LOCUS       sequence-171409-       10467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DamID control plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Gonzalez-Aguilera C, Ikegami K, Ayuso C, de Luis A, Iniguez M, 
            Cabello J, Lieb JD, Askjaer P
  TITLE     Genome-wide analysis links emerin to neuromuscular junction activity
            in Caenorhabditis elegans.
  JOURNAL   Genome Biol. 2014 Feb 3;15(2):R21. doi: 10.1186/gb-2014-15-2-r21.
  PUBMED    24490688
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2014-02-3"; volume: "15(2):R21. doi"; pages: " 
            10.1186/gb-2014-15-2-r21"
COMMENT     Sequence Label: pBN67
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             532..543
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2259..2278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2938..3729,3730..3798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3561..3580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3799..3903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3971..3989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4027..4049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7470..7499)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7558..7578)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8326..8354
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8407..8423
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8408..8424
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tattgcgaac cgcggggtga atcccaaaaa cgaatgcgtt ttggtggagt gattgattcg
       61 aatcgaagaa gaaaaagaag aagacgtgga atagagagct cactcttaac cgagcagcac
      121 acaccgacag aaaaaaaaat gaaatgaatg agggtcttct tcttcttctt cttcgaatga
      181 ttgacagaaa tgggaaaaag aggaagattg agaagggaaa aaggaaggag aaaagaagca
      241 gaagaagacg tcagagagga gaggaacgag cggaaaagca gcgggcgcaa gtcatagaag
      301 tagcagagct ggggagaaga agacactatc caagaaagga atgacgagag agtatgcaaa
      361 ggggtatagg gtgcagacag aataggaaca gaataacaga tgatgagcca agaagagttg
      421 aaaagggcga tgaatttgtc atgtaactta atttgggtca atttgagcat gatgaattga
      481 aatcatccct tgttgggagt taataaccgg tttgttatca gaaaccctgt aatagaaggg
      541 cgccctaact ttgagccaat tcatcccggt ttctgtcaaa tatatcaaaa agtggtcaac
      601 tgacaaattg tttttgatat tataataaac attttatccg ttaacaattt tcgaatactt
      661 tttacaagga cttggataaa ttggctcaaa gacgaagtga gtttgctacc atcatggaat
      721 caggaggaca tttcgacaat gtcatataat gtttgaaaaa tgattttaac aagggtctca
      781 ttcgagataa gcaatgctct gagcaagtgg ctcgtcgtct tcaagaatct cttgttgata
      841 ttcatttgga tcctgttttg cacgaagcat gtgccatgga tattcagagt ttttgcaaaa
      901 aagttccgcc aggtcaaaca atctttttaa tttttatatg atacttcggg tgtatttttc
      961 ttctttttcc tgttcatttt cgcttttttc tcactttttt gtaacaaaat ctctcacttt
     1021 tctgtaagtc ttaacaaaag ttcaattggc tcgatcaagt aaaatttcgg ccgtatgaac
     1081 gttttcaaag gttttcattt ttttttaagt tcattcacaa tatcatgaat ctctcgtcct
     1141 ttggtttccg gtaagtagcg aaataatata agcatactta taaatgaagg aattgaatat
     1201 agaataagga aagcataact accaataacg gaatatagag gtaatactgt aaatgtactg
     1261 ataaccacca ttacagtatt cattgaatat gccacagatt gagcaatact acgatgtttt
     1321 tgaggtacca gttcagagga tataaaccac gaaattggac caactccagt tctgaaattt
     1381 cattattcga ttaaatcaaa atatatttga aaaacctcac ccataagtat aaccgtagat
     1441 gatcaatgca actaggcatc cgtatttcat cgggtcaatc agaatacgta tttttgcgaa
     1501 gaatacgaat gcaattagag ctaaattatt gagaaatgtg aagagaagga gaagcttgcg
     1561 tcgaccaact ctgtaaactt attcatacaa ttttgtcttc aaattttgaa cgtttaaaac
     1621 tatcttattc aatgagttag cgggttttta tagtgagtta gttcacgatt atctaataaa
     1681 actatgtcca aacatcctaa ctgcgaccgt attctcacta actcgtctta aaaacgttga
     1741 gattatttta aacccttctt cagtacaact acagtactga tgcacaaata caataataat
     1801 aatacattca gacatactct gaacaatgta acttgttttt gttatactgt ggtaacattt
     1861 attgaaatga aatataacct tacttttcaa tcacaattcc accagtaatt gttccagaaa
     1921 cataagctaa acccattgca gttgtactcc attcagcaat ttctgactca acatttgcat
     1981 tttctagaaa gaaagtagag gataaaagaa gagcccataa tccgacaggg ccctgtgaaa
