LOCUS       sequence-171363-        7193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Uchida A, Alami NH, Brown A
  TITLE     Tight functional coupling of kinesin-1A and dynein motors in the 
            bidirectional transport of neurofilaments.
  JOURNAL   Mol Biol Cell. 2009 Dec . 20(23):4997-5006.
  PUBMED    19812246
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2009 Dec . 20(23):4997-5006."
COMMENT     Sequence Label: pKin1B
FEATURES             Location/Qualifiers
     source          1..7193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             146..940
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             146..940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(200..219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     810..829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1128..1147)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1172..1219
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1548..2136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2037..2056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2615..2818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2769..2789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    6311..6432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6348..6367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6402..6421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6439..6894)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6576..6597)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6788..6807
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6921..7025
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7050..7070)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
       61 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
      121 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      301 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
      361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      661 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
      781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
      841 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
      901 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
      961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1021 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1081 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     1141 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     1201 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     1261 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     1321 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     1381 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2221 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2641 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2701 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2761 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2821 tttagtgaac cgtcagatcc gctagcgcta ccggtagtga acttcgggcg caggctgccg
     2881 gcgacagcgc ttcggtctag ctgcccgggg tgcacggcgc tgcggagccc ggaccttccg
     2941 gtcctcgcga cccctgcctc ttggctcccc tcgcttaccc caagactgag cggcttgaag
     3001 aggcgcccgg cctgcggtcc cggcttccag aaagatggcg gacccggcgg agtgcaacat
     3061 caaagtgatg tgtcgcttca gacctctcaa cgaatctgaa gtgaaccgcg gcgataagta
     3121 cgtcgccaaa ttccagggag aagacacggt gatgatcgcg tccaagcctt atgcctttga
     3181 tcgtgtgttc cagtcaagca catctcaaga gcaagtatac aacgactgtg caaaaaagat
     3241 tgttaaagat gttcttgagg gctataatgg aacaatattt gcatatggac aaacatcatc
     3301 tgggaagacc cacacgatgg agggtaaact tcatgatcca gaaggcatgg gaattattcc
     3361 aagaatagtg caagatattt ttaattatat ttactccatg gatgaaaatt tggaatttca
     3421 tattaaggtt tcatattttg aaatatattt ggataagata agggacttgt tagatgtttc
     3481 aaagactaac ctttcagtcc atgaagacaa aaaccgtgtt ccctatgtaa aggggtgcac
     3541 agaacgtttc gtgtgtagtc cagatgaagt catggatacc atagatgaag ggaaatccaa
     3601 cagacatgtc gcagttacaa atatgaatga acatagctct aggagtcaca gcatatttct
     3661 tattaatgta aaacaagaga atacacaaac ggaacagaaa ctcagtggaa agctttatct
     3721 ggttgattta gctggcagtg agaaggttag taagactggg gctgaaggtg ctgtgctgga
     3781 tgaagctaag aacatcaaca agtcactttc tgcacttgga aatgtcattt ctgctttggc
     3841 agagggcagt acctatgttc cttatcgaga tagtaaaatg accagaattc ttcaagattc
     3901 attaggtggc aactgtagga ccactattgt catatgctgc tctccatcat catacaatga
     3961 gtctgagaca aagtcaacac tcctctttgg tcaaagggcc aaaacaatta agaacacagt
     4021 ctgtgtcaat gtagagttaa ctgcggagca gtggaaaaag aagtatgaaa aagaaaagga
     4081 aaaaaataaa actctacgga acactattca gtggctggaa aacgagctaa accgttggcg
     4141 taacggggag acagtgccta ttgatgagca gtttgacaaa gagaaagcta atttggaagc
     4201 cttcacagcg gataaagata ttgctattac cagtgataaa ccagctgctg cagtcggaat
     4261 ggctggtagt tttaccgatg ctgaaagaag aaagtgtgaa gaagaacttg ctaaattgta
     4321 taaacaactt gatgacaagg atgaagagat taaccaacaa agccaattgg tagagaaatt
     4381 gaagacacaa atgctggatc aggaagagct tctggcatca accagaaggg atcaagataa
     4441 tatgcaagct gaactgaatc gcctccaagc agaaaatgat gcttctaaag aagaagtcaa
     4501 agaagtttta caggccttag aggaactggc tgttaattat gatcagaagt ctcaggaagt
     4561 tgaagacaaa acaaaggaat atgaattgct tagtgatgaa ttgaatcaaa aatctgcaac
     4621 tttagcaagt attgatgctg agcttcagaa gctgaaggaa atgaccaacc accagaagaa
     4681 acgagcagct gaaatgatgg catcattatt aaaagacctt gcagaaatag gaattgctgt
     4741 ggggaataac gatgtgaagc aaccagaagg aactggtatg atagatgaag agtttactgt
     4801 tgcaagactc tacattagca aaatgaaatc agaagtaaag accatggtga aacgctgcaa
     4861 acagctagaa agcacacaga ctgagagcaa caaaaaaatg gaagaaaatg agaaagagtt
     4921 agcagcatgc cagcttcgga tctcccaaca tgaagccaaa atcaagtcac tgactgagta
     4981 ccttcagaat gtagaacaaa agaagaggca gctggaggag tctgttgatt cccttggtga
     5041 ggagctagtc caactccgag cacaagagaa agtccatgaa atggaaaaag agcacttgaa
     5101 caaggttcag actgcaaatg aagtcaagca agctgttgag cagcagatcc agagtcacag
     5161 agaaacccac caaaaacaaa tcagtagttt gcgagatgaa gtggaggcaa aggaaaagct
     5221 aatcactgac ctccaagacc aaaaccagaa gatggtgttg gagcaggaac ggctaagggt
     5281 ggagcatgag aggctgaagg ctacagacca agagaagagc aggaagctgc acgagctcac
     5341 ggttatgcaa gacagacgag aacaagcaag acaagacttg aagggtttgg aggagaccgt
     5401 ggcaaaagaa cttcagactt tacacaacct gcgtaagctc tttgttcagg acttggctac
     5461 cagggtgaaa aagagcgccg aggtcgactc tgacgacact ggcggcagtg ctgcacagaa
     5521 gcagaaaatc tccttccttg aaaacaacct tgaacagctc accaaagtgc acaagcagtt
     5581 ggtacgcgat aatgcagatc ttcgctgtga gcttcctaag ttagagaaac ggcttagagc
     5641 tactgcagaa agagtgaaag ctttggagtc agccctgaaa gaagccaaag aaaatgcatc
     5701 tcgagaccgt aaacgctatc agcaagaagt agaccggata aaggaagcag tcaggtcaaa
     5761 gaacatggcc agaaggggac attctgccca gattgcaaag ccgatccgtc ctggacagca
     5821 tccagcggcc tcgccaactc acccgggcac agttcgtgga ggaggctcat ttgttcagaa
     5881 caaccagcca gtggggcttc gtggtggtgg aggcaagcag tcgtaaacac ttgacacatt
     5941 gcaggtgttg aaaagtaatt gaagtttgaa gaggacataa aatcagtctt tcactaacta
     6001 acctctattc cttgggattg tagaaattta attttttaat gtgtaaatta ctcctaccct
     6061 gtacagctgt ttcctaccca ctcttgtaaa ctctgctgct tcccaacact agagtgcaat
     6121 gttgatgtcc tagaagggaa acgacagttt acaactgcgg ccctgtttgt tacacggtgt
     6181 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     6241 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     6301 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6361 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6421 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     6481 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     6541 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     6601 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     6661 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     6721 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     6781 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6841 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     6901 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6961 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7021 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7081 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7141 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atc
//
