LOCUS       sequence-171430-        9278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hsOPA1 isoform 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Song Z, Chen H, Fiket M, Alexander C, Chan DC
  TITLE     OPA1 processing controls mitochondrial fusion and is regulated by 
            mRNA splicing, membrane potential, and Yme1L.
  JOURNAL   J Cell Biol. 2007 Aug 27;178(5):749-55. Epub 2007 Aug 20.
  PUBMED    17709429
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2007-08-27"; volume: "178(5)"; pages: "749-55. Epub 
            2007 Aug 20"
COMMENT     Sequence Label: OPA1-isoform7
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..333
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(3417..3833)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3433..3449)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3465..3487)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3900..4241
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             4305..4821
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5021..5040)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5168..5190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5384..5403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5503..5525
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5563..5581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5649..5753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5754..5822,5823..6614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5972..5991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6785..7373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7274..7293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7527..7544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7661..7682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7697..7702,7703..7720,7721..7727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7735..7751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             7913..8427
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(8527..9126)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8610..8630)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9107..9126
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9147..9278,1..368))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9195..9214)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc
       61 gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact
      121 agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg
      181 gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagc
      241 cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg
      301 ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc
      361 ccctacccgg tagaattcgt tggccgctta tttctcctga tgaagagctt caatgaaagc
      421 atcaagtttt tcttgaattt ctctaacttt cttgaggtct tccgccagtt gaacgcgttt
      481 accagtaagc aatttaatct tcttctcacc atcttcagca aaatcttcca atacctcttt
      541 aacatttttc tctaatcgcc taacttcagt atttgtaagt tgttgcctta aagtatttgc
      601 ggtgatagca agcatgcgct gtatacgcca aaacaagacc acatcattgc attccaactc
      661 agaatctaca aaatgccttt ggtagtaata aaaacctctt cgacaaaggt tacaatggtt
      721 tagagctgtt tttaaaaaat gtcttctata aacttgatgc caagtatcct taatcaagct
      781 tggatctact tctactcctc gggattcaag gttcttccgg actgtggtta tttcatcact
      841 tgcaagataa gctgggtgct cctcattaca tttcaacatc ttctccaatt cattcttggt
      901 ttcattgtga acacactgtt cttgggtccg attcttccag tataaccacc tctttttcca
      961 gtctggaccc accatgtttt caattgcatt ttcagtatcc ttgagacgag cctgcagagc
     1021 ctcttccata aaataaatag ctgcatccca ttgctgttta tcagatatgg atcggtcttc
     1081 caaagcattg tgttgaataa ccctcaagct gtcctccgca aagtcattcc acttgtgtcg
     1141 tttaatactt tcttccttaa cggcctcttt aagtttatca aatatgtcat catgctcttt
     1201 ccctttcggt tctgtcataa agcgggaaaa ttcttcttgt agggtctccc aagcaacctc
     1261 tactgcttta ttaggaagtt gtttatcagt ccactgttta agcttgatat ccactgtggt
     1321 gttaaaagtt cctgaattca tggtctgcgc agctggaagg tagatgtttt caatcacatg
     1381 agttgatact ctttcccaca aagattgttg aaggatttcc tcccaatgtt ttggtgtaac
     1441 ctggctcaga ctgataactt catcaaggat ttcattttta gctttttcaa atagttcatt
     1501 ccggtcaagt tcccgcaggc gaggatagtt attcttccat tcagtttcaa ggttaaaacg
     1561 tgttgctttg aaactatcag cctgttgttc aacagactct cgtaccattt tccaaaagca
     1621 gtctgatact gcaaggctta aatttcttgt agtcacttgg tgtgccttta gcatgcttgt
     1681 ctttaggagc tttgaattct gaaaaaactc ttcttcatat tctcttatag cttcaatgct
     1741 ttcagagctg ttcccttttc ctgttacaac agcaaaataa cctaaagctt tcattgggaa
     1801 gagctttcct tcaattatct gctgaatcct gcttggactg gctacatttt tctctgccag
     1861 gtctactttg gtcaaaacga atatggttct ccttccatga gggtccattt gactgaccaa
     1921 gtctgtaaca atactgcgtt cagcatccac agatccatct tgaatacaca gtatgatggc
     1981 attaggattc tgcatgtaag ctttgctgat actgaaaata gtttcctttg tgtcaggagc
     2041 catgcctgat gtcacagtat taatcacacc tggtaagtca acaagcacca tcctctgtag
     2101 tccagggcct tttacattta aggatatggt ctcagggcta acggtacagc cttctttcac
     2161 atttttcctc attcgaagtt ctatttcatg tcttaatgct gcaagatctt cttctttggt
     2221 aagatcaaac tcccgagaac tatctttaaa tagggccaca tggtgaggac cttcactcag
     2281 agtcacctta actggagaac gtgtcatcat ctccccagat cctcttggga atattcgagc
     2341 ttgggcaatc atttccaaca cactagtctt tccagcactc tgatctccaa ccacaacaac
     2401 ccgtggcaga tgatcttgcg tattataact ggcatcataa tcagagagaa catcaagaac
     2461 ttcagaatac atgtcaatca aagatttctt aagctttcta tgatgaatgc ctttgtcatc
     2521 tttctgcaat actaattttc tcaattcttt gttctccttt tctaatcgtt ccaagattct
     2581 ctgatacttc aactgagtgt gcagaagttc ttcctgaagt tggtcaattt tctctttgtc
     2641 tgacaccttg cgcttctgtt gggcatagct cgtgctatat tggccagcgg ctctgcgcgc
     2701 ttcctcttca tgctcttgaa tttgttgttg taagagaatg agctcaccaa gcagaccctt
     2761 tctaaaatgc ttgtcacttt cagatccacg atctgttgct ctaaacgccg tttcttccgg
     2821 agaacctgag gtaaaaaagt ccttcaataa gctaaggctt tcaacaatct tgtcaaagtc
     2881 tggtgctaac tttacaaggt cttctgaatt aggaagggct tttctaattt tctcaaaatc
     2941 gatatactca tcaatttccc acacaatgtc aggcacaatc catttatatt cactaaggtc
     3001 cggtatcata tctttccact gatcaaaagt ctttttggct gtgtagccac ccccaacagc
     3061 cgatcctagt atgagatagc gaagttttaa gagtctcgta gctaatcttg ctggccaaaa
     3121 attcctgcga ggctggtagc catatttaat tggagagaat ttcagtttac gtaaaggaag
     3181 gtttgtcaga gaagagaact gctgaaagga tgtccttaat tggggtcgtt gaagctttaa
     3241 ggtaggatga tgtgaatgat aaatgcttcg tgaaaccaga tgtagttttt gtagtggtaa
     3301 acttcctttt attccagagc tgtgtttcac taaagactgg cagacctcac aggccacagc
