LOCUS       sequence-171380-       11733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHP2 mutant gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Chen YN, LaMarche MJ, Chan HM, Fekkes P, Garcia-Fortanet J, Acker 
            MG, Antonakos B, Chen CH, Chen Z, Cooke VG, Dobson JR, Deng Z, Fei 
            F, Firestone B, Fodor M, Fridrich C, Gao H, Grunenfelder D, Hao HX, 
            Jacob J, Ho S, Hsiao K, Kang ZB, Karki R, Kato M, Larrow J, La Bonte
            LR, Lenoir F, Liu G, Liu S, Majumdar D, Meyer MJ, Palermo M, Perez 
            L, Pu M, Price E, Quinn C, Shakya S, Shultz MD, Slisz J, Venkatesan 
            K, Wang P, Warmuth M, Williams S, Yang G, Yuan J, Zhang JH, Zhu P, 
            Ramsey T, Keen NJ, Sellers WR, Stams T, Fortin PD
  TITLE     Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by
            receptor tyrosine kinases.
  JOURNAL   Nature. 2016 Jul 7;535(7610):148-52.
  PUBMED    27362227
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2016-07-7"; volume: "535(7610)"; pages: "148-52"
COMMENT     Sequence Label: SHP2(T253M/Q257L)@pLKO-Trex-SBP
FEATURES             Location/Qualifiers
     source          1..11733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..579
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(173..190)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     400..419
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             586..1380
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(640..659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1250..1269
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1541..1774
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1846..1967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1883..1902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1937..1956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2007..2142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2163..2181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2191..2208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2191..2207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2200..2222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2349..2804
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2436..2455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2646..2667
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2830..2934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2935..3003,3004..3795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3153..3172)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3966..4554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4455..4474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4708..4725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4842..4863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4878..4883,4884..4901,4902..4908)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4916..4932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4921..4943
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4940..4956
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4940..4956
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4975..4995
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4977..4995
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5023..5249
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5250..5430
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5477..5602
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6053..6286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6471..6515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6813..6930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7013..7392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7393..7596
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7547..7567
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7598..7616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7619..7637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7647..7671
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7703..7816
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             7817..7840
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    7853..7877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9663..9687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9743..9860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9909..10419
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10338..10356
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          10428..11000
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     11008..11027
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        11055..11073
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11084..11704
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(11157..11176)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atctgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
       61 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      121 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      181 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
      241 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
      301 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
      361 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
      421 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
      481 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
      541 cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccatggc tgaacaagat
      601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
      781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     1021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     1081 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     1141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     1201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1381 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     1441 accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca
     1501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     1561 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     1621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1741 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     1801 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     1861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     1981 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2041 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2101 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     2161 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     2221 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2281 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2341 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2761 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2821 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2881 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2941 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3001 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3061 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3121 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3181 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3241 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3301 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3361 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3421 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3481 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3541 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3601 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3661 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3721 