LOCUS       sequence-171330-       20517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned 
            into Pst/Bam.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20517)
  AUTHORS   Jones KM
  TITLE     Increased production of the exopolysaccharide succinoglycan enhances
            Sinorhizobium meliloti 1021 symbiosis with the host plant Medicago 
            truncatula.
  JOURNAL   J Bacteriol. 2012 Aug;194(16):4322-31. doi: 10.1128/JB.00751-12. 
            Epub 2012 Jun 8.
  PUBMED    22685282
REFERENCE   2  (bases 1 to 20517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.00751-12"; journalName: "J Bacteriol"; date: "2012-08"; 
            volume: "194"; issue: "16"; pages: "4322-31"
COMMENT     Sequence Label: pstb-LAFR5-exoY
FEATURES             Location/Qualifiers
     source          1..20517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2104..3252
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4525..5724)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             5830..6480
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    6788..7186
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     rep_origin      7364..8075
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     8431..8453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8445..8462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8446..8462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9634..9650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9634..9650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9647..9669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9658..9674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9682..9688,9689..9706,9707..9712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9727..9748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     oriT            18698..18807
                     /note="incP origin of transfer"
     CDS             18840..19211
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ggtgatgtac tggccgttct cgtaaccaag aacttcgaga atttcttgca ctcgacggcc
       61 gctaggggtc ctggcgatat ggacgaccac atgaaccgcc tcgccaatca gcggctcaat
      121 gggtttcggt gaatccgggt gcatgctgat aagcatggcg agccggctca ggcccgcttt
      181 ggggttgttt gcgtgcaggg tggcggcacc tccttcatgc ccggtgttcc aggccatcaa
      241 cagatcaagg gcttcggggc cacgtacctc accgaccagg atgcggtcgg ggcgcatacg
      301 cagcgttgtc ttgagcagca gcgtcatcga gacgtcgatg ctggtgtggt attggacggc
      361 gttctctgcg gcgcactgga tttcgccggt gtcctcgatg atgacgacgc gctcagacgg
      421 gttgaaggcg accatttcat tgatgatcgc gttgacgagc gtggtcttgc ccgagccagt
      481 accgccaatg acgaggatgt ttcgatgcgc cgcgacggcg cttttaatga cctcgtattg
      541 ctcgcgggtc atgatgcccg cctcgacgta ctgttccagc gtgaagatgg cgaccgcgcg
      601 cttgcggatc gcaaaggttg gcgcggccac gaccggcggc aattggccgg caaagcggct
      661 gccatccaag gggaactcgc cttccaggat gggcgaatgc cgcgtgacct ctttgccgtg
      721 gaatccggcc accgtttcta taatcgcctg cgactggctg ggccgcatgt cgcagatgta
      781 ccgcatcggc tcgccaaggc gttcgtgcca caccttgccg tccgcgttga gcatgacttc
      841 aacggttttc gggtcgttga gcgcggccaa caggtccgca cccatgtcgc gttccagctt
      901 gcgcttggcc cgctccttga tggtctgaaa ctcttctttc ccgctcactt ttcagtaacc
      961 cctacgcaac aaatcattag caacattatc gcacaggcaa acgatatacc gaaagtgttt
     1021 agcctgcaag acaaacagct aatcgaaccg cacgacgtag gggttctgat agaaaacaac
