LOCUS       sequence-17144-e         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill 
            G, Howley PM
  TITLE     The hPLIC proteins may provide a link between the ubiquitination 
            machinery and the proteasome.
  JOURNAL   Mol Cell 2000 Aug;6(2):409-19.
  PUBMED    10983987
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-08"; volume: "6(2)"; pages: "409-19"
COMMENT     Sequence Label: p4458 FLAG-hPLIC-1
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     39..56
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata
       61 caagctactt gttctttttg caggatccgc caccatggac tacaaggacg acgatgacaa
      121 agatcccccc ccaagcttca tggccgagag tggtgaaagc ggcggtcctc cgggctccca
      181 ggatagcgcc gccggagccg aaggtgctgg cgcccccgcg gccgctgcct ccgcggagcc
      241 caaaatcatg aaagtcaccg tgaagacccc gaaggaaaag gaggaattcg ccgtgcccga
      301 gaatagctcc gtccagcagt ttaaggaaga aatctctaaa cgttttaaat cacatactga
      361 ccaacttgtg ttgatatttg ctggaaaaat tttgaaagat caagatacct tgagtcagca
      421 tggaattcat gatggactta ctgttcacct tgtcattaaa acacaaaaca ggcctcagga
      481 tcattcagct cagcaaacaa atacagctgg aagcaatgtt actacatcat caactcctaa
      541 tagtaactct acatctggtt ctgctactag caaccctttt ggtttaggt
//
