LOCUS       sequence-171379-       11733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Chen YN, LaMarche MJ, Chan HM, Fekkes P, Garcia-Fortanet J, Acker 
            MG, Antonakos B, Chen CH, Chen Z, Cooke VG, Dobson JR, Deng Z, Fei 
            F, Firestone B, Fodor M, Fridrich C, Gao H, Grunenfelder D, Hao HX, 
            Jacob J, Ho S, Hsiao K, Kang ZB, Karki R, Kato M, Larrow J, La Bonte
            LR, Lenoir F, Liu G, Liu S, Majumdar D, Meyer MJ, Palermo M, Perez 
            L, Pu M, Price E, Quinn C, Shakya S, Shultz MD, Slisz J, Venkatesan 
            K, Wang P, Warmuth M, Williams S, Yang G, Yuan J, Zhang JH, Zhu P, 
            Ramsey T, Keen NJ, Sellers WR, Stams T, Fortin PD
  TITLE     Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by
            receptor tyrosine kinases.
  JOURNAL   Nature. 2016 Jul 7;535(7610):148-52.
  PUBMED    27362227
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2016-07-7"; volume: "535(7610)"; pages: "148-52"
COMMENT     Sequence Label: SHP2(WT)@pLKO-Trex-SBP
FEATURES             Location/Qualifiers
     source          1..11733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(152..169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     379..398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             565..1359
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(619..638)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1229..1248
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1520..1753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1825..1946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1862..1881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1916..1935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1986..2121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2142..2160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2170..2187)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2170..2186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2179..2201)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2328..2783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2415..2434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2625..2646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2809..2913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2914..2982,2983..3774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3132..3151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3945..4533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4434..4453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4687..4704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4821..4842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4857..4862,4863..4880,4881..4887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4895..4911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4900..4922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4919..4935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4919..4935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4954..4974
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4956..4974
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5002..5228
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5229..5409
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5456..5581
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6032..6265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6450..6494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6792..6909
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6992..7371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7372..7575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7526..7546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7577..7595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7598..7616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7626..7650
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7682..7795
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             7796..7819
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    7832..7856
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9642..9666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9722..9839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9888..10398
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10317..10335
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          10407..10979
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     10987..11006
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        11034..11052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11063..11683
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(11136..11155)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    join(11718..11733,1..558)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
       61 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      241 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
      301 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
      361 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
      421 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
      481 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
      541 tgaaaaacac gatgataagg atccatggct gaacaagatg gattgcacgc aggttctccg
      601 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      661 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      721 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      781 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      841 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      901 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      961 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     1021 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     1081 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     1141 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1201 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1261 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1321 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa cctggtgcat gacccgcaag
     1381 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     1441 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     1501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     1561 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1741 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     1801 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     1861 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1921 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     1981 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2101 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     2161 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2221 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2281 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     2341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2401 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2521 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2641 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2701 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2761 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     2821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2881 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2941 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3001 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3061 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3121 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3181 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3241 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3301 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3361 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3421 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3481 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3541 