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3781 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4621 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4681 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4741 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4801 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4861 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4921 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     4981 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     5041 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     5101 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     5161 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     5221 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     5281 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5341 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5401 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     5461 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     5521 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     5581 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     5641 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     5701 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     5761 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     5821 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     5881 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     5941 ggccgcgaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     6241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     6301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     6361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     6421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     6481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     6541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     6601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     6661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     6721 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     6781 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     6841 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     6901 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     6961 gtttggttag taccgggccc gctctagaat gtacgggcca gatatacgcg ttgacattga
     7021 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7081 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     7141 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     7201 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     7261 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     7321 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7381 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     7441 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     7501 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7561 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
     7621 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
     7681 cgccatccac gctgttaccg gtatggacga gaagaccacc ggctggcggg gcggccacgt
     7741 ggtggagggc ctggccggcg agctggagca gctgcgggcc aggctggagc accaccctca
     7801 gggccagcgg gagcccgatt acaaggacga cgacgataag acgcgtcgat caacaagttt
     7861 gtacaaaaaa gcaggctcga tgacatcgcg gagatggttt cacccaaata tcactggtgt
     7921 ggaggcagaa aacctactgt tgacaagagg agttgatggc agttttttgg caaggcctag
     7981 taaaagtaac cctggagact tcacactttc cgttagaaga aatggagctg tcacccacat
     8041 caagattcag aacactggtg attactatga cctgtatgga ggggagaaat ttgccacttt
     8101 ggctgagttg gtccagtatt acatggaaca tcacgggcaa ttaaaagaga agaatggaga
     8161 tgtcattgag cttaaatatc ctctgaactg tgcagatcct acctctgaaa ggtggtttca
     8221 tggacatctc tctgggaaag aagcagagaa attattaact gaaaaaggaa aacatggtag
     8281 ttttcttgta cgagagagcc agagccaccc tggagatttt gttctttctg tgcgcactgg
     8341 tgatgacaaa ggggagagca atgacggcaa gtctaaagtg acccatgtta tgattcgctg
     8401 tcaggaactg aaatacgacg ttggtggagg agaacggttt gattctttga cagatcttgt
     8461 ggaacattat aagaagaatc ctatggtgga aacattgggt acagtactac aactcaagca
     8521 gccccttaac acgactcgta taaatgctgc tgaaatagaa agcagagttc gagaactaag
     8581 caaattagct gagaccacag ataaagtcaa acaaggcttt tgggaagaat ttgagatgct
     8641 acaacaattg gagtgcaaac ttctctacag ccgaaaagag ggtcaaaggc aagaaaacaa
     8701 aaacaaaaat agatataaaa acatcctgcc ctttgatcat accagggttg tcctacacga
     8761 tggtgatccc aatgagcctg tttcagatta catcaatgca aatatcatca tgcctgaatt
     8821 tgaaaccaag tgcaacaatt caaagcccaa aaagagttac attgccacac aaggctgcct
     8881 gcaaaacacg gtgaatgact tttggcggat ggtgttccaa gaaaactccc gagtgattgt
     8941 catgacaacg aaagaagtgg agagaggaaa gagtaaatgt gtcaaatact ggcctgatga
     9001 gtatgctcta aaagaatatg gcgtcatgcg tgttaggaac gtcaaagaaa gcgccgctca
     9061 tgactatacg cttcgggagc taaaactttc aaaggttgga caagggaata cggagagaac
     9121 ggtctggcaa taccactttc ggacctggcc ggaccacggc gtgcccagcg accctggggg
     9181 cgtgctggac ttcctggagg aggtgcacca taagcaggag agcatcatgg atgcagggcc
     9241 ggtcgtggtg cactgcagtg ctggaattgg ccggacaggg acgttcattg tgattgatat
     9301 tcttattgac atcatcagag agaaaggtgt tgactgcgat attgacgttc ccaaaaccat
     9361 ccagatggtg cggtctcaga ggtcagggat ggtccagaca gaagcacagt accgatttat
     9421 ctatatggcg gtccagcatt atattgaaac actacagcgc aggattgaag aagagcagaa
     9481 aagcaagagg aaagggcacg aatatacaaa tattaagtat tctctagcgg accagacgag
     9541 tggagatcag agccctctcc cgccttgtac tccaacgcca ccctgtgcag aaatgagaga
     9601 agacagtgct agagtctatg aaaacgtggg cctgatgcaa cagcagaaaa gtttcagatg
     9661 agacccagct ttcttgtaca aagtggttga ttaattaagg ggaattctcg acctcgagac
     9721 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9781 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9841 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
     9901 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     9961 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
    10021 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
    10081 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
    10141 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
    10201 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
    10261 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
    10321 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
    10381 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatctgtg agtttgggga
    10441 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
    10501 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
    10561 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
    10621 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
    10681 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
    10741 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
    10801 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
    10861 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
    10921 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
    10981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
    11041 ttggcaaaga attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa
    11101 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
    11161 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
    11221 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
    11281 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
    11341 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
    11401 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
    11461 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
    11521 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
    11581 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
    11641 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
    11701 gtccgcgtac agcggctccc gggagttcta ggg
//
