBMED    8090726
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1994-09-13"; volume: "91(19)"; pages: 
            "8797-801"
COMMENT     Sequence Label: p3521 pET ubcH6
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             581..598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(651..669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggatgacgat tcgagggcca gcaccagctc ctcctcatct tcgtcttcca accagcaaac
       61 cgagaaagaa acaaacaccc ccaagaagaa ggagagtaaa gtcagcatga gcaaaaactc
      121 caaactcctc tccaccagcg ccaagagaat tcagaaggag ctggcggaca tcactttaga
      181 ccctccacct aattgcagtg ctggtcccaa aggcgataac atctatgaat ggagatcaac
      241 cattctagga cctccaggat ccgtgtatga gggtggtgta ttctttctcg atatcacttt
      301 tacaccagaa tatcccttca agcctccaaa ggttacattt cggacaagaa tctatcattg
      361 taatattaac agtcaaggtg ttatttgctt ggacatattg aaagataatt ggagtccagc
      421 actaaccatt tctaaagtcc tcctttctat ctgctcactt cttacagact gtaatcctgc
      481 cgaccccttg gtgggaagta ttgccactca gtatatgacc aacagagcag aacatgacag
      541 aatggccaga cagtggacca agagatacgc tacactcgag caccaccacc accaccactg
      601 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      721 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      781 ggtgtggtgg g
//