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3601 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3661 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3721 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3781 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4921 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     4981 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     5041 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     5101 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     5161 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     5221 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5281 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5341 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5401 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     5461 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     5521 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     5581 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     5641 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     5701 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     5761 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     5821 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5881 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgcgaagt gaattatata
     5941 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     6001 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     6061 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     6121 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     6181 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     6241 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     6301 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     6361 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     6421 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     6481 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     6541 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     6601 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     6661 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     6721 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     6781 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6841 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6901 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     6961 ctctagaatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     7021 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7081 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     7141 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     7201 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     7261 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     7321 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     7381 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     7441 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     7501 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     7561 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
     7621 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttaccgg
     7681 tatggacgag aagaccaccg gctggcgggg cggccacgtg gtggagggcc tggccggcga
     7741 gctggagcag ctgcgggcca ggctggagca ccaccctcag ggccagcggg agcccgatta
     7801 caaggacgac gacgataaga cgcgtcgatc aacaagtttg tacaaaaaag caggctcgat
     7861 gacatcgcgg agatggtttc acccaaatat cactggtgtg gaggcagaaa acctactgtt
     7921 gacaagagga gttgatggca gttttttggc aaggcctagt aaaagtaacc ctggagactt
     7981 cacactttcc gttagaagaa atggagctgt cacccacatc aagattcaga acactggtga
     8041 ttactatgac ctgtatggag gggagaaatt tgccactttg gctgagttgg tccagtatta
     8101 catggaacat cacgggcaat taaaagagaa gaatggagat gtcattgagc ttaaatatcc
     8161 tctgaactgt gcagatccta cctctgaaag gtggtttcat ggacatctct ctgggaaaga
     8221 agcagagaaa ttattaactg aaaaaggaaa acatggtagt tttcttgtac gagagagcca
     8281 gagccaccct ggagattttg ttctttctgt gcgcactggt gatgacaaag gggagagcaa
     8341 tgacggcaag tctaaagtga cccatgttat gattcgctgt caggaactga aatacgacgt
     8401 tggtggagga gaacggtttg attctttgac agatcttgtg gaacattata agaagaatcc
     8461 tatggtggaa acattgggta cagtactaca actcaagcag ccccttaaca cgactcgtat
     8521 aaatgctgct gaaatagaaa gcagagttcg agaactaagc aaattagctg agaccacaga
     8581 taaagtcaaa caaggctttt gggaagaatt tgagacacta caacaacagg agtgcaaact
     8641 tctctacagc cgaaaagagg gtcaaaggca agaaaacaaa aacaaaaata gatataaaaa
     8701 catcctgccc tttgatcata ccagggttgt cctacacgat ggtgatccca atgagcctgt
     8761 ttcagattac atcaatgcaa atatcatcat gcctgaattt gaaaccaagt gcaacaattc
     8821 aaagcccaaa aagagttaca ttgccacaca aggctgcctg caaaacacgg tgaatgactt
     8881 ttggcggatg gtgttccaag aaaactcccg agtgattgtc atgacaacga aagaagtgga
     8941 gagaggaaag agtaaatgtg tcaaatactg gcctgatgag tatgctctaa aagaatatgg
     9001 cgtcatgcgt gttaggaacg tcaaagaaag cgccgctcat gactatacgc ttcgggagct
     9061 aaaactttca aaggttggac aagggaatac ggagagaacg gtctggcaat accactttcg
     9121 gacctggccg gaccacggcg tgcccagcga ccctgggggc gtgctggact tcctggagga
     9181 ggtgcaccat aagcaggaga gcatcatgga tgcagggccg gtcgtggtgc actgcagtgc
     9241 tggaattggc cggacaggga cgttcattgt gattgatatt cttattgaca tcatcagaga
     9301 gaaaggtgtt gactgcgata ttgacgttcc caaaaccatc cagatggtgc ggtctcagag
     9361 gtcagggatg gtccagacag aagcacagta ccgatttatc tatatggcgg tccagcatta
     9421 tattgaaaca ctacagcgca ggattgaaga agagcagaaa agcaagagga aagggcacga
     9481 atatacaaat attaagtatt ctctagcgga ccagacgagt ggagatcaga gccctctccc
     9541 gccttgtact ccaacgccac cctgtgcaga aatgagagaa gacagtgcta gagtctatga
     9601 aaacgtgggc ctgatgcaac agcagaaaag tttcagatga gacccagctt tcttgtacaa
     9661 agtggttgat taattaaggg gaattctcga cctcgagaca aatggcagta ttcatccaca
     9721 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9781 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9841 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
     9901 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     9961 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
    10021 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
    10081 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
    10141 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
    10201 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
    10261 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
    10321 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
    10381 accgacctct ctccccaggg ggatctgtga gtttggggac ccttgattgt tctttctttt
    10441 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
    10501 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
    10561 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
    10621 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
    10681 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
    10741 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
    10801 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca
    10861 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
    10921 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
    10981 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg
    11041 actcactata gggcgaattg atatgtctag attagataaa agtaaagtga ttaacagcgc
    11101 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
    11161 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
    11221 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
    11281 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
    11341 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
    11401 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
    11461 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
    11521 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
    11581 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
    11641 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gcggctcccg
    11701 ggagttctag ggatctgccc ctctccctcc ccc
//
