LOCUS       sequence-171438-        8481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline inducible expression of shRNA targeted to AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de
            Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella 
            L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha 
            S, Kotter M, Vallier L
  TITLE     Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro 
            studies of human development using hPSCs.
  JOURNAL   Development. 2016 Dec 1;143(23):4405-4418.
  PUBMED    27899508
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-12-1"; volume: "143(23)"; pages: 
            "4405-4418"
COMMENT     Sequence Label: pAAV-Puro_siKD
FEATURES             Location/Qualifiers
     source          1..8481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     220..242
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     304..323
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             367..387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1041..1152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1282..2118
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(2152..2171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2153..2171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2178..2195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2178..2194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2187..2209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2332..2634
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2513..2532
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             2983..3777
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2983..3777
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3037..3056)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3647..3666
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(4027..4818,4819..4887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4650..4669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5011..5599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5500..5519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5753..5770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5887..5908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5923..5928,5929..5946,5947..5953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5961..5977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5966..5988
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5985..6001
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5985..6001
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6022..6040
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6075..6878
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    6885..6910
                     /label=SA
                     /note="splice acceptor site"
     CDS             6934..6987
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6997..7596
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6997..7016)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7493..7513
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7634..7858
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8002..8021
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    8067..8085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(8097..8113)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8098..8114)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     enhancer        8176..8479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
       61 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
      121 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
      181 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
      241 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
      301 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatta attcggatcc
      361 accatgccca agaaaaagcg gaaggtgtcc cggctggaca agagcaaagt gatcaacagc
      421 gccctggaac tgctgaacga agtgggcatc gagggcctga ccacccggaa gctggcccag
      481 aaactgggcg tggaacagcc caccctgtac tggcacgtga agaacaagcg ggccctgctg
      541 gacgccctgg ccatcgagat gctggaccgg caccacacac acttttgccc cctggaaggc
      601 gaaagctggc aggacttcct gcggaacaac gccaagagct tcagatgcgc cctgctgagc
      661 caccgggacg gcgccaaagt gcacctgggc accagaccca ccgagaagca gtacgagaca
      721 ctggaaaacc agctggcctt cctgtgccag cagggcttca gcctggaaaa cgccctgtac
      781 gccctgagcg ccgtgggcca ctttaccctg ggctgcgtgc tggaagatca ggaacaccag
      841 gtcgccaaag aggaaagaga gacacccacc accgacagca tgccccccct gctgagacag
      901 gccatcgagc tgttcgatca tcaaggcgcc gagcccgcct tcctgttcgg cctggaactg
      961 atcatctgcg gcctcgagaa gcagctgaag tgcgagagcg gctcccccag atgaacgcgt
     1021 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     1081 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     1141 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     1201 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctgggggtc tcgagggggg
     1261 gcccggacag tactaagctt tactagggac aggattggtg acagaaaagc cccatcctta
     1321 ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg ttaggcagat
     1381 tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc cagaacctct
     1441 aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg cagggggtgg
     1501 gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct
     1561 cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc
     1621 agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg
     1681 gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg
     1741 tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg
     1801 tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt
     1861 caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag
     1921 tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc
     1981 cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc
     2041 ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct tccctacagg
     2101 ggttcctggc tctgctctaa gggcgaattc gcggccgcta aattcaattc gccctatagt
     2161 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2221 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2281 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg gcagtttaag
     2341 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     2401 attgacacgc cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     2461 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     2521 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     2581 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     2641 ggcatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg
     2701 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     2761 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     2821 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     2881 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     2941 ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     3001 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3061 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3121 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     3181 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     3241 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     3301 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     3361 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     3421 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     3481 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     3541 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     3601 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     3661 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     3721 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
     3781 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3841 caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3901 ctaaatacat tcaaatatgt atccgctcat gagattatca aaaaggatct tcacctagat
     3961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4141 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4561 cttttctgtg actggtgaat actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4921 tcagggttat tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5761 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5821 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5881 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     5941 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6001 ccatgattac gccaagctca gaattaaccc tcactaaagg gactagtcct gcaggtttaa
     6061 acgaattcgc cctttgcttt ctctgaccag cattctctcc cctgggcctg tgccgctttc
     6121 tgtctgcagc ttgtggcctg ggtcacctct acggctggcc cagatccttc cctgccgcct
     6181 ccttcaggtt ccgtcttcct ccactccctc ttccccttgc tctctgctgt gttgctgccc
     6241 aaggatgctc tttccggagc acttccttct cggcgctgca ccacgtgatg tcctctgagc
     6301 ggatcctccc cgtgtctggg tcctctccgg gcatctctcc tccctcaccc aaccccatgc
     6361 cgtcttcact cgctgggttc ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt
     6421 tcttaggatg gccttctccg acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg
     6481 cctgcatcat caccgttttt ctggacaacc ccaaagtacc ccgtctccct ggctttagcc
     6541 acctctccat cctcttgctt tctttgcctg gacaccccgt tctcctgtgg attcgggtca
     6601 cctctcactc ctttcatttg ggcagctccc ctacccccct tacctctcta gtctgtgcta
     6661 gctcttccag ccccctgtca tggcatcttc caggggtccg agagctcagc tagtcttctt
     6721 cctccaaccc gggcccctat gtccacttca ggacagcatg tttgctgcct ccagggatcc
     6781 tgtgtccccg agctgggacc accttatatt cccagggccg gttaatgtgg ctctggttct
     6841 gggtactttt atctgtcccc tccaccccac agtggggcaa gcttctgacc tcttctcttc
     6901 ctcccacagg gcctcgagag ttctggcagc ggagagggca gaggaagtct tctaacatgc
     6961 ggtgacgtgg aggagaatcc cggccctagg ctcgagatga ccgagtacaa gcccacggtg
     7021 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7081 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7201 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7321 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7561 cgcacctggt gcatgacccg caagcccggt gcctgatcta gagggcccgt ttaaacccgc
     7621 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7681 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7741 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7801 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt
     7861 caattcgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg
     7921 cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg
     7981 gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg
     8041 tctttggatt tgggaatctt ataagttccc tatcagtgat agagatctaa gcttatcgat
     8101 accgtcgacc tcgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     8161 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     8221 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     8281 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8341 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     8401 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     8461 agtcatcgct attaccatgg t
//
