LOCUS       sequence-171333-       19815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid 
            vector with double cos sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19815)
  AUTHORS   Jones KM
  TITLE     Increased production of the exopolysaccharide succinoglycan enhances
            Sinorhizobium meliloti 1021 symbiosis with the host plant Medicago 
            truncatula.
  JOURNAL   J Bacteriol. 2012 Aug;194(16):4322-31. doi: 10.1128/JB.00751-12. 
            Epub 2012 Jun 8.
  PUBMED    22685282
REFERENCE   2  (bases 1 to 19815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.00751-12"; journalName: "J Bacteriol"; date: "2012-08"; 
            volume: "194"; issue: "16"; pages: "4322-31"
COMMENT     Sequence Label: pstb-LAFR5
FEATURES             Location/Qualifiers
     source          1..19815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2104..3252
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4525..5724)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             5830..6480
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    6788..7186
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     rep_origin      7364..8075
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     8431..8453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8445..8462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8446..8462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8932..8948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8932..8948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8945..8967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8956..8972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8980..8986,8987..9004,9005..9010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9025..9046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     oriT            17996..18105
                     /note="incP origin of transfer"
     CDS             18138..18509
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ggtgatgtac tggccgttct cgtaaccaag aacttcgaga atttcttgca ctcgacggcc
       61 gctaggggtc ctggcgatat ggacgaccac atgaaccgcc tcgccaatca gcggctcaat
      121 gggtttcggt gaatccgggt gcatgctgat aagcatggcg agccggctca ggcccgcttt
      181 ggggttgttt gcgtgcaggg tggcggcacc tccttcatgc ccggtgttcc aggccatcaa
      241 cagatcaagg gcttcggggc cacgtacctc accgaccagg atgcggtcgg ggcgcatacg
      301 cagcgttgtc ttgagcagca gcgtcatcga gacgtcgatg ctggtgtggt attggacggc
      361 gttctctgcg gcgcactgga tttcgccggt gtcctcgatg atgacgacgc gctcagacgg
      421 gttgaaggcg accatttcat tgatgatcgc gttgacgagc gtggtcttgc ccgagccagt
      481 accgccaatg acgaggatgt ttcgatgcgc cgcgacggcg cttttaatga cctcgtattg
      541 ctcgcgggtc atgatgcccg cctcgacgta ctgttccagc gtgaagatgg cgaccgcgcg
      601 cttgcggatc gcaaaggttg gcgcggccac gaccggcggc aattggccgg caaagcggct
      661 gccatccaag gggaactcgc cttccaggat gggcgaatgc cgcgtgacct ctttgccgtg
      721 gaatccggcc accgtttcta taatcgcctg cgactggctg ggccgcatgt cgcagatgta
      781 ccgcatcggc tcgccaaggc gttcgtgcca caccttgccg tccgcgttga gcatgacttc
      841 aacggttttc gggtcgttga gcgcggccaa caggtccgca cccatgtcgc gttccagctt
      901 gcgcttggcc cgctccttga tggtctgaaa ctcttctttc ccgctcactt ttcagtaacc
      961 cctacgcaac aaatcattag caacattatc gcacaggcaa acgatatacc gaaagtgttt
     1021 agcctgcaag acaaacagct aatcgaaccg cacgacgtag gggttctgat agaaaacaac
     1081 tgtcaaagcg cacccgcccg atgccattcg cggcacggct tccgttgagg atgtcgatat
     1141 gatgcgcgag ccgacggccc gcagagaagg ggccgtttta gcggctaaag aaggaagtgc
     1201 aagccctaac ccttggcgtc agagccttcc acgcagcttt tttcgggtgt cgtcgcccca
     1261 tttctttacg ataaacgcct tatgtgacgg caaaaccaca ctgatgcgtt cgtatccggg
     1321 cggcacgctg ctcttgaaag gatgacccgc aatctccgcg agtgcctcgc ggtcaaggtc