     1081 tgtcaaagcg cacccgcccg atgccattcg cggcacggct tccgttgagg atgtcgatat
     1141 gatgcgcgag ccgacggccc gcagagaagg ggccgtttta gcggctaaag aaggaagtgc
     1201 aagccctaac ccttggcgtc agagccttcc acgcagcttt tttcgggtgt cgtcgcccca
     1261 tttctttacg ataaacgcct tatgtgacgg caaaaccaca ctgatgcgtt cgtatccggg
     1321 cggcacgctg ctcttgaaag gatgacccgc aatctccgcg agtgcctcgc ggtcaaggtc
     1381 ggtggactcc aggagaagag gtaggggagt ttccagggcg tcggcaatgg cctccatcac
     1441 cttcaacgag gggttggcct taccgttggt taagtctgat aaaaacgaaa ttgaaacccc
     1501 tgccctctcc gacagctcat gtttcgtcat gccccgctca tcgagcagac gaaggatgtt
     1561 ggtgaaaaat atctggttgt acacagcgga agccgcccct cgcacctttg gtcgcggccc
     1621 gcaaaatttt agccgctaaa gttcttgaca gcggaaccaa tgtttagcta aactagagtc
     1681 tcctttctca aggagacttt cgatatgagc cataatcagt tccagtttat cggtaatctt
     1741 acccgtgaca ccgaggtacg tcatggcaat tctaacaagc cgcaagcaat tttcgatata
     1801 gcggttaatg aagagtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
     1861 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
     1921 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     1981 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     2041 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     2101 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     2161 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     2221 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     2281 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     2341 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     2401 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     2461 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     2521 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     2581 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     2641 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     2701 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     2761 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     2821 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     2881 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
     2941 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
     3001 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
     3061 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
     3121 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
     3181 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
     3241 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcttta
     3301 ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca gtatcgctcg
     3361 ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg acaattgtga
     3421 ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc gagatccgat
     3481 tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac gaggagaaaa
     3541 agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc gcctacatcg
     3601 acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag gacgctcaca
     3661 aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg gtcgccggta
     3721 tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga cagattccaa
     3781 cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg ctattctgga
     3841 gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg gtaggcgctg
     3901 tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg atacgattga
