LOCUS       sequence-171406-       13482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN282
FEATURES             Location/Qualifiers
     source          1..13482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..44
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     41..57
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     41..57
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    77..97
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1558..1582
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1731..1742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(6930..6941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10064..10088)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11135..11155)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11163..11180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11163..11179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11172..11194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11507..11529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11567..11585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11653..11757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11758..11826,11827..12618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11976..11995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12789..13377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13278..13297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cagcggataa caatttcaca caggaaacag ctatgaccat
       61 gattacgcca agctatcaac tttgtataga aaagttggag ggacttccag ctgtctgcga
      121 gccagtccaa aagggagagc caggatccac cggagagcca ggaccagtcg gaccaccagg
      181 accatccgga caaccaggaa acgacggaac cccaggtaat catttcatag tttttcaatc
      241 catatctaat caccacatct ttcaggacaa cctggaccaa agggaccacc aggaccagac
      301 ggcaagccag gagccgacgg aaacccagga cagccaggac cagtcggacc accaggaacc
      361 ccaggagagc gtggaatctg tccaaagtac tgcgccatcg atggaggaat cttcttcgag
      421 gacggaactc gccgctaagc ggatcgcctt taatttattc tttgccttat gcgttgctca
      481 aaataaaaca ttatttcatc caaaataatt ttttgaggtt ttagatgaat ttgatagttt
      541 agcttgtcac tttctcctgt gcgaagaaac ttgctaagca ggtaagcagc atccttatca
      601 agtttcgacc ttatctctca gtagtcgcat acgtcgtatc cctcatctgc tttgttttag
      661 ccacttcaag ggaattcaga tcactttttc tcatgtgttt tcaaatttac ttttgtggag
      721 tgaccttaat tgaccctctc gtcataagtg gacggtgggt agttacttaa caaatttcga
      781 gtacaggata gtgtcaatgg catgagacat gtagtagcca agcaattatg gcgaataagt
      841 ttcaaaaacc aacattctgt caaatacaaa gacctgcaac attagagaat tcattaaata
      901 catcttcgag gaaatcaaat tttcccgtag acgatcccgt agacctactt gtacaatatg
      961 ttctagatat acactccgaa cttaaaatat ctcgaacatt tgtgttgaat atttatttta
     1021 attctaggtt tttcaaattc cgcacgattt ttgccatact actgcccaat agtttacatt
     1081 tattgggcct ggatctgtaa ggcacttttg ggagctccct ttcttttcct tcaaattacc
     1141 catacagatc tataaatcac aatttaatac aagattttat tcaagttaaa aacataaaag
     1201 gggctttgcg gtggtcccca tttcaccaga gaactataaa aaatataaat ttaacatcaa
     1261 atgttgcaat tgattccctc tcaaagctgc ttgaagaacc ctgattctgt caagcctatg
     1321 aagatttaaa aaaaattggg aagaccctta gttccaaaca agtgtcgttg acccagtagg
     1381 cgatattctg aaaagtcata aaatggggtt gctaaaaaat tggtcgctaa cttacattta
     1441 gctaggaatg ttaattggaa taactcataa tttacagtaa aattatcaaa aaatagcaaa
     1501 aatcaataga ggaccgctac acaggcatcc aacagtacga gttctgcgtt tttgaattca
     1561 agtttgtaca aaaaagcagg ctctttgagc caatttatcc aagtccttgt aaaaagtatt
     1621 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1681 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1741 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1801 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1861 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1921 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1981 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     2041 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     2101 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     2161 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     2221 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2281 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2341 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2401 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2461 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2521 