LOCUS       sequence-171373-       10095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce lentiviral vector for HTT editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..10095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    678..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1715..1759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2026..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2179..2203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2229..2443
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2364..2383
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        2470..2545
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(2572..2596)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2619..2635)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2620..2636)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2652..3151
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2687..2710)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3084..3103
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3166..3175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3172..3882
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3317..3335)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3585..3604
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3978..4566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4031..4051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4449..4460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4655..4888
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4913..4931)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4914..4931)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5221..5240)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5455..5474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5574..5596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5634..5652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5720..5824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5825..5893,5894..6685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6043..6062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6856..7444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7345..7364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7598..7615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7685..7705)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7690..8019
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7870..8005
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9153..9218
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9348..9368
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9793..9927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9830..9849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9884..9903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagagctc tctcgacgca ggactcggct tgctgaagcg cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cctcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattg
     1621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1921 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1981 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgcta gtggatcccc
     2161 cgggctgcag gaattatcac aagtttgtac aaaaaagcag gcttcatcga tcaattggga
     2221 tccaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
     2281 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
     2341 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
     2401 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatctg accctggaaa
     2461 agctgatgag ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     2521 tgaaaaagtg gcaccgagtc ggtgcttttt tagatcggat ccaagctcct gacccagctt
     2581 tcttgtacaa agtggtgata attcgatatc aagcttatcg ataccgtcga cctcgacgat
     2641 ggtcgagtac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2701 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2761 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2821 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2881 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2941 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     3001 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     3061 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3121 gttctcctct tcctcatctc cgggcctttc gacctctagg atcccgccac catggtgagc
     3181 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     3241 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3301 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     3361 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     3421 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3481 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     3541 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     3601 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3661 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3721 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     3781 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3841 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgtcgagaga
     3901 tgggggaggc taactgagct agcttagtca tgcaccgggc tgcaggaatt cgcggccgca
     3961 ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4381 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4561 ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     4621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4681 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4861 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     4921 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     4981 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     5041 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     5101 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     5161 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     5221 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     5281 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     5341 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     5401 gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc tcagtacaat
     5461 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5521 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5581 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5641 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5701 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5761 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5821 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5881 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5941 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6001 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6061 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6361 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6961 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7261 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7561 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7621 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7681 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7741 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7861 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7921 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7981 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     8041 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     8101 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     8161 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     8221 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     8281 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     8341 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     8401 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     8461 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     8521 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     8581 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     8641 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     8701 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     8761 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     8821 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     8881 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     8941 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     9001 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     9061 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     9121 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     9181 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     9241 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     9301 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     9361 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     9421 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     9481 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     9541 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     9601 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     9661 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagagctt
     9721 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9781 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9901 atcaatgtat cttatcatgt gtggatcaac tggataactc aagctaacca aaatcatccc
     9961 aaacttccca ccccataccc tattaccact gccaaattac ctgtggtttc atttactcta
    10021 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    10081 tacaaactta gtagt
//