     3961 tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg ttgacaccaa
     4021 acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt tccatggcgt
     4081 tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc tttaccgcct
     4141 ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt gatccgccaa
     4201 tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg atcggagcgg
     4261 gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca gttgtttcct
     4321 tactgggctt tctcagcccg gggaccgccg tgttgctagg atggttgttc ttggatcaga
     4381 cgctgagtgc gcttcaaatc atcggcgtcc tgctcgtgat cgggagtatc tggctgggcc
     4441 aacgttccaa ccgcactcct agggcgcgta tagcttgccg gaagtcgcct tgacccgcat
     4501 ggcataggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc cgctccaaag
     4561 cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca
     4621 cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat
     4681 cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc
     4741 cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag
     4801 gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt
     4861 gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg
     4921 gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc
     4981 gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg
     5041 cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat
     5101 gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta acggccggcg
     5161 ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa
     5221 ggctgccgcg gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac
     5281 aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg
     5341 ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt
     5401 gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgctg tcgccatgat
     5461 ggcgtagtcg acagtggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg
     5521 atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag
     5581 cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg
     5641 cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac
     5701 gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg
     5761 aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa
     5821 gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg
     5881 aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag
     5941 cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa
     6001 gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca
     6061 tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg
     6121 cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt
     6181 cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc
     6241 agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag
     6301 cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc
     6361 ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga
     6421 ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga
     6481 tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag acaacatccc
     6541 ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct
     6601 gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctcct
     6661 gctgcggcgt ccgctcgtgg gccgtggcgc gggtccgcgc gccggcctcg tgcgcctggc
     6721 gctcgcgggc gaggtccagg gcggccgtct tcacgttctg ccttgcgcag atgagataga
     6781 tccggacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa