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2581 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2641 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2701 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2761 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2821 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2881 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2941 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     3001 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     3061 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     3121 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     3181 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3241 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3301 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3361 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3421 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3481 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3541 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3601 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3661 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccggggaaa
     3721 gccgggttac cataagaaaa aaaattacat tttttctcga atagtagaaa catattgcaa
     3781 gactattttt cattatttca gaacacacac tggagagaga ccgtatccat gtgctcaatg
     3841 tgagaaggca ttcaccgaca gctcaactct tacaaaacat ctgagaaccc atactggaca
     3901 taaaccttat gtttgctcaa tttgtatgat gaaattcaca cagagtggga acttgcatag
     3961 gcacatgaaa actcataaat agtttttcct attatggaag taatgtttct aaatgaatat
     4021 tgccattttc tgcaaaatat tttacaatcc acaagtctcg aacaaattta atcaatgtct
     4081 tgttttgata caatttaaca aattcactgt ggagaggatt tactataaaa acgttttaca
     4141 ccagcaaaaa atcaatagaa catttacatg ataaggagaa tatataaaaa gggggcatta
     4201 taatttactg atacaatatg gacaacattt gaaggatatg cagatacata cgcgttagat
     4261 acgacggttg atgtaggagg agaggtagtc gaggacctct tgggagatga ttccgttcca
     4321 ggctgggtaa cggatggatc cctcggcgga tcccttgagt tgctcgatgt gttgccactc
     4381 ctcgattggg ttggtctcgt ccttgagggc gatcatctcc ttggagattg ggtcgtaggc
     4441 gtagtaacgg gagacgaggg cgaagtagtg gtctgggatg gcggtgattt ggtgggtgta
     4501 ggtggtacgg gcgacggcgg aggcacgctt gtcggaccag tttccgacga cgttggtgag
     4561 ctcggtgagt ccctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact
     4621 taccttcatg gagaggaagg aggtcatgag gtgacgtccg atgtgggact ttggtccgtt
     4681 cttgatggcg aagatggagt atggggcgtt cttcttgagg gccttgttgt aggaacggac
     4741 gaggttgtcc ttgaggagtt ggtactcttg cttgttggag gaggagtttc cggtacggtt
     4801 gacacgcttg aggactggct cggagttacg gaggaactcg tcgaggtaga cgagtgggtc
     4861 gatacgtcca cgggcggaga agaagtagat gtgacgggag acggaggtct tggtctcggt
     4921 gacgaggcat tggatgatga ctccgaggta cttgttttgg acgagcttga aggactttgg
     4981 gtcgacgttc ttgatgtcgg agaaacgtcc gcagttgatg aaggtggcga ggaagaggaa
     5041 ttggtagagg gtcttggtct tggtgaaacg ggaggtgtac tcgaaggagt tgaggatctt
     5101 ctcggtgatc tcccagatgg actctccctc ggagaggagg gccttgagca tcttcttgga
     5161 gtgggagttt cccttgtcgg cctcctcgga ggactcgaat tggagttgga gggaggagac
     5221 gatgtcggtg atgtcggatt ggtgcttttg tccgtagtat gggatgatgg tgaactccca
     5281 ggctgggatg agcttctgaa aatttaaata atcagggtta gttagtatat atatgtttaa
     5341 acttaccttg agggaggcct cgaggatggt ggccttttgg gtcttgtact tgaattggag
     5401 ggacttgttg acgatgtcga aggagaggga gttggagatg atggtgttgt aggacatgaa
     5461 ggtggcacgc ttgatggcgg ttccgttgtg ggtgatcatc cagcagaggt aggtgagctc
     5521 ggcggcgcag agggcgatct tctctccgga tggacgctcg aaacgctcga cgaattgacg
     5581 gacgaggact tttggtggtg tcttacagag aaggtcgaat tgtggcattt ttggtacctc
     5641 tataatctat tttctagttt ctattttatt agaatgccaa gaaaatcaag catttataca
     5701 tcaaaactta tcaatgaaaa cagtggcacc taaaacatca agcagtttaa aaattcaaaa
     5761 attcaaaaat gcttcgagaa gtgtattttc ttaaatactg cctttaaaat taaaactatt
     5821 tgaagacaat tttcagacga agatattaca attaacattt tcttgctcat tccaaaaatt
     5881 aactgcaaaa attaattaaa ataacccata aaataagaac acaaagatgt taatgccata
     5941 gtaacttaga agatatcagt gaaaacagtg gcacctcgat agctgttcaa cgataagact
     6001 aaaaatgatt gggaagtata gataggctga ttttatcaaa aagaaaattg tcaatgaagg
     6061 cacaaagcgt attggtttga aaagaaagat ggtgtgcaca ccgagctcgg tgtgcatgat
     6121 ggctggctag cgattatggc gttagtgtcg actattctta tcagtttcag ccccgacctc
     6181 tgtcgctcat atacgcagac aaccacccac ttccagccaa acatgggact cgggtccgcc
