LOCUS       sequence-171402-        9701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN283
FEATURES             Location/Qualifiers
     source          1..9701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(296..316)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(336..352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(336..352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(349..371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(498..1086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(578..597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1257..2048,2049..2117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1880..1899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2118..2222)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2290..2308
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2346..2368)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2681..2703
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2695..2712
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2696..2712
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2720..2740
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3787..3811
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8352..8363
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8512..8536)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagtactttg gacagattcc acgctctcct ggggttcctg gtggtccgac tggtcctggc
       61 tgtcctgggt ttccgtcggc tcctggcttg ccgtctggtc ctggtggtcc ctttggtcca
      121 ggttgtcctg aaagatgtgg tgattagata tggattgaaa aactatgaaa tgattacctg
      181 gggttccgtc gtttcctggt tgtccggatg gtcctggtgg tccgactggt cctggctctc
      241 cggtggatcc tggctctccc ttttggactg gctcgcagac agctggaagt ccctccaact
      301 tttctataca aagttgatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      361 tgttatccgc tggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa agaccaggaa ccgtaaaaag
      481 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      841 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      901 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      961 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1021 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1081 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1141 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1201 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1261 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1321 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tgaccccagt
     1381 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1441 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1501 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1561 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1621 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1681 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1741 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1801 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1861 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1921 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1981 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2041 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2101 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2221 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2281 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2341 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2401 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2461 aactatgcgg catcagagca gattgcactg agagtgcacc atatgcggtg tgaaataccg
     2521 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     2581 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2641 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2701 acgacggcca gtgaattatc aactatgtat aataaagttg ggcccattag gtcagacaaa
     2761 aatttctgat ctttaaacct tattgattaa aactaatttt tgcccgactt ttttgacaca
     2821 tgcactagac gcttgtactc aaaatttgga aaccttgctc aaatccacta ctcagatatg
     2881 acagtagcat cgataattga accattatcc tgaaatcccc tttcctgtcc cggttcattt
     2941 tccaattttt tcaacggtgg aagaaggggc aaattttata ccaaaacgtc tccgtttatg
     3001 acccaccccc tatgcaaaat aaacggctct gggagaaata gtacagaaaa cgcggagcct