     6841 aaagctcgct ttcagcacct gtcgtttcct ttcttttcag agggtatttt aaataaaaac
     6901 attaagttat gacgaagaag aacggaaacg ccttaaaccg gaaaattttc ataaatagcg
     6961 aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg
     7021 ataatgatta tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa
     7081 tcaattatga cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt
     7141 cagacaatac aaatcagcga cactgaatac ggggcaacct catgtccgga tctagcgtgg
     7201 actcaaggct ctcgcgaatg gctcgcgttg gaaactttca ttgacacttg aggggcaccg
     7261 cagggaaatt ctcgtccttg cgagaaccgg ctatgtcgtg ctgcgcatcg agcctgcgcc
     7321 cttggcttgt ctcgcccctc tccgcgtcgc tacggggctt ccagcgcctt tccgacgctc
     7381 accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca
     7441 gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg
     7501 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
     7561 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
     7621 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
     7681 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
     7741 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
     7801 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
     7861 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
     7921 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
     7981 ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc
     8041 ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gcagtccttg ccattgccgg
     8101 gatcggggca gtaacgggat gggcgatcag cccgagcgcg acgcccggaa gcattgacgt
     8161 gccgcaggtg ctggcatcga cattcagcga ccaggtgccg ggcagtgagg gcggcggcct
     8221 gggtggcggc ctgcccttca cttcggccgt cggggcattc acggacttca tggcggggcc
     8281 ggcaattttt accttgggca ttcttggcat agtggtcgcg ggtgccgtgc tcgtgttcgg
     8341 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8401 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     8461 gtgccaagct tggcacgcgt ctgaatgtcc attgttccct cccgaaatcg tgttccccga
     8521 gacagtcctc tctgtcccgg cgggagtatt tctcaaaact cgggtagacg caattatttt
     8581 cagtaaattt ttacctcgtt tttatgagcg gcgacggcgc tttcggccgt ctagcctgca
     8641 caagccatct tgctagcatc gcatgcgtct cctatattgg gggaaaggag tattgaggca
     8701 tgggtgcccc acagctttcg gtccgaagtg caaaagcgcg tgatctggcg cgccgacttg
     8761 cacggcgaga gaaccgctcc atcgctgaca tcgtcgagcg tgctcttgag ttatatgaaa
     8821 ccagagaagc tggccgagag cctgcgagtg ctttctatgc gcgcttgttc gagagttgcg
     8881 gcacggacat cgacctgcag taacgtaacg taaatggagt cacctctatg aagtccgcga
     8941 cgcgctcggc cagttcgccg ttttttatcc ccgaagaaac cggggcagtc cgaccgatcg
     9001 gaggtatggc aaaacgcagt ttcgacgttc ttgccgcctc cgtggccctc cttctcttca
     9061 gcccgctctt tttgctcatc atggccctcg tcaagttctc ggacggcggc agtgtctttt
     9121 atgggcatcg ccgaatcggt cacaatggcc aatccttcaa gtgcctgaaa ttccgtacga
     9181 tgatggaaaa gggcgacgag gtcctcgaag agtttttcag gatcaacccc gacgcctatg
     9241 aagaatggcg tgcgacccgc aagcttcaga acgatccgcg cgtgaccgtt gtcggagcag
     9301 tcctgcgtaa gctcagcctc gacgagttgc cgcagctcct caacatcatt cgcggtgaaa
     9361 tgagcgtcgt cggcccccgt ccggtggtcg aagacgaact cgaactctat gattcggcag
     9421 ccgtctttta tctgcgatcg cgtcccggcc tgacggggct ctggcagatc agcggccgca
     9481 acgacgtctc ctacgccacg cgcgtcgctt tcgacacgca atatgtccag aactggtcgc
     9541 ttttcgccga ccttgtcatt gttttcaaga cgatccctgc cgtttgcctc tcccgcggca
