LOCUS       sequence-171443-        4628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of HA tagged Xenopus laevis Nup53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, 
            Sieverding C, Madlung J, Gerken U, Macek B, Antonin W
  TITLE     Dimerization and direct membrane interaction of Nup53 contribute to 
            nuclear pore complex assembly.
  JOURNAL   EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. 
            Epub 2012 Sep 7.
  PUBMED    22960634
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.256"; journalName: "EMBO J"; date: "2012-10-17- 
            17"; volume: "31"; issue: "20"; pages: "4072-84"
COMMENT     Sequence Label: pSI HA Nup53xl
FEATURES             Location/Qualifiers
     source          1..4628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1..192
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(24..44)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          428..560
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     543..562
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        605..623
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             632..658
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1687..1808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1698..1717)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1752..1771
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1989..2444
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2076..2095)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2286..2307
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2511..2530)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2630..2652
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2690..2708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2776..2880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2881..2949,2950..3741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3099..3118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3912..4500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4401..4420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
       61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gcttgattct tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac
      421 tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt
      481 gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca
      541 ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag
      601 tacttaatac gactcactat aggctagcat gtacccatac gacgtcccag actacgctat
      661 gatggcagca gcatttagca tggaaccgat gggtgcagaa ccgatggcac tgggtagccc
      721 gaccagcccg aaaccgagtg ccggtgcaca gtttctgcct ggttttctgc tgggtgatat
      781 tccgacaccg gttacaccgc agcctcgtcc gagcctgggt attatggaag ttcgtagtcc
      841 gctgcatagc ggtggtagtc ctccgcagcc ggttctgccg acccataaag ataaaagcgg
      901 tgcacctccg gttcgtagca tttatgatga tgttgcaagt ccgggtctgg gtagcacacc
      961 gcgtaatacc cgtaaaatgg caagctttag cgttctgcat acacctctga gcggtgcaat
     1021 tccgagcagt ccggcaagca atgtttttag tccggcaacc attggtcaga gccgtaaaac
     1081 caccctgagt ccggcacaga tggacccgtt ttatacccag ggtgatgcac tgaccagtga
     1141 tgatcagctg gatgatacct gggttaccgt ttttggtttt ccgcaggcaa gcgcaagcta
     1201 tattctgctg cagtttgcac agtatggcaa tattattaaa catgtgatga gcaataatgg
     1261 caattggatg catattcagt atcagagcaa actgcaggca cgtaaagcac tgagcaaaga
     1321 tggtcgtatt tttggtgaaa gcattatgat tggtgtgaaa ccgtgcattg ataaaagcgt
     1381 tatggaagca accgaaaaag ttagcacccc gagcgttagc agcgttttta cacctccggt
     1441 taaaagcatt cgtaccccga cccagagcgt tggtacaccg cgtgcagcaa gcatgcgtcc
     1501 gctggcagca acctatcgca ccccgaccag cgcagattat caggttgtta gcgataaacc
     1561 ggcaccgcgt aaagatgaaa gcattgttag caaagccatg gaatatatgt ttggttggtg
     1621 atagctcgag aattcacgcg tggtacctct agagtcgacc cgggcggccg cttcgagcag
     1681 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1741 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1801 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1861 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct
     1921 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1981 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2341 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2401 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     2461 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     2521 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2581 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2641 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2701 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2761 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2881 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3001 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3061 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3121 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3421 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3481 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3541 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3721 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3781 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4021 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4561 gctcgacaga tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
     4621 ggtacctt
//