     3361 ggcccgacgt agtcgccaca tggtaccacg cgtgaattct cgagagatct aattccggcg
     3421 cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt
     3481 tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag
     3541 ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc
     3601 tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg
     3661 tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag
     3721 cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg
     3781 acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt
     3841 tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc
     3901 agcagacaag acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg
     3961 aactgtttta ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac
     4021 atcgactccc ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc
     4081 ccagggttgc ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag
     4141 agctagttag ctaactagta cagacgcagg cgcaaacatt agatgccggc acaaacacgc
     4201 acaaagacag agacagacac gaaacgaccg ctggccagct tacctcccgg cgggggggtc
     4261 ggtggtccct gggcaggggt ctccaaatct cggtggaacc tccaaatgaa agacccccga
     4321 ggtgggcagt caatcaatct gaggagaccc tcccaaggat cagcgagtcc acgattcgga
     4381 tgcaaacagc aagaggcttt attgggaata cgggtacccg ggcgacgcag tctatcggag
     4441 gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc
     4501 gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca
     4561 ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga gggcgggacc
     4621 gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt accacagata
     4681 tcctgtttgg cccatattct gctgtctctc tgttcctaac cttgatctga acttctctat
     4741 tctcagttat gtattttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac
     4801 ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt
     4861 atctatggct cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc
     4921 tggaccactg atatcctgtc tttaacaaat tggactaatc gcctctacgc cggacgcatc
     4981 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     5041 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     5101 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     5161 gcgccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5221 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5281 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     5341 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     5401 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5461 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     5521 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     5581 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5641 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5701 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5761 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5821 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5881 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5941 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6001 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6061 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6121 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6181 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6241 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6301 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6361 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6421 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6481 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6541 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6601 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6661 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6721 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6781 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6841 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6901 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6961 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7021 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7081 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7141 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7201 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7261 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7321 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7381 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7441 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7501 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7561 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7621 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7681 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7741 gcggataaca atttcacaat tctccccagc tctttctgcg ctgtccataa tgaactattt
     7801 caggagtatt ggagtatgtg gattcaaaac agtggctaat tgagtcagga ttacttaata
     7861 ttcgactcct accctcagaa tgttgttctc caacttcaag aaactgttac ccatgaaaga
     7921 cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac
     7981 aagtcggatg caactgcaag agggtttatt ggatacacgg gtacccgggc gacgcagtct
     8041 atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc tcggggagca
     8101 gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt
     8161 catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg
     8221 cgggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc
     8281 acagatatcc tgtttggccc atattctgct gtctctctgt tcctaacctt gatctgaact
     8341 tctctattct cagttatgta ttttccatgc cttgcaaaat ggcgttactt aagctagctt
     8401 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
     8461 ttattttatc gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg
     8521 cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
     8581 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
     8641 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
     8701 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
     8761 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
     8821 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
     8881 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
     8941 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
     9001 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
     9061 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
     9121 ggtcatggta agcttgggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
     9181 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
     9241 cccgccccgc ccctgagccc gcccctgagc ccgccccc
//