     9601 gctactgagg atccccggga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9841 gcgtatgggt tcgtgcgatc cgtctgcatg atggtgatag ccgtcagcat gattttcgtg
     9901 tcgtcgaact tggtgaaggg cattctcggc ggcgatcacg acgccggccc tgcggagcct
     9961 tcgccgcgtg cgcgattcat ggcggccgtg gaggccaagg atttcgcgcg agtgcaagag
    10021 ctgatcgagg cgcgtggagc caagtcggcg gctgattatg tccttgcgca gctcgccgtg
    10081 gccgaaggtc tggaccgcaa gcctggtgcg cgcgtcgtgg tcgggaaagc ggcgggcagc
    10141 atggcaatgc cgcctgcggc gctgggtttt acgccaaggg gagaagcggc atacgccatc
    10201 gagcggtcag cctatggtga gccgaggtcc agcattgcga agcagtacca gcaggaatgg
    10261 aaccggaagg cggcgacctg gtgggcgatg gccggtgtgg ccggcatcat cggcgcgatc
    10321 ctggcggcgg cggcaaccgg ctttgttggg ctggcagtgt cgatccgcaa ccgagtgaag
    10381 cgcgtgcgcg acctgttggt gatggagccg ggtgcagagc cataagcggc aagagacgaa
    10441 agcccggttt ccgggctttt gttttgttac gccaaggacg agttttagcg gctaaaggtg
    10501 ttgacgtgcg agaaatgttt agctaaactt ctctcatgtg ctggcggctg tcaccgctat
    10561 gttcaaccaa ggcgcggagc aaattatggg tgttatccat gaagaaacgg cttaccgaaa
    10621 gccagttcca ggaggcgatc caggggctgg aagtggggca gcagaccatc gagatagcgc
    10681 ggggcgtctt agtcgatggg aagccacagg cgacgttcgc aacgtcgctg ggactgacca
    10741 ggggcgcagt gtcgcaagcg gtgcatcgcg tgtgggccgc gttcgaggac aagaacttgc
    10801 ccgaggggta cgcgcgggta acggcggttc tgccggaaca tcaggcgtac atcgtccgga
    10861 agtgggaagc ggacgccaag aaaaaacagg aaaccaaacg atgaaaactt tggtcacggc
    10921 caaccagaaa ggcggcgtcg gcaagacttc gacccttgtg catcttgcct tcgacttttt
    10981 cgagcgcggc ttgcgggttg ccgtgatcga cctggacccc cagggcaatg cgtcctacac
    11041 gctcaaggac tttgctaccg gcctgcatgc aagcaagctg ttcggcgctg tccctgccgg
    11101 cggctggacc gaaaccgcac ccgcagccgg cgacggccag gccgcgcgcc tcgccctcat
    11161 cgagtccaac ccggtactgg cgaacgccga acggctgtcg ctggacgacg cccgcgagct
    11221 gttcggggcg aacatcaagg ccctggcgaa ccaaggcttc gacgtgtgcc tgatcgacac
    11281 ggccccgacc cttggcgtcg gcctggcggc cgccctcttc gcggccgact atgtgctgtc
    11341 ccccatcgag cttgaggcgt acagcatcca gggcatcaag aagatggtca cgaccattgc
    11401 gaacgtgcgc cagaagaacg ccaagctgca attccttggc atggtgccca gcaaggtcga
    11461 tgcgcggaat ccgcgccacg cgcgccacca agccgagctg ctggccgcgt accccaagat
    11521 gatgattccg gccaccgttg gcctgcgcag cagcatcgcc gatgccctcg catccggtgt
    11581 gccggtctgg aagatcaaga aaacggccgc gcgcaaggca tcgaaagagg ttcgcgccct
    11641 ggctgattac gtgttcacga agatggagat ttcccaatga ctgcggctca agccaagacc
    11701 accaagaaaa acaccgctgc ggccgctcag gaagccgcag gcgcggcgca gccgtccggc
    11761 ctggggttgg atagcatcgg cgacctgtcg agcctcctgg acgctcctgc ggcgtctcag
    11821 ggcggttccg gccctatcga gctggacctg gacctgatcg acgaagatcc gcatcagccg
    11881 cggacggccg acaaccccgg cttttccccg gagagcatcg cggaaatcgg tgccacgatc
    11941 aaagagcgcg gggtgaagtc acccatttcg gtgcgcgaga accaggagca gccgggccgc
    12001 tatatcatca atcacggcgc ccgccgctac cgtggctcga agtgggccgg caagaagtcc
    12061 atcccggcgt tcatcgacaa cgactacaac gaagccgacc aggttatcga gaacctgcaa
    12121 cgcaacgagc tgaccccgcg cgaaattgcc gacttcattg gccgcgagct ggcgaagggc
    12181 aagaagaaag gcgatatcgc caaggaaatc ggcaagtcgc cggcgttcat cacccagcac
    12241 gtcacgctgc tggacctgcc ggagaagatc gccgatgcgt tcaacaccgg ccgcgtgcgc
    12301 gacgtgaccg tggtgaacga gctggtgacg gccttcaaga agcgcccgga ggaagtcgag
    12361 gcgtggcttg acgacgacac ccaggaaatc acgcgcggca cggtcaagct gctgcgcgag
    12421 ttcctggacg agaagggccg cgatcccaac accgtcgatg ccttcaacgg ccagactgat
