LOCUS       sequence-171337-        9136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, 
            Garcia BA, Tuschl T, Stadtfeld M, Reinberg D
  TITLE     Co-repressor CBFA2T2 regulates pluripotency and germline 
            development.
  JOURNAL   Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.
  PUBMED    27281218
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-8- 8"; volume: "534"; issue: "7607"; pages:
            "387-90"
COMMENT     Sequence Label: mCBFKOgRNA5
FEATURES             Location/Qualifiers
     source          1..9136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          30..257
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             278..343
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             350..370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             395..4495
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             395..4495
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4496..4543
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4559..4612
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4613..4615,4616..5326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4613..4615,4616..5326)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4916..4935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5263..5284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5354..5371)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5360..5567
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5576..5716
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5576..5705
                     /label=AAV2 ITR
     rep_origin      5791..6246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5878..5897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6088..6109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6263..6282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6382..6404
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6442..6460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6528..6632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6633..6701,6702..7493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6851..6870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7664..8252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8153..8172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8314..8562
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8314..8334
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8485..8504
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8584..8659
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8756..9041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
       61 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
      121 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagctgagc aagaggtaag
      181 ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag cacctgcctg
      241 aaatcacttt ttttcaggtt ggaccggtgc caccatggac tataaggacc acgacggaga
      301 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
      361 gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca tcggcctgga
      421 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      481 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      541 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      601 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      661 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      721 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
      781 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
      841 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
      901 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
      961 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     1021 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     1081 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     1141 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     1201 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     1261 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     1321 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     1381 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     1441 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     1501 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     1561 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     1621 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     1681 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     1741 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     1801 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     1861 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     1921 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     1981 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     2041 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     2101 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     2161 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     2221 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     2281 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     2341 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     2401 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     2461 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     2521 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     2581 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     2641 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     2701 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     2761 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     2821 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     2881 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     2941 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     3001 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     3061 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     3121 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     3181 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     3241 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     3301 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     3361 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     3421 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     3481 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     3541 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     3601 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     3661 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     3721 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     3781 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     3841 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     3901 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     3961 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     4021 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     4081 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     4141 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     4201 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     4261 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     4321 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     4381 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     4441 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaaaag
     4501 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggaattcg gcagtggaga
     4561 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa
     4621 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4681 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4741 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4801 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4861 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4921 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4981 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5041 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5101 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5161 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5221 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5281 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaat tctaactaga
     5341 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5401 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5461 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5521 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgcaggaa
     5581 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     5641 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     5701 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5761 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     5821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     5881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6001 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6121 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     6181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     6241 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6301 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6361 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6421 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6481 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6601 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6661 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6721 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6781 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6841 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6901 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6961 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7021 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7081 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7141 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7201 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7261 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7321 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
     7381 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7441 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7501 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7561 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7621 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7681 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7741 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7801 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7861 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7921 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7981 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8041 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8101 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8161 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8221 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8281 tcctggcctt ttgctggcct tttgctcaca tgtgagggcc tatttcccat gattccttca
     8341 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     8401 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     8461 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     8521 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgctggccc ccaggattca
     8581 taagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     8641 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     8701 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacccgtta
     8761 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8821 caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     8881 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     8941 atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta
     9001 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     9061 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     9121 tttaattatt ttgtgc
//
