LOCUS       sequence-171403-        9486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN266
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..57
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     54..70
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    90..110
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1571..1595
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1744..1755)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6081..6105)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7152..7172)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7180..7197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7180..7196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7189..7211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7524..7546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7584..7602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7670..7774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7775..7843,7844..8635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7993..8012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8806..9394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9295..9314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaccgtatt accgcctttg agtgagctga taccagcgga taacaatttc acacaggaaa
       61 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg gagggacttc
      121 cagctgtctg cgagccagtc caaaagggag agccaggatc caccggagag ccaggaccag
      181 tcggaccacc aggaccatcc ggacaaccag gaaacgacgg aaccccaggt aatcatttca
      241 tagtttttca atccatatct aatcaccaca tctttcagga caacctggac caaagggacc
      301 accaggacca gacggcaagc caggagccga cggaaaccca ggacagccag gaccagtcgg
      361 accaccagga accccaggag agcgtggaat ctgtccaaag tactgcgcca tcgatggagg
      421 aatcttcttc gaggacggaa ctcgccgcta agcggatcgc ctttaattta ttctttgcct
      481 tatgcgttgc tcaaaataaa acattatttc atccaaaata attttttgag gttttagatg
      541 aatttgatag tttagcttgt cactttctcc tgtgcgaaga aacttgctaa gcaggtaagc
      601 agcatcctta tcaagtttcg accttatctc tcagtagtcg catacgtcgt atccctcatc
      661 tgctttgttt tagccacttc aagggaattc agatcacttt ttctcatgtg ttttcaaatt
      721 tacttttgtg gagtgacctt aattgaccct ctcgtcataa gtggacggtg ggtagttact
      781 taacaaattt cgagtacagg atagtgtcaa tggcatgaga catgtagtag ccaagcaatt
      841 atggcgaata agtttcaaaa accaacattc tgtcaaatac aaagacctgc aacattagag
      901 aattcattaa atacatcttc gaggaaatca aattttcccg tagacgatcc cgtagaccta
      961 cttgtacaat atgttctaga tatacactcc gaacttaaaa tatctcgaac atttgtgttg
     1021 aatatttatt ttaattctag gtttttcaaa ttccgcacga tttttgccat actactgccc
     1081 aatagtttac atttattggg cctggatctg taaggcactt ttgggagctc cctttctttt
     1141 ccttcaaatt acccatacag atctataaat cacaatttaa tacaagattt tattcaagtt
     1201 aaaaacataa aaggggcttt gcggtggtcc ccatttcacc agagaactat aaaaaatata
     1261 aatttaacat caaatgttgc aattgattcc ctctcaaagc tgcttgaaga accctgattc
     1321 tgtcaagcct atgaagattt aaaaaaaatt gggaagaccc ttagttccaa acaagtgtcg
     1381 ttgacccagt aggcgatatt ctgaaaagtc ataaaatggg gttgctaaaa aattggtcgc
     1441 taacttacat ttagctagga atgttaattg gaataactca taatttacag taaaattatc
     1501 aaaaaatagc aaaaatcaat agaggaccgc tacacaggca tccaacagta cgagttctgc
     1561 gtttttgaat tcaagtttgt acaaaaaagc aggctctttg agccaattta tccaagtcct
     1621 tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat caaaaacaat
     1681 ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg gctcaaagtt
     1741 agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc ccaacaaggg
     1801 atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca aattcatcgc