    12481 gccgagcgtg acgcggaggc cggcgacggc caggacggcg aggacggcga ccaggacggt
    12541 aaggacgcca aggaaaaggg cgcgaaggag ccggacccgg acaagctgaa aaaggccatc
    12601 gtccaggtcg agcacgacga gcgccctgcc cgccttatcc tcaaccgtcg gccgccggcg
    12661 gaaggctatg cctggttgaa gtacgaggac gacggccagg agttcgaggc gaaccttgcc
    12721 gacgtgaaac tggtcgcgct catcgagggc tgatccccaa agacagcggc gcgggccacc
    12781 cgcgccgcac agacaacggt tccgctacaa ggaggaccga agaatgaatc cgatgctgtt
    12841 ctacatcgcg ggaggcgtag gcgcggcgtt gctgctggtt tccgcgatca tgctgttcaa
    12901 gctgcgcgag ccgaagaagg aacaccgacc gcagcgcaag gcggcggccc cgacgccgca
    12961 gccggtcgat aacgagctgc tgcgcacctt tcgccggccg tcgcaggcca gccaacccga
    13021 gccatccacg acgccgccgg cgcggcgcac tgccccgccg agcagcgaac aatccagagg
    13081 gaatgacggc gatttcgtca cgtcggccct ggtggccggg gcgaccaaca gcaccatgct
    13141 gggctacctg gccggcggct cgctgaccgg ggccatgctg ggcgatgcgc tgcggccgga
    13201 tacgccgtct gcggcgttcg cggacccctc gccgtgcttc ggcagcagcg acacggacag
    13261 cggctggacc gataccggca gctcttgcga tttcagttcg tccgacaccg atacgaacaa
    13321 ctggtgaggg cggaccatga ctgacgaaca aaagaaacgg ctcctgatgt tgcaagctat
    13381 ccggcgcagc atccaggcaa tgcgcagggg tgtttcgggg ccggatagtt tcaagggctg
    13441 gttgaccggc atcggcatca tcgcggcggg gttcctggtt ggcgagctgc tgccatcccc
    13501 tgcccccggc gtcggcctgg ttgtgccagg ggcctgcatt gccggcttcg tcgtcggctg
    13561 ctggctcaac aggaaaggcc gctcatggcc gacagccatt tacgaccaat tggcccgtta
    13621 ccagccgctc aacgagcggg cgtaccgcga cctgcaagag acggtgaagg aacgcggcct
    13681 cgactgcgat gcggtcatgg agtggaccta catcgagcag gccgagttca tgcccctggt
    13741 gcccaccagg gaggacctag cgcgcgacaa gttcctgacc gcgacgcggg ccgatagggc
    13801 cgaggacgat gcgcgtgcgc gcttcgtcgt gctccatccc aagaagcgcg gccccgacgc
    13861 atgaagaagc ccgacgcccg ctaatcccct tcccgctgac cctttgcccc cgccgaccgt
    13921 gcggggcttt ttttgcctgc ccttcagccg ctaaaaaacc tgccgttaag tcgagaactg
    13981 ttcagctaaa ctcttgcgct gataaggtag gtaagagtat tattattctt accaattacc
    14041 ggagccaaca tggaactgaa caaagagacg cgcgaccgca tttttgcggc tgcggacgag
    14101 ctgttcgagc aaggcgaccg ggagaacttc ccgaccgtgg atgcagtgcg taaggccgcc
    14161 cgcgtcaaca tgaacgacgc cagcgccggc atgcgcgaat ggcgacgcca gcgcacggcg
    14221 caggccgccc cgctggcagt ccaggtgccg gacgcggtgc agcaggccgg caaccaggcc
    14281 gtggccgccc tctggcaagc cgcccaggcc cttgccaatg aatcgttgca ggcagcgcaa
    14341 gccggctggg atcgtgagcg caacgagttg gaagccgtgc gcggggagct ggccgacgcc
    14401 ttcgaggcgc aggcccgcga gctggaagaa gcccaggcgc gcgttacatt gctagagcag
    14461 caggccgccg aggcggccga gctggccgca cgccagcggc aagccctggc cgaagcacgc
    14521 accgccctgg caggagccga gcagcgggcc gcgctggcga cgcagcgggc cgacgaggtg
    14581 gagcgtcgag cgcaggagct gcgcgccgag ctggactacg ctcaccagga cgcccgcgca
    14641 ttcaaagaag agagccgcaa gaccctggac gcggccaacc aggaaatgca ggcgcttcgc
    14701 ggcgagcgcg acgccaccag gcgcgaagcg gagagtttgc gcacggagtt ggcagcggtg
    14761 aaagcgaagg cgcaggccga cgccgaggcc caccaggagc aaaggaagtt ggcggcgcaa
    14821 gaggcggcca ggcaggccga gcgctttacg acggtgcaag ccgagcgtga cgaagcgcgg
    14881 caagaggcaa aggcagccca agaagaggcc gctatgttga ggggtcggct agacgcggaa
    14941 acgagtggga ggaaacacaa agcgggagga aagtaactga taaatcgaac gcagttcact
    15001 aaaatagatg gaacaacctt gacgcgcccg gctctaggga aagccggcct cgaactgccg
    15061 aggtcggctt ttttttagcc ggcaagcgac caggacacgt ttaacaccag aggcaaccgt