     6241 catcattgca tgtacggtag ttggtaattg aaaaaagtgg aatgaggaag aaaatattgg
     6301 tttcaaagta gcgtttataa taactacaaa aaaatcaact gaggagacaa taagtagata
     6361 aaacaggcga tcagactttt gatggataca tatatccaat tttgcaaaca aacttgaaaa
     6421 ttaaattgag tttttgcgtt ttcactgaaa ctttcaaaca agttgcaatg cagctattaa
     6481 tactgcacat agtaatagtc ttgctttaat ttattgccag aacagatgta tctagttaaa
     6541 atgttgataa attactatcc ttcacagagt gaaacaaaat caaaagcttt caagaattta
     6601 tggtaatttt ttttaatttg ataaggcatc attgtcaaaa cttgaataat ttgtcaaaag
     6661 ctagatattc aaaaattagt attgaacctc aattacccta caaagtttat agtgctcacc
     6721 aattggaaat aagttttgaa tacaatacta atggagaaaa tacggcaaac tatacatttt
     6781 taacttttca ctcattagtt ttcatataat ttttaaactg tgatgtcaca ccataatttt
     6841 aaagacaaga aaacatacaa atacatataa ttatcaataa atgatattat tataaaataa
     6901 aaaacgcata tttgtgagat atgaaaagta cgcccttcta tgtttttttt caagaatacc
     6961 ggatcaaagg gtacgctttt tgttacacag tgtgttttac ggaaacatat tcaacatttg
     7021 gaggacagtt ctgtctccaa agctgaccag ttcaacattc ttataaacaa aacgtgatgc
     7081 gaccttcaag ttgtctggaa cttttgtgat tcgggcagga taaggagtat gaagaataca
     7141 ataatgaaaa ataaaacaaa aaaagataac ctgtagaaaa attgataatt ttcagttttt
     7201 ttcgataatc agccacacac gcacataata ttacttcaca ccccacaata tcagataata
     7261 atcaaaaatg ataaaatata tctttataca tcgataacac aatcaaacta gtgataaatt
     7321 ggtatttcaa gatttcaacc atatgctctc cgaactgtat actacagtaa tattattggg
     7381 tacaaggtct aaaaacatta gcatgttgta aaaccacttc ttttgctctc atttctcaat
     7441 gtttgcgctc agtaagtgag cttcttttga aatgaagtac ggtcgcctta tcagttgcat
     7501 gacgtttgag ccgattcttt cctttttgtc ttcaaatttc ctattcagcc agcaaactcg
     7561 gggaagtcaa ggatgaacgt ttaatagaga aaaactatgg gtgttctgaa acaatcagta
     7621 aaaaaattat caggacaaaa tggcaacatt atcgttgaga atgcgtcctc gtatcgccgt
     7681 agctcagaag tagaaatcaa ttgaagataa cattatcaat tctgtataaa aagaaaatac
     7741 aggttttgat atcaaacctt aacacattta acatttggtc attatattcc ataatagtgt
     7801 catcaattta gttctgcaaa aacacatctg ctcacactac tctctatatc cgaaatgaca
     7861 caattagctt tcgaagttat ttatttggtg tcgatagttg tatatgagtg ttgtgactcc
     7921 gtttataaac cgtttttaac agtcgggaat aaatttatat ttttcacttt aaaattaatc
     7981 tggtatcttt tttttaattt ggttttttaa cggggggttt gaagttcaaa gaaaatcaaa
     8041 atcaaaattc tttatttaga aagtttgtta agtaattgaa ggttttttgg agattctaaa
     8101 atcagctgta taagtatttc taaacagcgt atctttcaaa aaagaaaaac ttttttgacg
     8161 tcaaagggtt agaaaatcac tttcacataa tttctggatg tcaaacggtc cgagagcttt
     8221 tttaatacgt gaaacattcc atcacgtctt cattttagtt ttttttttta tttatataat
     8281 tcaatttatg tttcttaaaa ctgtaaattt atatttatta agtcagtaca aaaaattctg
     8341 aattggtcaa ttaggaactg tgaacgtttc agagtagatt tgtgatgacg gttctttttt
     8401 gtttattaaa catttcattt atgaatatca cattttccca tcagttcttt tttttttcaa
     8461 attcatgatc acaggaaaca ttttataact gtcagaatac ttggtgcgag ccatcacacg
     8521 aaggcatgca aaaacttgat tgaacattga tttttttgta ctttcaggga tattcaggta
     8581 aaaactgaac attggttagc ggtgatttct tctggtgccg gtaaagatgt atataacttt
     8641 atttgtgttc aaaaataacc atggaagtat gttagcaagt acatttttaa cgttttcaat
     8701 ctgattattt ttcaattagt atgactcatc gagcattttt agtttttttt tcagttttag
     8761 aatcagaaat ctacaaaaaa agcactctac tcaagatcag tcattttctg atgatttatt
     8821 gttttctcgc cactaatttc ctactttgat catcattttt tatcattaac atttttgaat
     8881 ttatattatt agattataac ttgatgaatg tttgcaactg tattcttaat gctcctgttt
     8941 tttcgtccca cataagtctc taaatactat ttctaaacat atttgatact ttccaagcat
     9001 ttattgggtc tttcacattc gatctgttat cagtagttat gcacacgaca agacactaga
     9061 tggtacaatc gagaggcaat aaactgttta cacattcttt tcagttcgtc ttattctctc
     9121 tcactttctc attgtcttta ttagttgaga aaatgtacgg gttgggggag aactaagagc
     9181 agaatgggaa aaaaggtggg taataggatt ataataataa tagttacaga gtcaggaatt
     9241 ggttatcaag aattggagtt catacatttc gaaattagag atgctgaacg ggttcgatgg
     9301 cgtgcattgg agtagaagaa tctgaagatg gagtcattgg taaagtgaca ggtgatgcca
     9361 aagatgttgt tgacgaaatg gttgaatctg ctgaaatttg tcgattaatc aattcgttgt
     9421 aatttttcaa acgtcatgat gactcattca acttatcaca tacctccgtg gctgccggaa
     9481 gttgtggttt gcagcacatg ggaaggagaa gatcccacct gcaattcaaa cttttgttgc
     9541 ggacaatgtg tatattacct accgaagatt gcgtagcatg atccagcgga gagtcccctg
     9601 atgcgtcttt ctcatccgta tttgcattgc caaaaatatt atcagtgacc gttgggtttc