     1861 ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt ctgcacccta
     1921 tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt ctccccagct
     1981 ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct ctctgacgtc
     2041 ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt ttcccatttc
     2101 tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt ttttttctgt
     2161 cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc tttttcttct
     2221 tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac cccgcggttc
     2281 gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg gagtttcaat
     2341 aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt tgaaattttt
     2401 cataccagtt ttccggaaat ccatctgata tcattatcgt atttttcttt ttaaaaatgt
     2461 tttcaaaaaa aaacaaaata tagctggtta tttggcaccc tctaattacc attttcctgt
     2521 cacaccacac tcttttcctt cttccacttc tttccgtttt cagccgcttc caaccaaacc
     2581 gatatgaaag ccgagcaaca acgatcgatt ccacccggct cggcgacctt cccgtcgcag
     2641 gtgagactca gaaaactaga gaaaccgctc aactaactct tgatatccga tttcattctt
     2701 ttctcttttc tttttgttga acttttccat atttccagat gccacggcca ccaccaagca
     2761 ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa agctcaaatc actccgaacg
     2821 acgttttagc acttccggga atcactcaag gtatcttgct ccctttggat ttcagtttaa
     2881 aacataaatt taggattctt atgctcccca tctgcgaaca tctataacat cgagttcacc
     2941 aagttccaaa tccgtgatct ggacactgag caagtgcttt tcgagatcgc caaaccggag
     3001 aacgatcagg agaatgatga gtcgccacag gagtcggcaa gatacgtgcg ttatagattt
     3061 gctccaaact ttttgaaact caaaacggtc ggagcaactg tggaattcaa agtaggagac
     3121 atcccaatcc atcatttccg aatgatcgaa cgtcacttct tcaaagatcg ccttctgaag
     3181 tgttttgact ttgaattcgg attctgtatt ccgaattcac gaaacaactg tgaacatatc
     3241 tatgagttcc ctcaactctc tcaacaactc agtgagtcat tattctaaaa agtacaattc
     3301 aaaagactaa tctctttttc agtggacgac atgatcaaca atccaaacga gactcgttct
     3361 gacagcttct atttcgtcga taacaaactc gtcatgcaca acaaagccga ctactcatat
     3421 gatgcataaa tatttaatac aaaaatgttc tggataatta ttctgtcgaa tagaaaaaaa
     3481 actccaaatg tgattaaatt ccaataattc ctgtctagtt tgttccttcc ccttcccctt
     3541 ctcatgttca atgcattcct aagcttttca gttcccccct tgtttctata ttttttcgct
     3601 gtcctgtcac actcgctaaa aacactaatc acacggaaat ctgttttcaa taaaaactcc
     3661 aactttaact cattttcaat ttcaactgaa agattttttc attagagaat gtctagaact
     3721 aggcccgggg aaagccgggt taccataaga aaaaaaatta cattttttct cgaatagtag
     3781 aaacatattg caagactatt tttcattatt tcagaacaca cactggagag agaccgtatc
     3841 catgtgctca atgtgagaag gcattcaccg acagctcaac tcttacaaaa catctgagaa
     3901 cccatactgg acataaacct tatgtttgct caatttgtat gatgaaattc acacagagtg
     3961 ggaacttgca taggcacatg aaaactcata aatagttttt cctattatgg aagtaatgtt
     4021 tctaaatgaa tattgccatt ttctgcaaaa tattttacaa tccacaagtc tcgaacaaat
     4081 ttaatcaatg tcttgttttg atacaattta acaaattcac tgtggagagg atttactata
     4141 aaaacgtttt acaccagcaa aaaatcaata gaacatttac atgataagga gaatatataa
     4201 aaagggggca ttataattta ctgatacaat atggacaaca tttgaaggat atgcagatac
     4261 atacgcgtta gatacgacgg ttgatgtagg aggagaggta gtcgaggacc tcttgggaga
     4321 tgattccgtt ccaggctggg taacggatgg atccctcggc ggatcccttg agttgctcga
     4381 tgtgttgcca ctcctcgatt gggttggtct cgtccttgag ggcgatcatc tccttggaga
     4441 ttgggtcgta ggcgtagtaa cgggagacga gggcgaagta gtggtctggg atggcggtga
     4501 tttggtgggt gtaggtggta cgggcgacgg cggaggcacg cttgtcggac cagtttccga
     4561 cgacgttggt gagctcggtg agtccctgaa aatttaaatc agattagtta gttagtaaaa
     4621 tcatgtttaa acttaccttc atggagagga aggaggtcat gaggtgacgt ccgatgtggg
     4681 actttggtcc gttcttgatg gcgaagatgg agtatggggc gttcttcttg agggccttgt