    15121 caggagtcac ccaatgaaac accgcctcct ggtcatgaat gggcaaagaa ttgtccagac
    15181 cgagaaccaa ggcgcatgga cgaaccagaa ggtagataaa gccggggccc tgaaacctgg
    15241 catttacaac atctatatgg cccaaaaggc cgataagtcg cagcgccacg acggcagcat
    15301 cgtccacgca gatagcggca gcatttacca acaggtgggg aagaactttg taatgcacgc
    15361 ccggtcagat ttcgataaag tacctgaaat cgggagtgcg aaaagcatca cctatgacgc
    15421 aagtggcaag gcacaggtaa gtgccgaaag cgtcaagttg agtcgcgggc gcactcgtta
    15481 gagttttagc ggctaaaaag ggaggcgtct tgagcagcta cagcagagcc gagcgcgcac
    15541 cgtggggaga cttcccgaag gtggttcgca acggcgacct tggttctttg accaacgagc
    15601 ctgaatacca ggctgcgaag cagggcgacg cggaagcggc gcttaatttg gtcgagcgcc
    15661 tgatttcgga cgacactgtt gcccagctca agacgctgat cggcgacgac aagccccgca
    15721 tcgttccagt cctcgccgtc gaggcggcgg ggaacaacaa gattccggcc atgatggcgg
    15781 tcgttctggc cgaccgcctt ggccttgagg tcgaaacgga cattgtgcag cgggagaagg
    15841 tcgcccgaac cggagccggt tcagatcatc gcctggcgtt caacccgact ttcgaggggg
    15901 aggtgatccc cggccagaag tacatcgttg tcgatgacac gctgacgatg ggcggcacca
    15961 ttgcctcatt acgcggctac atcgagaaca acggcggcaa ggtcatggcg gcgtccgtca
    16021 tgaccgccca tgaaggcgcg cttgacctgg cggtcaagcc gaagatgctt gcgggcatca
    16081 atgagaaaca cggtccagca atggacgcat tttggaagga aacctttggc tatggcatcg
    16141 accgactcac ccaaggagaa gccggacacc tccgcgctgc cccgtccgtt gacgcaatcc
    16201 gagatcgcat cgctgcggct cgaaatgaag cagtcaatcg agtgggggca agccgaactg
    16261 cgacgacggc gcgagcaggg caacagcagt ccccggcagt aaagcagtcc agcggcaaca
    16321 gcggcgagga tttgctacag gcggcccagg aggccgagac ggaacagcag gccctcttag
    16381 aatccgcgcc catcgagcag acctaccagc agacccttgc gctctacgtg caggcaaagc
    16441 atgaccaggt ggagcgcatc gaggaccgcc tagaacagct cgtagaccgt cagcaggcgc
    16501 gtttgcagca gctacagtcc aatgcccccg gcttgctgtc cctgccgcgt acaaaggccg
    16561 cctggcagca gcagcaggcc caacagcaag cccgcttgca gaccctgcac acgcggctgg
    16621 acatggtgcg cgaaatcaag gaaggcatgg gcattcacgc gcccaagatc gaggaactgg
    16681 cgacgcgcaa gatgcgcgcg gagcatcccg agctggccgg cgactgggat tcgatgcgtg
    16741 aagcagcccg acggcaccag gccctgatgc gcaagcagga gcaggagaag aaacaggccc
    16801 aggagcgcga gcgcgggcgc agtcagagct tgggcctgtc aaacaagccc agctaacgca
    16861 aaaacaaagc ccggcaacgc cgggcttttt catctgcgcc tctgcgattc ataacgaggc
    16921 ccacaccacc agggcggccg cgaacaacac catgccgccg gcgatcatgt tcatcatcgc
    16981 cacgcgccgc gcgtccgcga ccttggccgc gagctggcgg ccaaggccgt cgtcgatttc
    17041 cctgcggatc gcttcggccg cctgcgcggc gctgtccttc attaccttcg ccattgcgtc
    17101 cttgctggcc gccagggccg cgttcagcat ccgctccgct ttggccttgg cgtcctcgcc
    17161 ccaacgatgg gcgatccctt ccagctcttc cttgaacgcg gcaaggattt cctcttgctt
    17221 ggccgcactg tcggccatga gccgggcgtt gatggtatgc aggatcagca ccgggtcgtc
    17281 gcggccgacg gcgatgccgt gcttggccgc aatctcccgg atcagctctt caatctggtc
    17341 gctcatagca cggccgccgc gtcgagctgt tcaaacaggc cgcgccgcac gatcttgagg
    17401 cgttgccgcg tcatgatcgt gagcgattca tcggccagcg cctggtcgaa cgtcagccgc
    17461 tcttgcagca tgtcgctgaa atcgcggccg taggtttctt ccttgagggc cggaatctgg
    17521 atgatggacg acacgcgggc cttgttggcc gtgtacgcct tcatctgctc aaagctcttg
    17581 ccctcatgct cgataggccc ccaatacggg ttcagccaga ccacgaaaag cgcttcggcc
    17641 gggaactggc tggcgagctg ggcgaagccg ctcaccgtgt ccaggagagc ctggccgccg
    17701 gtgacgacgg tatggatgac cagctcatgc cccatttctt gcagcagagc cggcacctgg
    17761 ttgctgatga ggtaatgcga cagaggcacg aacgagctgg caccgttgtc gatcaccacg