     2041 ttgttatccg ctggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aagaccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctgaccccag
     3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4021 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4141 taactatgcg gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc
     4201 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     4261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tccccagtca cgacgtcaca
     4381 gcacgtgtgt ttttgaacac ggcgatatgt atctgtagat catccccaat tttcaacaat
     4441 gaatctaaat actgaacttt ttgtgtaatc tttagtgcag aaaaatgacc aaaatcagcg
     4501 attttccgat tatacccaaa atatctcagc tcaatgagca cctataaaaa tgtaaaaata
     4561 aagaaagtag tcgaaccata ttttgtaaag gctaaaaaac tgaataaatt tttatccatc
     4621 cagcgcggcg ttacaatccg tggcccatgt cacgcacggc tttcaaagaa aaaaaaacag
     4681 tctgtttgcg cgacatcgat ctggcaaaaa tcctaaaaac gactatgcgt ggctcacttt
     4741 atattaatat gaaaaataca aacttggtgg tgcaggtgct gcagaggcca tcgatgaaga
     4801 agtggaggcc ttgttgtcga tctacaaaat tgtatggttg tttgagattt tccttgaaac
     4861 gctctcaatc ttggatatga tataaattct ggataagata tgatggatat gatctatgtt
     4921 tgatagagtg catttgaaag gataacggcc ctgcagacat ttttgaaaaa ataacaaaca
     4981 tttaattttc tcactcgttt aggctattcc cctatttttg atattccttc gcacatatga
     5041 aaactacttt ttttcgaaac tgttaactcc agaatttata aatctatagc ccttacttga
     5101 ttatttatta tcatggttac tcacctcatg tctctttctc ttttcaagaa gtcttcgttc
     5161 atatctgttg tctctttcct ccttttgaat ctctgctttg ctcttcgcca ttgtttcctg
     5221 aaaataatgt aacttgaatt gtgtaatata cttttttaat ttgaatttgg cttgtaacgc
     5281 ggaatcacta cgtgcgggat catttcttac tagaaaaccc agaaaatgcc atatttcact
     5341 tatctcgggg tcatttctaa ttagaaaagc tacaaaaacc atttctaatt acggtgactt
     5401 tagaactggt cgaaatagct aattagaaat gggtttttgc taattagagg tgactctatg
     5461 ttatctgagt aagcctcgtt tgtatgtaaa ctcacagtac taataaatgc aagacacccg
     5521 ggtttgtcta gatatgaaat aattgaaata tcaattctga cagacaataa tggtaatctt
     5581 gataaggagt tccacgccca ggagaacacg ttagttttct tgtttttgat tgcgtgcgtt
     5641 attttggaga aaaactcgat tttttacaaa ataatttttt gaaaggaaca ctgttcaata
     5701 agttttgtct tttttctcag ttgtgatacg gttttttatt cttttttgta gttatacaga
     5761 agaccgttac gaaacagact gatatcgaaa caaacactga tcttacttgc acttataata
     5821 cgactcacta gtaggaacag ttatgtttgg tatattggga atgtattctg tcatttaagg
     5881 caaaaacccc atagacacta ctccactttc aaattatcaa cttctactca ccttccactg
     5941 agcctcaaac ccaaaccttc ttccgatctt tcttgcatcg tgctcatcaa tacttgtgat
     6001 tttagcaaga aaaacgccgt cctcgaaatc ttgacaaaca ataggggaaa ccccaaaaag
     6061 caaaagcagg aaaattaatt tcataaactt tcacttgatt gaaaaattcc aaaaatactc
     6121 caaaactttt tcacgatgag catgatttga cgtcatgaga ggcccagacg tgcgaagaaa
     6181 taaaaattgc ggtcataaac tgaaacgtaa catatgataa ggtattttgt gtgcggtttt
     6241 ttctatgatg tttcaaaaaa atttatcaga agtaaaaaac ataagaagtg tacacagaac
     6301 attgtgtaga ttttgggata gaattattat taatgggggg agggagcaca atttttttcg
     6361 actttttatt acgaattaat tctatttttg aatctacaca atttcattgt tagaggtgac
     6421 ttaaaagaag ctaaaaaaca aagaaattaa gagaagtttt ttgataataa caaaaatagg
     6481 gggtgggagc acagggagaa agagcatgta gggatgttga agagtaattg gacttagaag
     6541 tcagaggcac gggcgcgaga tggcgatctg atgacagcgg ccgatgcgga gcttcgcgat
     6601 gcgctagctc cacgcgttgc agatctttat tttttcgcgg gtgaaacgac tcctggtttg
     6661 tacaaagcca gcagttcatc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
     6721 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
     6781 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
     6841 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
     6901 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgcgccatg
     6961 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
     7021 aactctgctt ccgggaaata gggttttttg tagcggccga acggcacgtt aaactcaccg
     7081 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
     7141 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