     9661 tgtaatagta gtaattcatc tttcggcaat tcctatcata ttgtaatgat tacctaacat
     9721 agaacattgc gaccaacgtt gagacgacca ctaaaaccac gattcctata ataggaattg
     9781 caactttagg ggaagttaaa gttttaatgt ctaccatttt taagttctca gttgttagga
     9841 atgcacaaga gaattgttga caggtggcag tataaaattg aaaaatgagc tcctggcccg
     9901 tcttgaagcc gcattttacg atgattgtga cgcacattaa atcttctttg gcacttctaa
     9961 accgatgtat atccgtctca gcggccgcat gctacagccg gctcctcgag acgtacggtg
    10021 cgcgcgatgc attcgaagat ctgcccacta gtgagtcgta ttaacccagc tttcttgtac
    10081 aaagtggata gaatcaagca tgctccgcgt ttgctgtact atttctcgca gagcagtttt
    10141 ttttctcaca ctttattatt ctgtactgcc cctccacgct ataatttttt gcatagaggg
    10201 ccataaagga aaacggtacg gtaaaaataa tgggtccttt ttaccgttgt gtagattgtt
    10261 tctaacggga tgcaaatttt cactaaaact ctggaaaact gatggaattc tacgggcagc
    10321 cggcaatttt agatatttga agaatccttc ttgatcagtg ccaagctttc ccatggacca
    10381 aagatgtgga tccaagatca aatgcacagg atctgaactc ctttgttcca aaaacaagga
    10441 tttcttttct atagtttgac ctaaatatta tagtagaaca tatacatttg atgatttcat
    10501 gaataattta ttgttaaaaa ataatacatg tgttaaaaaa taattctaaa aaattaattg
    10561 gagcgtcctt ggcggatatg gcggattgga aggtcataag taactcgaac taacctgtaa
    10621 atataattaa tttagaagta atcattttga aatgtttcta aatttttgta aacgtaatca
    10681 atgatttact tacaaaattt ttcgaaatat tagtagaaat ataaaaaacc agtaaaattc
    10741 aaaagaggcc agccacacat gcatcttaca atacgaatga tgcgtttttt gtcgcctcgg
    10801 agaaggaatc aagcaggctc cgcgttttct gtactatttc tcccagagcc gtttattttg
    10861 catagggggt gggtcataaa cggagacgtt ttggtataaa atttgcccct tcttccaccg
    10921 ttgaaaaaat tggaaaatga accgggacag gaaaggggat ttcaggataa tggttcaatt
    10981 atcgatgcta ctgtcatatc tgagtagtgg atttgagcaa ggtttccaaa ttttgagtac
    11041 aagcgtctag tgcatgtgtc aaaaaagtcg ggcaaaaatt agttttaatc aataaggttt
    11101 aaagatcaga aatttttgtc tgacctaatg ggcccaactt tattatacat agttgataat
    11161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    11221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    11281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
    11341 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtgc aatctgctct
    11401 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
    11461 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
    11521 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
    11581 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    11641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    11701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    11761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    11821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    11881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    11941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    12001 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    12061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    12121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    12181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    12241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    12301 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    12361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    12421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    12481 ggtatcattg cagcactggg gtcagatggt aagccctccc gtatcgtagt tatctacacg
    12541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    12601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    12661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    12721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    12781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    12841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    12901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    12961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    13021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    13081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    13141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    13201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    13261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    13321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    13381 gccagcaacg cggccttttt acggttcctg gtcttttgct ggccttttgc tcacatgttc
    13441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta cc
//