    17821 tcatccttgg tcggcgcaat cagctcgacc agggtgtcga agttgcgcga gttaatttcg
    17881 tcgccggcca tgatgttcag ccggcggacg ttcagggcct tgtagccctc gaacgtcgcg
    17941 ttcaccgggt cggtgtcgat gcacaagggt gtctgcccct tgtccatctt gtactgcgca
    18001 atgatcgcgg cgatggccga cttgccgacc ccgcccttgc cctgcaaaac catgtgaatt
    18061 ttcgccatta cagtagatcc tttttgtccg gtgttgggtt gaaggtgaag ccggtcgggg
    18121 ccgcagcggg ggccggcttt tcagccttgc ccccctgctt cggccgccgt ggctccggcg
    18181 tcttgggtgc cggcgcgggt tccgcagcct tggcctgcgg tgcgggcaca tcggcgggct
    18241 tggccttgat gtgccgcctg gcgtgcgagc ggaacgtctc gtaggagaac ttgaccttcc
    18301 ccgtttcccg catgtgctcc caaatggtga cgagcgcata gccggacgct aacgccgcct
    18361 cgacatccgc cctcaccgcc aggaacgcaa ccgcagcctc atcacgccgg cgcttcttgg
    18421 ccgcgcggga ttcaacccac tcggccagct cgtcggtgta gctctttggc atcgtctctc
    18481 gcctgtcccc tcagttcagt aatttcctgc atttgcctgt ttccagtcgg tagatattcc
    18541 acaaaacagc agggaagcag cgcttttccg ctgcataacc ctgcttcggg gtcattatag
    18601 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc
    18661 gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc
    18721 cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg
    18781 ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga
    18841 tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg tactgccttc
    18901 cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct
    18961 acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc
    19021 gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg aaactctggc
    19081 tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga
    19141 agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc cgcccgaggg
    19201 cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac
    19261 gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtgaagta catcaccgac
    19321 gagcaaggca agaccgagcg ctcttcttca atcgctgccg tcagacggtc agggaacgcc
    19381 gacaggcccc gctccgcgta gttcggagca ggacgccgca cggcgtccac atggatcatc
    19441 cacccacttc ccaggcccgc gagggcctgg ttgatgcggg cggacactac ttcgcgctgc
    19501 tggtcggtgc tgcttgcgtt gtcatcgccc ttgtacagcc aggcagccat aaagctgccg
    19561 ttcttgccca cgattacgcc gtcatcgacg acagcggcgt agttgagcag atcggccagg
    19621 ccggcgtcct tggaacgatg ctttttcagt ttcagttcgg catcgaccgc gcggatgcgg
    19681 gcaaagagga tgaacaacag aagcgcgccg aggcccgcga ttgcaatcgc aattgcttgg
    19741 atcatcggta ttgcttccct tggctattgg tgttctcgcg gaacggggtc gagcgggccg
    19801 ggtaatacgg cttgtaccgg cggtgacgca ggtacacgaa ccgcatcttc ggatcggcct
    19861 tcgccatgat tcggaacgca tagagcgccc cgaaccacag gatcagaccg accacggtgg
    19921 cccgcagctc ttgggcgctg aaaatcagcg caaacgccat caggcccgag aacatcacca
    19981 gttcacgatc accacccatg aacaggtttt ctcggttgcc tgcgcgacgg atggggatcg
    20041 tgcgcagagc catgattagg cgagccgtcc agccgctacc gcacgcacgg catccgccgc
    20101 cgcgacttgc acctggtgca gcgccccgtt gccgagggcc gcgatttcgg caccacgacc
    20161 gaagaaggtg ctcatcacgt tctgcgcgcc gaccagcagc gccatcacca gaaccaggaa
    20221 gatcagggtt cggaagaagg cgttgagttc gccgccgaag atcagcacgc cgccggcgac
    20281 gacgatgccg atgatggaca gcgcgaaggc caccgggccg gttacggagt tgcgcaggtt
    20341 cgtcagccag ctctcatatg gcaagctgcc gccggtgcct tccgaggcca tcgccggatg
    20401 cgcggataac gcgagagcga gaacgaagaa cacggcaagg tagaacaaaa tgccgcgatt
    20461 catggtcaga cggaacggaa cagccgttgt cattggaaat actccttaca gggtttt
//