     1381 ggtggactcc aggagaagag gtaggggagt ttccagggcg tcggcaatgg cctccatcac
     1441 cttcaacgag gggttggcct taccgttggt taagtctgat aaaaacgaaa ttgaaacccc
     1501 tgccctctcc gacagctcat gtttcgtcat gccccgctca tcgagcagac gaaggatgtt
     1561 ggtgaaaaat atctggttgt acacagcgga agccgcccct cgcacctttg gtcgcggccc
     1621 gcaaaatttt agccgctaaa gttcttgaca gcggaaccaa tgtttagcta aactagagtc
     1681 tcctttctca aggagacttt cgatatgagc cataatcagt tccagtttat cggtaatctt
     1741 acccgtgaca ccgaggtacg tcatggcaat tctaacaagc cgcaagcaat tttcgatata
     1801 gcggttaatg aagagtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
     1861 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
     1921 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     1981 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     2041 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     2101 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
     2161 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
     2221 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
     2281 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
     2341 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
     2401 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
     2461 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
     2521 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
     2581 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
     2641 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
     2701 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
     2761 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
     2821 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
     2881 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
     2941 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
     3001 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
     3061 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
     3121 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
     3181 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
     3241 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcttta
     3301 ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca gtatcgctcg
     3361 ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg acaattgtga
     3421 ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc gagatccgat
     3481 tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac gaggagaaaa
     3541 agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc gcctacatcg
     3601 acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag gacgctcaca
     3661 aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg gtcgccggta
     3721 tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga cagattccaa
     3781 cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg ctattctgga
     3841 gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg gtaggcgctg
     3901 tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg atacgattga
     3961 tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg ttgacaccaa
     4021 acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt tccatggcgt
     4081 tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc tttaccgcct
     4141 ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt gatccgccaa
     4201 tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg atcggagcgg
     4261 gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca gttgtttcct
     4321 tactgggctt tctcagcccg gggaccgccg tgttgctagg atggttgttc ttggatcaga
     4381 cgctgagtgc gcttcaaatc atcggcgtcc tgctcgtgat cgggagtatc tggctgggcc
     4441 aacgttccaa ccgcactcct agggcgcgta tagcttgccg gaagtcgcct tgacccgcat
     4501 ggcataggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc cgctccaaag
