LOCUS       sequence-171412-        6681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Morales-Martinez A, Dobrzynska A, Askjaer P
  TITLE     Inner nuclear membrane protein LEM-2 is required for correct nuclear
            separation and morphology in C. elegans.
  JOURNAL   J Cell Sci. 2015 Mar 15;128(6):1090-6. doi: 10.1242/jcs.164202. Epub
            2015 Feb 4.
  PUBMED    25653391
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.164202"; journalName: "J Cell Sci"; date: "2015-03-15- 
            15"; volume: "128"; issue: "6"; pages: "1090-6"
COMMENT     Sequence Label: pBN42
FEATURES             Location/Qualifiers
     source          1..6681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..35)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(189..777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(269..288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(948..1739,1740..1808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1571..1590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1809..1913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1981..1999
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2077..2096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2196..2218
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3094..3114)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     3862..3890
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(6468..6484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6468..6484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6481..6503)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6492..6508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6516..6522,6523..6540,6541..6546))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6561..6582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
       61 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1921 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1981 aataggcgta tcacgaggcc cttaaggccg cctgatgcgg tattttctcc ttacgcatct
     2041 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     2101 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     2161 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     2221 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcccg tacggccgac tagtagaaac
     2281 agttatgttt ggtatattgg gaatgtattc tgtcatttaa ggcaaaaacc ccatagacac
     2341 tactccactt tcaaattatc aacttctact caccttccac tgagcctcaa acccaaacct
     2401 tcttccgatc tttcttgcat cgtgctcatc aatacttgtg attttagcaa gaaaaacgcc
     2461 gtcctcgaaa tcttgacaaa caatagggga aaccccaaaa agcaaaagca ggaaaattaa
     2521 tttcataaac tttcacttga ttgaaaaatt ccaaaaatac tccaaaactt tttcacgatg
     2581 agcatgattt gacgtcatga gaggcccaga cgtgcgaaga aataaaaatt gcggtcataa
     2641 actgaaacgt aacatatgat aaggtatttt gtgtgcggtt ttttctatga tgtttcaaaa
     2701 aaatttatca gaagtaaaaa acataagaag tgtacacaga acattgtgta gattttggga
     2761 tagaattatt attaatgggg ggagggagca caattttttt cgacttttta ttacgaatta
     2821 attctatttt tgaatctaca caatttcatt gttagaggtg acttaaaaga agctaaaaaa
     2881 caaagaaatt aagagaagtt ttttgataat aacaaaaata gggggtggga gcacagggag
     2941 aaagagcatg tagggatgtt gaagagtaat tggacttaga agtcagaggc acgggcgcga
     3001 gatggcgatc tgatgacagc ggccgatgcg gagctccacc gatcccccgg gcatttgtat
     3061 agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg
     3121 tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct
     3181 ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga
     3241 acgcttccat cttcaatgtt gtgtctaatt ttgaagttct gaaaatttaa atcagattag
     3301 ttagttagta aaatcatgtt taaacttaca actttgattc cattcttttg tttgtctgcc
     3361 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
     3421 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
     3481 ttgacttcag cacctgaaaa tttaaatatg tatggttagt tagtaccgaa ctgtttaaac