     3061 gcttgattcc ttctccgagg cgacaaaaaa cgcatcattc gtattgtaag atgcatgtgt
     3121 ggctggcctc ttttgaattt tactggtttt ttatatttct actaatattt cgaaaaattt
     3181 tgtaagtaaa tcattgatta cgtttacaaa aatttagaaa catttcaaaa tgattacttc
     3241 taaattaatt atatttacag gttagttcga gttacttatg accttccaat ccgccatatc
     3301 cgccaaggac gctccaatta attttttaga attatttttt aacacatgta ttatttttta
     3361 acaataaatt attcatgaaa tcatcaaatg tatatgttct actataatat ttaggtcaaa
     3421 ctatagaaaa gaaatccttg tttttggaac aaaggagttc agatcctgtg catttgatct
     3481 tggatccaca tctttggtcc atgggaaagc ttggcactga tcaagaagga ttcttcaaat
     3541 atctaaaatt gccggctgcc cgtagaattc catcagtttt ccagagtttt agtgaaaatt
     3601 tgcatcccgt tagaaacaat ctacacaacg gtaaaaagga cccattattt ttaccgtacc
     3661 gttttccttt atggccctct atgcaaaaaa ttatagcgtg gaggggcagt acagaataat
     3721 aaagtgtgag aaaaaaaact gctctgcgag aaatagtaca gcaaacgcgg agcatgcttg
     3781 attctatcca ctttgtacaa gaaagctggg ttaatacgac tcactagtgg gcagatcttc
     3841 gaatgcatcg cgcgcaccgt acgtctcgag gagccggctg tagcatgcgg ccgcgctcta
     3901 ttgagcaact ttgggatcat atgtacacac caatgccctt ttcccaaatc ttttcctgtc
     3961 cttttctcta aaaacaataa atccatgcct attccagtat gacccctttg aagcagatat
     4021 aatcgcacaa acatatacac atagctcgga taaatgtaga aaaagaagaa aagaagtata
     4081 agtagataga tgctttccgg caattatcca ccgcaccgta gtcttcacca actgagactg
     4141 cgtcgttagg agacgccgac atgattcaga agcagaattt ggaagaaaaa cgacgatgat
     4201 attgaggctg gcacacatac accggaatat tcgacatgcc accacatcta gattccaagg
     4261 caatctctac ctcttcccat tctttcggtt tttttgttct gacaagaaaa gtggatagct
     4321 acgggctcaa tgagctgatt ttatttttaa atatcttaaa actatactag attcatgtgt
     4381 tttcaggtcc atattccaaa ttagtcgaaa agctgatccc gctacggttt actcgaatct
     4441 caacaatttt tagccggtac caaaaatgcc acaattcgac attctctgta agacaccacc
     4501 aaaagtcctc gtccgtcaat tcgtcgagcg tttcgagcgt ccatccggag agaagatcgc
     4561 cctctgcgcc gccgagctca cctacctctg ctggatgatc acccacaacg gaaccgccat
     4621 caagcgtgcc accttcatgt cctacaacac catcatctcc aactccctct ccttcgacat
     4681 cgtcaacaag tccctccaat tcaagtacaa gacccaaaag gccaccatcc tcgaggcctc
     4741 cctcaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagaa
     4801 gctcatccca gcctgggagt tcaccatcat cccatactac ggacaaaagc accaatccga
     4861 catcaccgac atcgtctcct ccctccaact ccaattcgag tcctccgagg aggccgacaa
     4921 gggaaactcc cactccaaga agatgctcaa ggccctcctc tccgagggag agtccatctg
     4981 ggagatcacc gagaagatcc tcaactcctt cgagtacacc tcccgtttca ccaagaccaa
     5041 gaccctctac caattcctct tcctcgccac cttcatcaac tgcggacgtt tctccgacat
     5101 caagaacgtc gacccaaagt ccttcaagct cgtccaaaac aagtacctcg gagtcatcat
     5161 ccaatgcctc gtcaccgaga ccaagacctc cgtctcccgt cacatctact tcttctccgc
     5221 ccgtggacgt atcgacccac tcgtctacct cgacgagttc ctccgtaact ccgagccagt
     5281 cctcaagcgt gtcaaccgta ccggaaactc ctcctccaac aagcaagagt accaactcct
     5341 caaggacaac ctcgtccgtt cctacaacaa ggccctcaag aagaacgccc catactccat
     5401 cttcgccatc aagaacggac caaagtccca catcggacgt cacctcatga cctccttcct
     5461 ctccatgaag gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     5521 gggactcacc gagctcacca acgtcgtcgg aaactggtcc gacaagcgtg cctccgccgt
     5581 cgcccgtacc acctacaccc accaaatcac cgccatccca gaccactact tcgccctcgt
     5641 ctcccgttac tacgcctacg acccaatctc caaggagatg atcgccctca aggacgagac
     5701 caacccaatc gaggagtggc aacacatcga gcaactcaag ggatccgccg agggatccat
     5761 ccgttaccca gcctggaacg gaatcatctc ccaagaggtc ctcgactacc tctcctccta
     5821 catcaaccgt cgtatctaac gcgtatgtat ctgcatatcc ttcaaatgtt gtccatattg
     5881 tatcagtaaa ttataatgcc ccctttttat atattctcct tatcatgtaa atgttctatt
     5941 gattttttgc tggtgtaaaa cgtttttata gtaaatcctc tccacagtga atttgttaaa
     6001 ttgtatcaaa acaagacatt gattaaattt gttcgagact tgtggattgt aaaatatttt
     6061 gcagaaaatg gcaatattca tttagaaaca ttacttccat aataggaaaa actatttatg
     6121 agttttcatg tgcctatgca agttcccact ctgtgtgaat ttcatcatac aaattgagca
     6181 aacataaggt ttatgtccag tatgggttct cagatgtttt gtaagagttg agctgtcggt
     6241 gaatgccttc tcacattgag cacatggata cggtctctct ccagtgtgtg ttctgaaata
     6301 atgaaaaata gtcttgcaat atgtttctac tattcgagaa aaaatgtaat tttttttctt
     6361 atggtaaccc ggctttcccc gggcctagtt ctagacattc tctaatgaaa aaatctttca
