LOCUS       sequence-171414-        8669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for tandem expression of ATP1A1 G2 sgRNA in combination with 
            a user-specified sgRNA from two independent U6 promoters. Cloning of
            oligos for second sgRNA using BbsI sites. Px333-like plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, 
            Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y
  TITLE     Marker-free coselection for CRISPR-driven genome editing in human 
            cells.
  JOURNAL   Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265.
  PUBMED    28417998
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA
FEATURES             Location/Qualifiers
     source          1..8669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             243..263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(288..2825,2826..2828,2829..3290,3291..3293,3294..3461,
                     3462..3464,3465..4388)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4389..4436
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4464..4481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4470..4677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4686..4826
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4686..4815
                     /label=AAV2 ITR
     rep_origin      4901..5356
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4988..5007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5198..5219
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5373..5392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5492..5514
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5552..5570)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5638..5742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5743..5811,5812..6603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5961..5980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6774..7362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7263..7282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7424..7672
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        7691..7766
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7926..8174
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        8195..8270
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8284..8569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
       61 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      121 aattagctga gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt
      181 aattacctgg agcacctgcc tgaaatcact ttttttcagg ttggaccggt gccaccatgg
      241 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
      301 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      361 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      421 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      481 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      541 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      601 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      661 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
      721 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
      781 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
      841 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
      901 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
      961 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1021 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1081 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1141 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1201 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1261 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1321 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1381 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1441 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     1501 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     1561 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     1621 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     1681 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     1741 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     1801 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     1861 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     1921 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     1981 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2041 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2101 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2161 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2221 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2281 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2341 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2401 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2461 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     2521 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     2581 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     2641 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     2701 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     2761 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     2821 ttctggccga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     2881 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     2941 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3001 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3061 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3121 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3181 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3241 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct gcgctggaaa
     3301 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3361 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3421 tcaagaccga gattaccctg gccaacggcg agatccggaa ggcgcctctg atcgagacaa
     3481 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     3541 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     3601 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     3661 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     3721 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     3781 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     3841 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     3901 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     3961 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4021 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4081 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4141 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4201 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4261 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4321 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4381 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag
     4441 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4561 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4621 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg
     4681 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4741 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4801 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     4861 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     4921 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     4981 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5041 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5101 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5161 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5221 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     5281 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5341 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5401 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5461 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5521 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5581 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5641 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5701 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5761 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5821 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5881 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5941 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6001 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6061 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6121 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6181 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6241 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6301 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6361 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6421 tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca
     6481 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6541 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6601 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6661 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6721 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6781 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6841 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6901 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6961 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7021 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7081 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7141 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7201 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7261 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7321 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7381 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtgagggcc tatttcccat
     7441 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     7501 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     7561 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     7621 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc
     7681 gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     7741 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa
     7801 ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagctgta
     7861 caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc aaggtcgggc
     7921 aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     7981 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     8041 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     8101 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     8161 aaggacgaaa caccgatcca agctgctaca gaaggtttta gagctagaaa tagcaagtta
     8221 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaag
     8281 ctccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8341 attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8461 tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga
     8521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     8581 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     8641 atttattttt taattatttt gtgcagcga
//
