LOCUS       sequence-171331-        4970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fusion of PTEN with a modified human FKBP variant harboring the F36V
            and L106P point mutations (FKBP*) leads to degradation of the fusion
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   An W, Jackson RE, Hunter P, Gogel S, van Diepen M, Liu K, Meyer MP, 
            Eickholt BJ
  TITLE     Engineering FKBP-Based Destabilizing Domains to Build Sophisticated 
            Protein Regulation Systems.
  JOURNAL   PLoS One. 2015 Dec 30;10(12):e0145783. doi: 
            10.1371/journal.pone.0145783. eCollection 2015.
  PUBMED    26717575
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-12-30"; volume: "10(12):e0145783. doi"; pages: " 
            10.1371/journal.pone.0145783. eCollection 2015"
COMMENT     Sequence Label: FKBP*-PTEN
FEATURES             Location/Qualifiers
     source          1..4970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     249..271
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     333..352
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(393..395,396..716)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLSSGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     primer_bind     complement(2027..2046)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2092..2147
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2146..2165)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2553..2570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2724..3312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2804..2823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3483..4274,4275..4343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4106..4125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4344..4448)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4479..4858
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
       61 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
      121 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
      181 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
      241 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
      301 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
      361 tcattttggc aaagaattcg aagcttgcca ccatgggagt gcaggtggaa accatctccc
      421 caggagacgg gcgcaccttc cccaagcgcg gccagacctg tgtggtgcac tacaccggga
      481 tgcttagcag cggaaagaaa gtcgattcct cccgggacag aaacaagccc tttaagttta
      541 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
      601 gtcagagagc caaactgact atatctccag attatgccta tggtgccact gggcacccag
      661 gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa ccggaaggat
      721 cctctggggg tggcggtccg tggaggcgaa gcggcggtgg aggttctggt ggtggaggtg
      781 gcacagccat catcaaagag atcgttagca gaaacaaaag gagatatcaa gaggatggat
      841 tcgacttaga cttgacctat atttatccaa acattattgc tatgggattt cctgcagaaa
      901 gacttgaagg cgtatacagg aacaatattg atgatgtagt aaggtttttg gattcaaagc
      961 ataaaaacca ttacaagata tacaatcttt gtgctgaaag acattatgac accgccaaat
     1021 ttaattgcag agttgcacaa tatccttttg aagaccataa cccaccacag ctagaactta
     1081 tcaaaccctt ttgtgaagat cttgaccaat ggctaagtga agatgacaat catgttgcag
     1141 caattcactg taaagctgga aagggacgaa ctggtgtaat gatatgtgca tatttattac
     1201 atcggggcaa atttttaaag gcacaagagg ccctagattt ctatggggaa gtaaggacca
     1261 gagacaaaaa gggagtaact attcccagtc agaggcgcta tgtgtattat tatagctacc
     1321 tgttaaagaa tcatctggat tatagaccag tggcactgtt gtttcacaag atgatgtttg
     1381 aaactattcc aatgttcagt ggcggaactt gcaatcctca gtttgtggtc tgccagctaa
     1441 aggtgaagat atattcctcc aattcaggac ccacacgacg ggaagacaag ttcatgtact
     1501 ttgagttccc tcagccgtta cctgtgtgtg gtgatatcaa agtagagttc ttccacaaac
     1561 agaacaagat gctaaaaaag gacaaaatgt ttcacttttg ggtaaataca ttcttcatac
     1621 caggaccaga ggaaacctca gaaaaagtag aaaatggaag tctatgtgat caagaaatcg
     1681 atagcatttg cagtatagag cgtgcagata atgacaagga atatctagta cttactttaa
     1741 caaaaaatga tcttgacaaa gcaaataaag acaaagccaa ccgatacttt tctccaaatt
     1801 ttaaggtgaa gctgtacttc acaaaaacag tagaggagcc gtcaaatcca gaggctagca
     1861 gttcaacttc tgtaacacca gatgttagtg acaatgaacc tgatcattat agatattctg
     1921 acaccactga ctctgatcca gagaatgaac cttttgatga agatcagcat acacaaatta
     1981 caaaagtctg ataagcggcc gctggccagc tagctaggta gctagatctt tttccctctg
     2041 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2101 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2161 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2221 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     2281 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     2341 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     2401 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     2461 tcttatggag atccctcgac cgatccgctg cattaatgaa tcggccaacg cgcggggaga
     2521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4441 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     4501 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4561 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4621 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4681 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4741 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4801 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4861 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4921 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
//
