LOCUS       sequence-171360-        2326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA tagged human CBFA2T2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Tu S, Narendra V, Yamaji M, Vidal SE, Rojas LA, Wang X, Kim SY, 
            Garcia BA, Tuschl T, Stadtfeld M, Reinberg D
  TITLE     Co-repressor CBFA2T2 regulates pluripotency and germline 
            development.
  JOURNAL   Nature. 2016 Jun 8;534(7607):387-90. doi: 10.1038/nature18004.
  PUBMED    27281218
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-8- 8"; volume: "534"; issue: "7607"; pages:
            "387-90"
COMMENT     Sequence Label: CAGhCBFA2T2
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     297..319
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     381..400
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      434..443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             443..466
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2279..2305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
       61 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
      121 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
      181 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
      241 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
      301 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
      361 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
      421 agaattcctc gaggccacca tggactacaa agacgatgac gacaagatgg ctaaagaatc
      481 tggaataagc ttgaaagaaa tacaggtcct ggcaaggcaa tggaaagttg gtcctgagaa
      541 aagggtgcca gcgatgcctg gatcgcctgt ggaagtgaag atacagtcca gatcctcacc
      601 tcccaccatg ccacccctcc caccaataaa tcctggagga ccgaggccag tgtccttcac
      661 tcctactgca ttaagcaatg gcatcaacca ttctcctcct accctgaatg gtgccccatc
      721 accgccacag agattcagca atggtcctgc ctcctccaca tcatctgcac tcacaaatca
      781 gcaattgcca gccacttgtg gtgctcgaca actcagcaag ttgaaacgct ttcttaccac
      841 tctgcaacag tttggcaatg acatctcccc tgagattggg gagaaggtgc ggactcttgt
      901 tcttgcactg gtgaactcaa cagtgacaat tgaggaattc cactgtaagc tccaagaagc
      961 cacaaacttt ccccttcgtc cttttgtgat tccatttctc aaggccaacc tgcccctgct
     1021 gcagcgggaa ctgctgcact gcgctcgggc ggccaagcag accccatccc agtacctggc
     1081 tcagcacgaa caccttctgc tcaacacaag cattgcatcg cctgctgact cgtcagagtt
     1141 gctcatggag gtgcacggaa atgggaagag gcccagtcca gagaggagag aagagaatag
     1201 ttttgataga gacacaattg ctcctgagcc tcctgccaag agagtatgta ccatcagccc
     1261 tgctcctcgg cacagtcctg ctctcactgt gcccctcatg aatcctgggg gccaattcca
     1321 tcctacccct ccacctcttc agcattacac cttagaggat attgcaactt ctcacctgta
     1381 tcgggaaccc aacaagatgc tagagcatcg agaagttcgt gatagacacc acagtcttgg
     1441 tctaaatgga ggctatcaag atgagttggt agatcatcgt ttgacagaaa gggaatgggc
     1501 tgatgaatgg aaacatcttg accatgcgct gaattgcatt atggaaatgg tagagaaaac
     1561 aaggcgctct atggcagttc tgcggcgctg tcaggaatca gatcgtgaag aactcaacta
     1621 ctggaaaaga cggtacaatg aaaacacaga gctgaggaaa acggggaccg agttggtctc
     1681 caggcagcac agccctggga gtgcagattc tctcagcaat gattctcaga gagagttcaa
     1741 cagcaggcca ggtacaggat acgtacctgt ggagttttgg aaaaaaacag aagaagctgt
     1801 gaataaggtg aaaattcagg ccatgtcaga agtacagaag gccgtcgctg aggcagagca
     1861 gaaagccttt gaagtgattg caacagagag agcacgaatg gagcaaacca tagcggatgt
     1921 caagcggcag gccgcagagg atgctttcct cgtcatcaat gagcaagagg agtccacgga
     1981 gaactgctgg aactgtggcc gcaaagccag cgagacatgc agtggctgca atatcgcgcg
     2041 atactgtggc tctttctgcc agcacaagga ctgggagcgg caccaccgcc tctgtggtca
     2101 gaacctgcat ggccagagcc cccacggcca gggccggccg ctgcttcctg taggcagggg
     2161 ctcctctgcc aggtccgccg actgcagcgt gcccagccca gccctcgaca agacctcggc
     2221 aaccacatcg cgttcctcaa cacctgcttc tgtgacagct atcgacacca acggactcta
     2281 cccatacgat gttccagatt acgcttaggc ggccgctcga taagct
//