     4561 cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca
     4621 cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat
     4681 cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc
     4741 cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag
     4801 gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt
     4861 gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg
     4921 gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc
     4981 gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg
     5041 cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat
     5101 gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta acggccggcg
     5161 ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa
     5221 ggctgccgcg gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac
     5281 aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg
     5341 ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt
     5401 gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgctg tcgccatgat
     5461 ggcgtagtcg acagtggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg
     5521 atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag
     5581 cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg
     5641 cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac
     5701 gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg
     5761 aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa
     5821 gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg
     5881 aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag
     5941 cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa
     6001 gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca
     6061 tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg
     6121 cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt
     6181 cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc
     6241 agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag
     6301 cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc
     6361 ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga
     6421 ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga
     6481 tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag acaacatccc
     6541 ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct
     6601 gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctcct
     6661 gctgcggcgt ccgctcgtgg gccgtggcgc gggtccgcgc gccggcctcg tgcgcctggc
     6721 gctcgcgggc gaggtccagg gcggccgtct tcacgttctg ccttgcgcag atgagataga
     6781 tccggacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa
     6841 aaagctcgct ttcagcacct gtcgtttcct ttcttttcag agggtatttt aaataaaaac
     6901 attaagttat gacgaagaag aacggaaacg ccttaaaccg gaaaattttc ataaatagcg
     6961 aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg
     7021 ataatgatta tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa
     7081 tcaattatga cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt
     7141 cagacaatac aaatcagcga cactgaatac ggggcaacct catgtccgga tctagcgtgg
     7201 actcaaggct ctcgcgaatg gctcgcgttg gaaactttca ttgacacttg aggggcaccg
     7261 cagggaaatt ctcgtccttg cgagaaccgg ctatgtcgtg ctgcgcatcg agcctgcgcc
     7321 cttggcttgt ctcgcccctc tccgcgtcgc tacggggctt ccagcgcctt tccgacgctc
     7381 accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca
     7441 gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg
     7501 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
     7561 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
     7621 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
     7681 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
     7741 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
     7801 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
