LOCUS       sequence-171433-       11761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression construct encoding SR-B1 and GFP from separate
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11761)
  TITLE     Grove lab plasmids
REFERENCE   2  (bases 1 to 11761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDUAL SRBI (GFP)
FEATURES             Location/Qualifiers
     source          1..11761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2065..2182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2335..2742
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2782..2791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4413..4433)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4831..4842)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5421..5439
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5517..5526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5523..5525,5526..5528,5529..6242)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5523..5525,5526..5528,5529..6242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5568..5589)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5829..5848)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6176..6197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6338..6571
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6596..6614)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6597..6614)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6904..6923)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7137..7156)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7256..7278
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7316..7334)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7402..7506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7507..7575,7576..8367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7725..7744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8538..9126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9027..9046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9280..9297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9367..9387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9372..9701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9552..9687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10835..10900
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11030..11050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11475..11609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11512..11531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11566..11585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2041 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2101 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2161 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     2221 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     2281 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     2341 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     2401 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     2461 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     2521 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     2581 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     2641 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     2701 tcactcggcg cgccagtcct ccgacagact gagtcgcccg gggggatcca ctagtgcggc
     2761 cgcgactcta gagtcgacgc cgccaccatg ggctgctccg ccaaagcgcg ctgggctgcc
     2821 ggggcgctgg gcgtcgcggg gctactgtgc gctgtgctgg gcgctgtcat gatcgtgatg
     2881 gtgccgtcgc tcatcaagca gcaggtcctt aagaacgtgc gcatcgaccc cagtagcctg
     2941 tccttcaaca tgtggaagga gatccctatc cccttctatc tctccgtcta cttctttgac
     3001 gtcatgaacc ccagcgagat cctgaagggc gagaagccgc aggtgcggga gcgcgggccc
     3061 tacgtgtaca gggagttcag gcacaaaagc aacatcacct tcaacaacaa cgacaccgtg
     3121 tccttcctcg agtaccgcac cttccagttc cagccctcca agtcccacgg ctcggagagc
     3181 gactacatcg tcatgcccaa catcctggtc ttgggtgcgg cggtgatgat ggagaataag
     3241 cccatgaccc tgaagctcat catgaccttg gcattcacca ccctcggtga acgtgccttc
     3301 atgaaccgca ctgtgggtga gatcatgtgg ggctacaagg acccccttgt gaatctcatc
     3361 aacaagtact ttccaggcat gttccccttc aaggacaagt tcggattatt tgctgagctc
     3421 aacaactccg actctgggct cttcacggtg ttcacggggg tccagaacat cagcaggatc
     3481 cacctcgtgg acaagtggaa cgggctgagc aaggttgact tctggcattc cgatcagtgc
     3541 aacatgatca atggaacttc tgggcaaatg tggccgccct tcatgactcc tgagtcctcg
     3601 ctggagttct acagcccgga ggcctgccga tccatgaagc taatgtacaa ggagtcaggg
     3661 gtgtttgaag gcatccccac ctatcgcttc gtggctccca aaaccctgtt tgccaacggg
     3721 tccatctacc cacccaacga aggcttctgc ccgtgcctgg agtctggaat tcagaacgtc
     3781 agcacctgca ggttcagtgc ccccttgttt ctctcccatc ctcacttcct caacgccgac
     3841 ccggttctgg cagaagcggt gactggcctg caccctaacc aggaggcaca ctccttgttc
     3901 ctggacatcc acccggtcac gggaatcccc atgaactgct ctgtgaaact gcagctgagc
     3961 ctctacatga aatctgtcgc aggcattgga caaactggga agattgagcc tgtggtcctg
     4021 ccgctgctct ggtttgcaga gagcggggcc atggaggggg agactcttca cacattctac
     4081 actcagctgg tgttgatgcc caaggtgatg cactatgccc agtacgtcct cctggcgctg
     4141 ggctgcgtcc tgctgctggt ccctgtcatc tgccaaatcc ggagccaaga gaaatgctat
     4201 ttattttgga gtagtagtaa aaagggctca aaggataagg aggccattca ggcctattct
     4261 gaatccctga tgacatcagc tcccaagggc tctgtgctgc aggaagcaaa actgtaggtc
     4321 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     4381 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4441 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4501 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4561 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4621 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4681 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4741 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4801 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4861 cgcggtcgaa ttcccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     4921 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     4981 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     5041 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     5101 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     5161 cagggagcar kggcagcscg accgcgatcc gaaggttcct tgcggtkcgc ggcgtgccgg
     5221 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     5281 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gtggctgctc agcagggcgc
     5341 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     5401 gccctgttcc tgcccgcacg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     5461 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc aggacgcgta ccggtcgcca
     5521 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5581 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5641 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5701 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5761 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5821 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5881 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5941 acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga
     6001 acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg
     6061 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6121 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6181 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6241 aaactcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     6301 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6361 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6421 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6481 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6541 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     6601 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     6661 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     6721 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     6781 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     6841 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     6901 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     6961 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     7021 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     7081 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     7141 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7201 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7261 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     7321 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7741 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8041 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9301 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9361 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9421 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9601 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     9721 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     9781 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     9841 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     9901 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     9961 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
    10021 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
    10081 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
    10141 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
    10201 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
    10261 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
    10321 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
    10381 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
    10441 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
    10501 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
    10561 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
    10621 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
    10681 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
    10741 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
    10801 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
    10861 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
    10921 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
    10981 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
    11041 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
    11101 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
    11161 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
    11221 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
    11281 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
    11341 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
    11401 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    11461 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11581 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11641 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11701 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11761 c
//