     4741 tgtaggaacg gacgaggttg tccttgagga gttggtactc ttgcttgttg gaggaggagt
     4801 ttccggtacg gttgacacgc ttgaggactg gctcggagtt acggaggaac tcgtcgaggt
     4861 agacgagtgg gtcgatacgt ccacgggcgg agaagaagta gatgtgacgg gagacggagg
     4921 tcttggtctc ggtgacgagg cattggatga tgactccgag gtacttgttt tggacgagct
     4981 tgaaggactt tgggtcgacg ttcttgatgt cggagaaacg tccgcagttg atgaaggtgg
     5041 cgaggaagag gaattggtag agggtcttgg tcttggtgaa acgggaggtg tactcgaagg
     5101 agttgaggat cttctcggtg atctcccaga tggactctcc ctcggagagg agggccttga
     5161 gcatcttctt ggagtgggag tttcccttgt cggcctcctc ggaggactcg aattggagtt
     5221 ggagggagga gacgatgtcg gtgatgtcgg attggtgctt ttgtccgtag tatgggatga
     5281 tggtgaactc ccaggctggg atgagcttct gaaaatttaa ataatcaggg ttagttagta
     5341 tatatatgtt taaacttacc ttgagggagg cctcgaggat ggtggccttt tgggtcttgt
     5401 acttgaattg gagggacttg ttgacgatgt cgaaggagag ggagttggag atgatggtgt
     5461 tgtaggacat gaaggtggca cgcttgatgg cggttccgtt gtgggtgatc atccagcaga
     5521 ggtaggtgag ctcggcggcg cagagggcga tcttctctcc ggatggacgc tcgaaacgct
     5581 cgacgaattg acggacgagg acttttggtg gtgtcttaca gagaatgtcg aattgtggca
     5641 tttttggtac cttatctgga acaaaatgta agaatattct taaaaattgt ttcattcaga
     5701 atgaatcttg ttttgttttc acagagcggt agaaaaaata tttcgagttt tgacaaaaaa
     5761 tataaattaa aaaaaagaac agggtgtgat aaatgaattg aattgaaaag aacatggatt
     5821 gtagaaaacg gaaaggtgga tgaaggtgga gtgtatctgt taaataggtc agtctttatc
     5881 gacacaataa cccaattatc ggagataaga aaagattcca aacgagaaga gatgagtaca
     5941 tgcgaaaaag gaatgtagaa tataagggat gtgttgcgag aaatcgtgga atgagatgcg
     6001 gccgcatgct acagccggct cctcgagacg tacggtgcgc gcgatgcatt cgaagatctg
     6061 cccactagtg agtcgtatta acccagcttt cttgtacaaa gtggatagaa tcaagcatgc
     6121 tccgcgtttg ctgtactatt tctcgcagag cagttttttt tctcacactt tattattctg
     6181 tactgcccct ccacgctata attttttgca tagagggcca taaaggaaaa cggtacggta
     6241 aaaataatgg gtccttttta ccgttgtgta gattgtttct aacgggatgc aaattttcac
     6301 taaaactctg gaaaactgat ggaattctac gggcagccgg caattttaga tatttgaaga
     6361 atccttcttg atcagtgcca agctttccca tggaccaaag atgtggatcc aagatcaaat
     6421 gcacaggatc tgaactcctt tgttccaaaa acaaggattt cttttctata gtttgaccta
     6481 aatattatag tagaacatat acatttgatg atttcatgaa taatttattg ttaaaaaata
     6541 atacatgtgt taaaaaataa ttctaaaaaa ttaattggag cgtccttggc ggatatggcg
     6601 gattggaagg tcataagtaa ctcgaactaa cctgtaaata taattaattt agaagtaatc
     6661 attttgaaat gtttctaaat ttttgtaaac gtaatcaatg atttacttac aaaatttttc
     6721 gaaatattag tagaaatata aaaaaccagt aaaattcaaa agaggccagc cacacatgca
     6781 tcttacaata cgaatgatgc gttttttgtc gcctcggaga aggaatcaag caggctccgc
     6841 gttttctgta ctatttctcc cagagccgtt tattttgcat agggggtggg tcataaacgg
     6901 agacgttttg gtataaaatt tgccccttct tccaccgttg aaaaaattgg aaaatgaacc
     6961 gggacaggaa aggggatttc aggataatgg ttcaattatc gatgctactg tcatatctga
     7021 gtagtggatt tgagcaaggt ttccaaattt tgagtacaag cgtctagtgc atgtgtcaaa
     7081 aaagtcgggc aaaaattagt tttaatcaat aaggtttaaa gatcagaaat ttttgtctga
     7141 cctaatgggc ccaactttat tatacatagt tgataattca ctggccgtcg ttttacaacg
     7201 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7261 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7321 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7381 acaccgcata tggtgcactc tcagtgcaat ctgctctgat gccgcatagt taagccagcc
     7441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggtc
     8521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9421 gttcctggtc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9481 tgtgga
//