     7861 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
     7921 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
     7981 ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc
     8041 ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gcagtccttg ccattgccgg
     8101 gatcggggca gtaacgggat gggcgatcag cccgagcgcg acgcccggaa gcattgacgt
     8161 gccgcaggtg ctggcatcga cattcagcga ccaggtgccg ggcagtgagg gcggcggcct
     8221 gggtggcggc ctgcccttca cttcggccgt cggggcattc acggacttca tggcggggcc
     8281 ggcaattttt accttgggca ttcttggcat agtggtcgcg ggtgccgtgc tcgtgttcgg
     8341 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8401 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     8461 gtgccaagct tggcacgcgt ctgaatgtcc attgttccct cccgaaatcg tgttccccga
     8521 gacagtcctc tctgtcccgg cgggagtatt tctcaaaact cgggtagacg caattatttt
     8581 cagtaaattt ttacctcgtt tttatgagcg gcgacggcgc tttcggccgt ctagcctgca
     8641 caagccatct tgctagcatc gcatgcgtct cctatattgg gggaaaggag tattgaggca
     8701 tgggtgcccc acagctttcg gtccgaagtg caaaagcgcg tgatctggcg cgccgacttg
     8761 cacggcgaga gaaccgctcc atcgctgaca tcgtcgagcg tgctcttgag ttatatgaaa
     8821 ccagagaagc tggccgagag cctgcgagtg ctttctatgc gcgcttgttc gagagttgcg
     8881 gcacggacat cgacctgcag gtcgacggat ccccgggaat tcgtaatcat ggtcatagct
     8941 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9001 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9061 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9121 cgcggggaga ggcggtttgc gtatgggttc gtgcgatccg tctgcatgat ggtgatagcc
     9181 gtcagcatga ttttcgtgtc gtcgaacttg gtgaagggca ttctcggcgg cgatcacgac
     9241 gccggccctg cggagccttc gccgcgtgcg cgattcatgg cggccgtgga ggccaaggat
     9301 ttcgcgcgag tgcaagagct gatcgaggcg cgtggagcca agtcggcggc tgattatgtc
     9361 cttgcgcagc tcgccgtggc cgaaggtctg gaccgcaagc ctggtgcgcg cgtcgtggtc
     9421 gggaaagcgg cgggcagcat ggcaatgccg cctgcggcgc tgggttttac gccaagggga
     9481 gaagcggcat acgccatcga gcggtcagcc tatggtgagc cgaggtccag cattgcgaag
     9541 cagtaccagc aggaatggaa ccggaaggcg gcgacctggt gggcgatggc cggtgtggcc
     9601 ggcatcatcg gcgcgatcct ggcggcggcg gcaaccggct ttgttgggct ggcagtgtcg
     9661 atccgcaacc gagtgaagcg cgtgcgcgac ctgttggtga tggagccggg tgcagagcca
     9721 taagcggcaa gagacgaaag cccggtttcc gggcttttgt tttgttacgc caaggacgag
     9781 ttttagcggc taaaggtgtt gacgtgcgag aaatgtttag ctaaacttct ctcatgtgct
     9841 ggcggctgtc accgctatgt tcaaccaagg cgcggagcaa attatgggtg ttatccatga
     9901 agaaacggct taccgaaagc cagttccagg aggcgatcca ggggctggaa gtggggcagc
     9961 agaccatcga gatagcgcgg ggcgtcttag tcgatgggaa gccacaggcg acgttcgcaa
    10021 cgtcgctggg actgaccagg ggcgcagtgt cgcaagcggt gcatcgcgtg tgggccgcgt
    10081 tcgaggacaa gaacttgccc gaggggtacg cgcgggtaac ggcggttctg ccggaacatc
    10141 aggcgtacat cgtccggaag tgggaagcgg acgccaagaa aaaacaggaa accaaacgat
    10201 gaaaactttg gtcacggcca accagaaagg cggcgtcggc aagacttcga cccttgtgca
    10261 tcttgccttc gactttttcg agcgcggctt gcgggttgcc gtgatcgacc tggaccccca
    10321 gggcaatgcg tcctacacgc tcaaggactt tgctaccggc ctgcatgcaa gcaagctgtt
    10381 cggcgctgtc cctgccggcg gctggaccga aaccgcaccc gcagccggcg acggccaggc
    10441 cgcgcgcctc gccctcatcg agtccaaccc ggtactggcg aacgccgaac ggctgtcgct
    10501 ggacgacgcc cgcgagctgt tcggggcgaa catcaaggcc ctggcgaacc aaggcttcga
    10561 cgtgtgcctg atcgacacgg ccccgaccct tggcgtcggc ctggcggccg ccctcttcgc
    10621 ggccgactat gtgctgtccc ccatcgagct tgaggcgtac agcatccagg gcatcaagaa
    10681 gatggtcacg accattgcga acgtgcgcca gaagaacgcc aagctgcaat tccttggcat
    10741 ggtgcccagc aaggtcgatg cgcggaatcc gcgccacgcg cgccaccaag ccgagctgct
    10801 ggccgcgtac cccaagatga tgattccggc caccgttggc ctgcgcagca gcatcgccga
    10861 tgccctcgca tccggtgtgc cggtctggaa gatcaagaaa acggccgcgc gcaaggcatc