     3541 ttacgtgtct tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct
     3601 tcgggcatgg cactcttgaa aaagtcatgc cgtttcatat gatctgggta tctcgagaag
     3661 cattgaacac cataacagaa agtagtgaca agtgttggct gaaaatttaa ataatcaggg
     3721 ttagttagta tatatatgtt taaacttacc catggaacag gtagttttcc agtagtgcaa
     3781 ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagaa
     3841 aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa
     3901 agttcttctc ctttactcat tttttctacc ggtaccttct agatggatct agtggtcgtg
     3961 ggtttgatgg ctccctgccc tgaaaattag acggtaaaag tggaagaaga agctggttga
     4021 caagcaaaga atccaaacga aatgtgagaa acaattgaaa taaaatagga aaaagaaaaa
     4081 tggaacactg cttttcagag ataaaaaagt tatagacatt gctataagtt tttggagaac
     4141 aatggtaaag cgtggtgaga agttttaggt ttcaatggga tttgaaagat gagagaaggt
     4201 attgaaaagg gacctcaatc atctttttgt gccgttttca tagcatcaaa aaccagaagc
     4261 atgcttcttc ttcttcttct tcttatgttt cttcttatcc tcccactccg gtaggctctc
     4321 ctccaatatc aattgaattg tattgagcct tcggagcgag ttgtggggga gcaacagaat
     4381 ctatacaaca cgcacacaca tcatcgttga agataaagaa gaagaaaacg gaatcccggg
     4441 agctatcccg ccctctctgc actctctctc taatattcta ttcttgctct tctattttca
     4501 cttcgggagc ccaaccaaca cctaccctcc ttcgttttcc gatgaacttg tgtttagaga
     4561 catagagata tcagagaagg gacacaaatt ttgtttttaa caaatcgccg agaaatttga
     4621 agaaaggtga tgaaaaactg ggtgctattg ttagccagtg gggaatgtaa ctgggaatac
     4681 cctagggtta tgaaactttt cagttcttac acaggtaaag ctggattttc caaatcatca
     4741 atcggttcct ctgcagtctc gattaaaata tttgatattt tcgtcaggga ttttgaagac
     4801 aaaaagttac ttttttcaaa aaaatgtaaa actgtaaaag gtaaaaattt aatagttgga
     4861 aatatgtgat aacagagacg tttacctgat ggcaggaggt gtgatccatt atattccatt
     4921 atattcgcgc actcttaaca ttacactagt gctatctgaa cagttgcgaa gaagatcaca
     4981 acaacagttt cagatttcaa gttttcctga ttgcgcaccg tgttgttcat ttaaccatgt
     5041 ttaaaattaa atatgaaata aggtgctttg aataattttt aacgatgctt gaaaaataac
     5101 ttcttctaca aaaatggtta atttctatgt acaatttttc ttttataaat tatactgact
     5161 ctcaaaccca tttttctgtt ctttgttttc tgtttttcat attttctcga cttgactgtt
     5221 ttgcaccccc tctgcaatca tttttgttga cccatacgtt gccatcacgt accaagtcca
     5281 agctatggta tctgttagca acagttgtca ctaatcacat ggctcttttt ctctgttttt
     5341 attcattttc ttcagtatct cgtgaagaaa acaatgtgtt gattggtctt tcaattcttg
     5401 caacagatgc attagattca gggcgccgga agtgcaagaa gtgggcgcta gtgaagaatt
     5461 gaaaaatatg ccaaacatct ggataaaaat aaaaaattaa cttgcagata aagaaaaaac
     5521 attgtggttg atcagaaatg ataattggtt ggaagccaaa ctttaacgtt ggttgaccaa
     5581 aaatacattg aactaattgg gttattcgtg aaattaaaac gtatatttca gtgcactttt
     5641 tgactaattg taacctgaaa aatatatatg tattccagaa cagtttttga ccttcttact
     5701 cttagaaatc ttaaatatgt attgaaaaat tgaaatgatc gtaacacagt cagtttggcc
     5761 atactatttt tttgggttct aaagtcagcg gagcgaagct gattgctgaa ttttttgagc
     5821 acaataccca atatgtactt cgtgagttcc gaaaacatat gaaatgtaac gaaacttgaa
     5881 gatctagttc atcacataat cctgaaaaca ctgattgtga aaaagcaatc acacacaaaa
     5941 ctcaaaatcg gccaccataa acctttttgc ggccagaccc ttttgaatta atctgtgtgc
     6001 ctttaagaac gcgtttcaga aattgcaaat atacaattct atatcattca aatagcatct
     6061 gttcaatgat cggtagtttc agaacatatt ccaaagatca attgtcattt tagaactgat
     6121 aattaaactt tcttcaccta cttctatttt caggagatta gtattttaag aactatgacg
     6181 tagtggaatg taaaactatg gcacgtgaca aattatgcag ttaattacag aataatctct
     6241 aattagcctg cacttttatt ttcgaaagaa atactcatgt tttgtcggga aaattatttt
     6301 attcaagaac ttattatagc cggcagtgat agagtacagg ttaaaataaa ttactgaaaa
     6361 tatatgtcaa cctgatcttt gttgcagcct tatcaagcaa aattcaaaat taacgaaaac
     6421 agagactata atcactcctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     6481 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6541 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6601 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6661 gggagaggcg gtttgcgtat t
//
