LOCUS       sequence-17145-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill 
            G, Howley PM
  TITLE     The hPLIC proteins may provide a link between the ubiquitination 
            machinery and the proteasome.
  JOURNAL   Mol Cell 2000 Aug;6(2):409-19.
  PUBMED    10983987
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-08"; volume: "6(2)"; pages: "409-19"
COMMENT     Sequence Label: p4459 FLAG-hPLIC-1 NTF
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgctcgt ttagggaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat ggactacaag
      121 gacgacgatg acaaagatcc ccccccaagc ttcatggccg agagtggtga aagcggcggt
      181 cctccgggct cccaggatag cgccgccgga gccgaaggtg ctggcgcccc cgcggccgct
      241 gcctccgcgg agcccaaaat catgaaagtc accgtgaaga ccccgaagga aaaggaggaa
      301 ttcgccgtgc ccgagaatag ctccgtccag cagtttaagg aagaaatctc taaacgtttt
      361 aaatcacata ctgaccaact tgtgttgata tttgctggaa aaattttgaa agatcaagat
      421 accttgagtc agcatggaat tcatgatgga cttactgttc accttgtcat taaaacacaa
      481 aacaggcctt gttacagatt cagcagggtt tacagacatt agcaacggaa gccccgggcc
      541 tcatcccagg gtttactcct ggcttggggg cattaggaag cactggaggc tcttcgggaa
      601 ctaatggatc taacgccaca cctagtgaaa acacaagtcc cacagcagga accactgaac
      661 ctggacatca gcagtttatt cagcagatgc tgcaggctct tgctggagta aatcctcagc
      721 tacagaatcc agaagtcaga tgactcgagc ctctagaact atagtgagtc gtattacgta
      781 gatccagaca tgataagata
//