    10921 gaaagaggtt cgcgccctgg ctgattacgt gttcacgaag atggagattt cccaatgact
    10981 gcggctcaag ccaagaccac caagaaaaac accgctgcgg ccgctcagga agccgcaggc
    11041 gcggcgcagc cgtccggcct ggggttggat agcatcggcg acctgtcgag cctcctggac
    11101 gctcctgcgg cgtctcaggg cggttccggc cctatcgagc tggacctgga cctgatcgac
    11161 gaagatccgc atcagccgcg gacggccgac aaccccggct tttccccgga gagcatcgcg
    11221 gaaatcggtg ccacgatcaa agagcgcggg gtgaagtcac ccatttcggt gcgcgagaac
    11281 caggagcagc cgggccgcta tatcatcaat cacggcgccc gccgctaccg tggctcgaag
    11341 tgggccggca agaagtccat cccggcgttc atcgacaacg actacaacga agccgaccag
    11401 gttatcgaga acctgcaacg caacgagctg accccgcgcg aaattgccga cttcattggc
    11461 cgcgagctgg cgaagggcaa gaagaaaggc gatatcgcca aggaaatcgg caagtcgccg
    11521 gcgttcatca cccagcacgt cacgctgctg gacctgccgg agaagatcgc cgatgcgttc
    11581 aacaccggcc gcgtgcgcga cgtgaccgtg gtgaacgagc tggtgacggc cttcaagaag
    11641 cgcccggagg aagtcgaggc gtggcttgac gacgacaccc aggaaatcac gcgcggcacg
    11701 gtcaagctgc tgcgcgagtt cctggacgag aagggccgcg atcccaacac cgtcgatgcc
    11761 ttcaacggcc agactgatgc cgagcgtgac gcggaggccg gcgacggcca ggacggcgag
    11821 gacggcgacc aggacggtaa ggacgccaag gaaaagggcg cgaaggagcc ggacccggac
    11881 aagctgaaaa aggccatcgt ccaggtcgag cacgacgagc gccctgcccg ccttatcctc
    11941 aaccgtcggc cgccggcgga aggctatgcc tggttgaagt acgaggacga cggccaggag
    12001 ttcgaggcga accttgccga cgtgaaactg gtcgcgctca tcgagggctg atccccaaag
    12061 acagcggcgc gggccacccg cgccgcacag acaacggttc cgctacaagg aggaccgaag
    12121 aatgaatccg atgctgttct acatcgcggg aggcgtaggc gcggcgttgc tgctggtttc
    12181 cgcgatcatg ctgttcaagc tgcgcgagcc gaagaaggaa caccgaccgc agcgcaaggc
    12241 ggcggccccg acgccgcagc cggtcgataa cgagctgctg cgcacctttc gccggccgtc
    12301 gcaggccagc caacccgagc catccacgac gccgccggcg cggcgcactg ccccgccgag
    12361 cagcgaacaa tccagaggga atgacggcga tttcgtcacg tcggccctgg tggccggggc
    12421 gaccaacagc accatgctgg gctacctggc cggcggctcg ctgaccgggg ccatgctggg
    12481 cgatgcgctg cggccggata cgccgtctgc ggcgttcgcg gacccctcgc cgtgcttcgg
    12541 cagcagcgac acggacagcg gctggaccga taccggcagc tcttgcgatt tcagttcgtc
    12601 cgacaccgat acgaacaact ggtgagggcg gaccatgact gacgaacaaa agaaacggct
    12661 cctgatgttg caagctatcc ggcgcagcat ccaggcaatg cgcaggggtg tttcggggcc
    12721 ggatagtttc aagggctggt tgaccggcat cggcatcatc gcggcggggt tcctggttgg
    12781 cgagctgctg ccatcccctg cccccggcgt cggcctggtt gtgccagggg cctgcattgc
    12841 cggcttcgtc gtcggctgct ggctcaacag gaaaggccgc tcatggccga cagccattta
    12901 cgaccaattg gcccgttacc agccgctcaa cgagcgggcg taccgcgacc tgcaagagac
    12961 ggtgaaggaa cgcggcctcg actgcgatgc ggtcatggag tggacctaca tcgagcaggc
    13021 cgagttcatg cccctggtgc ccaccaggga ggacctagcg cgcgacaagt tcctgaccgc
    13081 gacgcgggcc gatagggccg aggacgatgc gcgtgcgcgc ttcgtcgtgc tccatcccaa
    13141 gaagcgcggc cccgacgcat gaagaagccc gacgcccgct aatccccttc ccgctgaccc
    13201 tttgcccccg ccgaccgtgc ggggcttttt ttgcctgccc ttcagccgct aaaaaacctg
    13261 ccgttaagtc gagaactgtt cagctaaact cttgcgctga taaggtaggt aagagtatta
    13321 ttattcttac caattaccgg agccaacatg gaactgaaca aagagacgcg cgaccgcatt
    13381 tttgcggctg cggacgagct gttcgagcaa ggcgaccggg agaacttccc gaccgtggat
    13441 gcagtgcgta aggccgcccg cgtcaacatg aacgacgcca gcgccggcat gcgcgaatgg
    13501 cgacgccagc gcacggcgca ggccgccccg ctggcagtcc aggtgccgga cgcggtgcag
    13561 caggccggca accaggccgt ggccgccctc tggcaagccg cccaggccct tgccaatgaa
    13621 tcgttgcagg cagcgcaagc cggctgggat cgtgagcgca acgagttgga agccgtgcgc
    13681 ggggagctgg ccgacgcctt cgaggcgcag gcccgcgagc tggaagaagc ccaggcgcgc
    13741 gttacattgc tagagcagca ggccgccgag gcggccgagc tggccgcacg ccagcggcaa
    13801 gccctggccg aagcacgcac cgccctggca ggagccgagc agcgggccgc gctggcgacg