     6421 gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt ccgtgtgatt
     6481 agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg gggaactgaa
     6541 aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact agacaggaat
     6601 tattggaatt taatcacatt tggagttttt tttctattcg acagaataat tatccagaac
     6661 atttttgtat taaatattta tgcatcatat gagtagtcgg ctttgttgtg catgacgagt
     6721 ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt ttggattgtt gatcatgtcg
     6781 tccactgaaa aagagattag tcttttgaat tgtacttttt agaataatga ctcactgagt
     6841 tgttgagaga gttgagggaa ctcatagata tgttcacagt tgtttcgtga attcggaata
     6901 cagaatccga attcaaagtc aaaacacttc agaaggcgat ctttgaagaa gtgacgttcg
     6961 atcattcgga aatgatggat tgggatgtct cctactttga attccacagt tgctccgacc
     7021 gttttgagtt tcaaaaagtt tggagcaaat ctataacgca cgtatcttgc cgactcctgt
     7081 ggcgactcat cattctcctg atcgttctcc ggtttggcga tctcgaaaag cacttgctca
     7141 gtgtccagat cacggatttg gaacttggtg aactcgatgt tatagatgtt cgcagatggg
     7201 gagcataaga atcctaaatt tatgttttaa actgaaatcc aaagggagca agataccttg
     7261 agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt tgagctttct tcgcaagctc
     7321 cgattccgtt gtgattcctt gttcggtgct tggtggtggc cgtggcatct ggaaatatgg
     7381 aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag ttagttgagc
     7441 ggtttctcta gttttctgag tctcacctgc gacgggaagg tcgccgagcc gggtggaatc
     7501 gatcgttgtt gctcggcttt catatcggtt tggttggaag cggctgaaaa cggaaagaag
     7561 tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt gccaaataac
     7621 cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga taatgatatc
     7681 agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac atgtaatcaa
     7741 aatacacttt gtaaattatc atttttattg aaactccacc atttttctat ttataacgct
     7801 aataatttga aaaagaaacc tattgcgaac cgcggggtga atcccaaaaa cgaatgcgtt
     7861 ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga atagagagct
     7921 cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg agggtcttct
     7981 tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg agaagggaaa
     8041 aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag cggaaaagca
     8101 gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc caagaaagga
     8161 atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca gaataacaga
     8221 tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta atttgggtca
     8281 atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg tttgttatca
     8341 gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt ttctgtcaaa
     8401 tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac attttatccg
     8461 ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa gagcctgctt
     8521 ttttgtacaa acttgaattc aaaaacgcag aactcgtact gttggatgcc tgtgtagcgg
     8581 tcctctattg atttttgcta ttttttgata attttactgt aaattatgag ttattccaat
     8641 taacattcct agctaaatgt aagttagcga ccaatttttt agcaacccca ttttatgact
     8701 tttcagaata tcgcctactg ggtcaacgac acttgtttgg aactaagggt cttcccaatt
     8761 ttttttaaat cttcataggc ttgacagaat cagggttctt caagcagctt tgagagggaa
     8821 tcaattgcaa catttgatgt taaatttata ttttttatag ttctctggtg aaatggggac
     8881 caccgcaaag ccccttttat gtttttaact tgaataaaat cttgtattaa attgtgattt
     8941 atagatctgt atgggtaatt tgaaggaaaa gaaagggagc tcccaaaagt gccttacaga
     9001 tccaggccca ataaatgtaa actattgggc agtagtatgg caaaaatcgt gcggaatttg
     9061 aaaaacctag aattaaaata aatattcaac acaaatgttc gagatatttt aagttcggag
     9121 tgtatatcta gaacatattg tacaagtagg tctacgggat cgtctacggg aaaatttgat
     9181 ttcctcgaag atgtatttaa tgaattctct aatgttgcag gtctttgtat ttgacagaat
     9241 gttggttttt gaaacttatt cgccataatt gcttggctac tacatgtctc atgccattga
     9301 cactatcctg tactcgaaat ttgttaagta actacccacc gtccacttat gacgagaggg
     9361 tcaattaagg tcactccaca aaagtaaatt tgaaaacaca tgagaaaaag tgatctgaat
     9421 tcccttgaag tggctaaaac aaagcagatg agggatacga cgtatgcgac tactgagaga
     9481 taaggtcgaa acttgataag gatgctgctt acctgcttag caagtttctt cgcacaggag
     9541 aaagtgacaa gctaaactat caaattcatc taaaacctca aaaaattatt ttggatgaaa
     9601 taatgtttta ttttgagcaa cgcataaggc aaagaataaa ttaaaggcga tccgcttagc
     9661 ggcgagttcc gtcctcgaag aagattcctc catcgatggc g
//