     7201 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
     7261 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
     7321 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
     7381 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
     7441 aaaaaagcgc gatttttctt gccgggtgcc agatcctctt cagagatgag tttctgttcg
     7501 ccggcatccc ccgggcattt gtatagttca tccatgccat gtgtaatccc agcagctgtt
     7561 acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat
     7621 tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
     7681 tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag
     7741 ttctgaaaat ttaaatcaga ttagttagtt agtaaaatca tgtttaaact tacaactttg
     7801 attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc
     7861 aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta
     7921 ttaacaaggg tatcaccttc aaacttgact tcagcacctg aaaatttaaa tatgtatggt
     7981 tagttagtac cgaactgttt aaacttacgt gtcttgtagt tcccgtcatc tttgaaaaat
     8041 atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc
     8101 atatgatctg ggtatctcga gaagcattga acaccataac agaaagtagt gacaagtgtt
     8161 ggctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact tacccatgga
     8221 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
     8281 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
     8341 agaattggga caactccagt gaaaagttct tctcctttac tcattttttc taccggtacc
     8401 gccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
     8461 ccgatgagga ttttcgaagt tttttagatg cactagaaca aagcgtgttg gcttcctctg
     8521 agcccgcttt ccttatatac ccgcattctg cagccttaca gaatgttcta gaaggtccta
     8581 gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag acgcagacgg aaaatgtatc
     8641 tgggtctctt tattgtgtac actacttttc catgtaccga atgtgagtcg ccctcctttt
     8701 gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat agtataaata ccgttgaaaa
     8761 taaataccga acaacatttg ctctaattgt gaaattagaa atcttcaaac tataatcatg
     8821 tcactttacc actatttccg tccagctcaa cgttccgttt ttggtgatcc tctagagtcg
     8881 acctgcaggc atgcaagctt ggcggccgct acctgcagga tatctggatc cacgaagctt
     8941 cccatggtga cgtcaccggt tctagatacc taggtgagct ctggtaccct ctagtcaagg
     9001 ccttaagtga gtcgtattac gtagcccggg cctagttcta gacattctct aatgaaaaaa
     9061 tctttcagtt gaaattgaaa atgagttaaa gttggagttt ttattgaaaa cagatttccg
     9121 tgtgattagt gtttttagcg agtgtgacag gacagcgaaa aaatatagaa acaagggggg
     9181 aactgaaaag cttaggaatg cattgaacat gagaagggga aggggaagga acaaactaga
     9241 caggaattat tggaatttaa tcacatttgg agtttttttt ctattcgaca gaataattat
     9301 ccagaacatt tttgtattaa atatttatgc atcatatgag tagtcggctt tgttgtgcat
     9361 gacgagtttg ttatcgacga aatagaagct gtcagaacga gtctcgtttg gattgttgat
     9421 catgtcgtcc actgaaaaag agattagtct tttgaattgt actttttaga ataatgactc
     9481 actgagttgt tgagagagtt gagggaactc atagatatgt tcacagttgt ttcgtgaatt
     9541 cggaatacag aatccgaatt caaagtcaaa acacttcaga aggcgatctt tgaagaagtg
     9601 acgttcgatc attcggaaat gatggattgg gatgtctcct actttgaatt ccacagttgc
     9661 tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt atcttgccga
     9721 ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct cgaaaagcac
     9781 ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat agatgttcgc
     9841 agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa gggagcaaga
     9901 taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga gctttcttcg
     9961 caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt ggcatctgga
    10021 aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata tcaagagtta
    10081 gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg ccgagccggg
    10141 tggaatcgat cgttgttgct cggctttcat atcggtttgg ttggaagcgg ctgaaaacgg
    10201 aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt agagggtgcc
    10261 aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa aatacgataa
    10321 tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt tgagtacatg
    10381 taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt tttctattta
    10441 taacgctaat aatttgaaaa agaaacc
//