    13861 cagcgggccg acgaggtgga gcgtcgagcg caggagctgc gcgccgagct ggactacgct
    13921 caccaggacg cccgcgcatt caaagaagag agccgcaaga ccctggacgc ggccaaccag
    13981 gaaatgcagg cgcttcgcgg cgagcgcgac gccaccaggc gcgaagcgga gagtttgcgc
    14041 acggagttgg cagcggtgaa agcgaaggcg caggccgacg ccgaggccca ccaggagcaa
    14101 aggaagttgg cggcgcaaga ggcggccagg caggccgagc gctttacgac ggtgcaagcc
    14161 gagcgtgacg aagcgcggca agaggcaaag gcagcccaag aagaggccgc tatgttgagg
    14221 ggtcggctag acgcggaaac gagtgggagg aaacacaaag cgggaggaaa gtaactgata
    14281 aatcgaacgc agttcactaa aatagatgga acaaccttga cgcgcccggc tctagggaaa
    14341 gccggcctcg aactgccgag gtcggctttt ttttagccgg caagcgacca ggacacgttt
    14401 aacaccagag gcaaccgtca ggagtcaccc aatgaaacac cgcctcctgg tcatgaatgg
    14461 gcaaagaatt gtccagaccg agaaccaagg cgcatggacg aaccagaagg tagataaagc
    14521 cggggccctg aaacctggca tttacaacat ctatatggcc caaaaggccg ataagtcgca
    14581 gcgccacgac ggcagcatcg tccacgcaga tagcggcagc atttaccaac aggtggggaa
    14641 gaactttgta atgcacgccc ggtcagattt cgataaagta cctgaaatcg ggagtgcgaa
    14701 aagcatcacc tatgacgcaa gtggcaaggc acaggtaagt gccgaaagcg tcaagttgag
    14761 tcgcgggcgc actcgttaga gttttagcgg ctaaaaaggg aggcgtcttg agcagctaca
    14821 gcagagccga gcgcgcaccg tggggagact tcccgaaggt ggttcgcaac ggcgaccttg
    14881 gttctttgac caacgagcct gaataccagg ctgcgaagca gggcgacgcg gaagcggcgc
    14941 ttaatttggt cgagcgcctg atttcggacg acactgttgc ccagctcaag acgctgatcg
    15001 gcgacgacaa gccccgcatc gttccagtcc tcgccgtcga ggcggcgggg aacaacaaga
    15061 ttccggccat gatggcggtc gttctggccg accgccttgg ccttgaggtc gaaacggaca
    15121 ttgtgcagcg ggagaaggtc gcccgaaccg gagccggttc agatcatcgc ctggcgttca
    15181 acccgacttt cgagggggag gtgatccccg gccagaagta catcgttgtc gatgacacgc
    15241 tgacgatggg cggcaccatt gcctcattac gcggctacat cgagaacaac ggcggcaagg
    15301 tcatggcggc gtccgtcatg accgcccatg aaggcgcgct tgacctggcg gtcaagccga
    15361 agatgcttgc gggcatcaat gagaaacacg gtccagcaat ggacgcattt tggaaggaaa
    15421 cctttggcta tggcatcgac cgactcaccc aaggagaagc cggacacctc cgcgctgccc
    15481 cgtccgttga cgcaatccga gatcgcatcg ctgcggctcg aaatgaagca gtcaatcgag
    15541 tgggggcaag ccgaactgcg acgacggcgc gagcagggca acagcagtcc ccggcagtaa
    15601 agcagtccag cggcaacagc ggcgaggatt tgctacaggc ggcccaggag gccgagacgg
    15661 aacagcaggc cctcttagaa tccgcgccca tcgagcagac ctaccagcag acccttgcgc
    15721 tctacgtgca ggcaaagcat gaccaggtgg agcgcatcga ggaccgccta gaacagctcg
    15781 tagaccgtca gcaggcgcgt ttgcagcagc tacagtccaa tgcccccggc ttgctgtccc
    15841 tgccgcgtac aaaggccgcc tggcagcagc agcaggccca acagcaagcc cgcttgcaga
    15901 ccctgcacac gcggctggac atggtgcgcg aaatcaagga aggcatgggc attcacgcgc
    15961 ccaagatcga ggaactggcg acgcgcaaga tgcgcgcgga gcatcccgag ctggccggcg
    16021 actgggattc gatgcgtgaa gcagcccgac ggcaccaggc cctgatgcgc aagcaggagc
    16081 aggagaagaa acaggcccag gagcgcgagc gcgggcgcag tcagagcttg ggcctgtcaa
    16141 acaagcccag ctaacgcaaa aacaaagccc ggcaacgccg ggctttttca tctgcgcctc
    16201 tgcgattcat aacgaggccc acaccaccag ggcggccgcg aacaacacca tgccgccggc
    16261 gatcatgttc atcatcgcca cgcgccgcgc gtccgcgacc ttggccgcga gctggcggcc
    16321 aaggccgtcg tcgatttccc tgcggatcgc ttcggccgcc tgcgcggcgc tgtccttcat
    16381 taccttcgcc attgcgtcct tgctggccgc cagggccgcg ttcagcatcc gctccgcttt
    16441 ggccttggcg tcctcgcccc aacgatgggc gatcccttcc agctcttcct tgaacgcggc
    16501 aaggatttcc tcttgcttgg ccgcactgtc ggccatgagc cgggcgttga tggtatgcag
    16561 gatcagcacc gggtcgtcgc ggccgacggc gatgccgtgc ttggccgcaa tctcccggat
    16621 cagctcttca atctggtcgc tcatagcacg gccgccgcgt cgagctgttc aaacaggccg
    16681 cgccgcacga tcttgaggcg ttgccgcgtc atgatcgtga gcgattcatc ggccagcgcc
    16741 tggtcgaacg tcagccgctc ttgcagcatg tcgctgaaat cgcggccgta ggtttcttcc
    16801 ttgagggccg gaatctggat gatggacgac acgcgggcct tgttggccgt gtacgccttc
    16861 atctgctcaa agctcttgcc ctcatgctcg ataggccccc aatacgggtt cagccagacc
    16921 acgaaaagcg cttcggccgg gaactggctg gcgagctggg cgaagccgct caccgtgtcc
    16981 aggagagcct ggccgccggt gacgacggta tggatgacca gctcatgccc catttcttgc
    17041 agcagagccg gcacctggtt gctgatgagg taatgcgaca gaggcacgaa cgagctggca
    17101 ccgttgtcga tcaccacgtc atccttggtc ggcgcaatca gctcgaccag ggtgtcgaag
    17161 ttgcgcgagt taatttcgtc gccggccatg atgttcagcc ggcggacgtt cagggccttg
    17221 tagccctcga acgtcgcgtt caccgggtcg gtgtcgatgc acaagggtgt ctgccccttg
    17281 tccatcttgt actgcgcaat gatcgcggcg atggccgact tgccgacccc gcccttgccc
    17341 tgcaaaacca tgtgaatttt cgccattaca gtagatcctt tttgtccggt gttgggttga
    17401 aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc agccttgccc ccctgcttcg
    17461 gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc cgcagccttg gcctgcggtg
    17521 cgggcacatc ggcgggcttg gccttgatgt gccgcctggc gtgcgagcgg aacgtctcgt
    17581 aggagaactt gaccttcccc gtttcccgca tgtgctccca aatggtgacg agcgcatagc
    17641 cggacgctaa cgccgcctcg acatccgccc tcaccgccag gaacgcaacc gcagcctcat
    17701 cacgccggcg cttcttggcc gcgcgggatt caacccactc ggccagctcg tcggtgtagc
    17761 tctttggcat cgtctctcgc ctgtcccctc agttcagtaa tttcctgcat ttgcctgttt
    17821 ccagtcggta gatattccac aaaacagcag ggaagcagcg cttttccgct gcataaccct
    17881 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
    17941 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
    18001 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
    18061 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa
    18121 cagatgaggg caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta
    18181 tcaaggtgta ctgccttcca gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg
    18241 gcatgagcct gtcggcctac ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg
    18301 tggactatga gcacgtccgc gagctggccc gcatcaatgg cgacctgggc cgcctgggcg
    18361 gcctgctgaa actctggctc accgacgacc cgcgcacggc gcggttcggt gatgccacga
    18421 tcctcgccct gctggcgaag atcgaagaga agcaggacga gcttggcaag gtcatgatgg
    18481 gcgtggtccg cccgagggca gagccatgac ttttttagcc gctaaaacgg ccggggggtg
    18541 cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga agagcgactt cgcggagctg
    18601 gtgaagtaca tcaccgacga gcaaggcaag accgagcgct cttcttcaat cgctgccgtc
    18661 agacggtcag ggaacgccga caggccccgc tccgcgtagt tcggagcagg acgccgcacg
    18721 gcgtccacat ggatcatcca cccacttccc aggcccgcga gggcctggtt gatgcgggcg
    18781 gacactactt cgcgctgctg gtcggtgctg cttgcgttgt catcgccctt gtacagccag
    18841 gcagccataa agctgccgtt cttgcccacg attacgccgt catcgacgac agcggcgtag
    18901 ttgagcagat cggccaggcc ggcgtccttg gaacgatgct ttttcagttt cagttcggca
    18961 tcgaccgcgc ggatgcgggc aaagaggatg aacaacagaa gcgcgccgag gcccgcgatt
    19021 gcaatcgcaa ttgcttggat catcggtatt gcttcccttg gctattggtg ttctcgcgga
    19081 acggggtcga gcgggccggg taatacggct tgtaccggcg gtgacgcagg tacacgaacc
    19141 gcatcttcgg atcggccttc gccatgattc ggaacgcata gagcgccccg aaccacagga
    19201 tcagaccgac cacggtggcc cgcagctctt gggcgctgaa aatcagcgca aacgccatca
    19261 ggcccgagaa catcaccagt tcacgatcac cacccatgaa caggttttct cggttgcctg
    19321 cgcgacggat ggggatcgtg cgcagagcca tgattaggcg agccgtccag ccgctaccgc
    19381 acgcacggca tccgccgccg cgacttgcac ctggtgcagc gccccgttgc cgagggccgc
    19441 gatttcggca ccacgaccga agaaggtgct catcacgttc tgcgcgccga ccagcagcgc
    19501 catcaccaga accaggaaga tcagggttcg gaagaaggcg ttgagttcgc cgccgaagat
    19561 cagcacgccg ccggcgacga cgatgccgat gatggacagc gcgaaggcca ccgggccggt
    19621 tacggagttg cgcaggttcg tcagccagct ctcatatggc aagctgccgc cggtgccttc
    19681 cgaggccatc gccggatgcg cggataacgc gagagcgaga acgaagaaca cggcaaggta
    19741 gaacaaaatg ccgcgattca tggtcagacg gaacggaaca gccgttgtca ttggaaatac
    19801 tccttacagg gtttt
//
