LOCUS       sequence-171468-       13673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of human Plexin-B2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, 
            Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, 
            Dai G, Zou H, Friedel RH
  TITLE     Plexin-B2 facilitates glioblastoma infiltration by modulating cell 
            biomechanics.
  JOURNAL   Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
  PUBMED    33514835
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-01-29"; volume: "4(1):145. doi"; pages: " 
            10.1038/s42003-021-01667-4"
COMMENT     Sequence Label: pLV-PLXNB2
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     315..338
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    416..1004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             522..533
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     931..951
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1035..1059
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6604..6628)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6683..7193)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6746..6764)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7239..7356
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7623..7667)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7852..8085
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8578..8703
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8750..8930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8931..9157)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9185..9205)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9185..9203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9224..9240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9224..9240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9237..9259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9248..9264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9272..9278,9279..9296,9297..9302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9317..9338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9455..9472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9626..10214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9706..9725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10385..11176,11177..11245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11008..11027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11246..11350)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11376..11831)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11513..11534)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11725..11744
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11958..11980
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11972..11989
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11973..11989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11999..12018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11999..12017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      12038..12173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12213..12334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12224..12243)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12278..12297
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             12406..12639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(12730..13533)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12841..12860)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13451..13470
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(13547..13673,1..373))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13595..13614)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
       61 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
      121 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
      181 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
      241 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
      301 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
      361 gcctccccta cccggtagaa ttagatcact agtccggata tctgcagaat tccaggcggg
      421 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
      481 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
      541 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag
      601 gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
      661 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
      721 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      781 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      841 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      901 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
      961 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgag cggccgccac
     1021 tgtgctggat atcaaccact ttgtacaaga aagctgggcc catgaggccc tagaggtcag
     1081 tgaccttgtt ctccagtgca gcggcaatct gctgcaggcg gaaggccagc tgcatcttct
     1141 gggcggcagg atcctcctcc aaggcattga tgatctcgtc atagtacttc tgcgtgtatt
     1201 ggtagagctg gtggagtgcc acgagggtgt tcaaggagtc cgtgtgcgcc cgggaaatct
     1261 ctgccaggtg tgtgttcatg tcctggtcgc tgacctgcac catctgccgg atccccttgt
     1321 agtaatcctc caccatcttc ttgtaggtgg agatctcctt ggcgtacagc agcttgttgc
     1381 tgggagaatc gcggctcagc ttatgctccg tgcgcgtgca ggcatccatg aaggtctgcg
     1441 cgatgactga cagcgaggcg tccaccacct cgtggacatg cacgtcaaag atgaagtggg
     1501 ggttcttgag gatgttcacc cagaaccgga gcggtaagct gttcgtcttc cagatgtgga
     1561 tggtgtcttc atcctggatg ttgtgcttct ctgcctgctc gtccaggaag tcgaagaagt
     1621 acttgactgc aggtggcacc gcgtgcccag gcgccagcac gctctggaag aagttgtcca
     1681 caaactgctg cagtgtgccc ttgactgaga gcagccgcgt caggtagatc tcggtgatgg
     1741 ccttcgtccg ctccttctct ttcacgctgc ctctcttgga cttgccctcg tccacctcgt
     1801 cggtcggccg caccaggtgc cacacccggt tctcctcctc caggagggca tggcgctccc
     1861 caggcaggtc ctgctggctg tcctccggct gctgggagac ccccaccttg gacaggatga
     1921 gggtggctcc atcccggaca ttgtagtgca taagggtgtt gacgcgcttc caccggccct
     1981 cccgctgtga cgtcaggtcc aggtccgaca ggatctgcgc tgtggagccc ggacgccact
     2041 ccaggaccac gctgtctggc ctgggccagc aggagcaggg ctgcccacgg tacacctggt
     2101 caatgatctt ctccttgacc tgggagatgg tgtcacagtt gaggaccttc accgggatgg
     2161 cgtccactcc ctcgtcctgc acgatcacgc tcaccgtcag gggtgcgtac tccacatcat
     2221 cccccagcag ccccgtgtcg ttgagagtgt acttggcctt cttctgtacc gcatccaccg
     2281 ggcccttttc cacctgatgt ttgatggcct tgaagagctt gtacaggggc tccccggcac
     2341 tgtccttgag gtactggtac aggcagatgg acatccagtt ggacagcatc ctctccacca
     2401 cagtctcaga cctgcgcagc atcagcttgg ggttcttggc caccacgtac tgctccagga
     2461 gctccaggaa gagcgtgtgc atgatgtccg tgtagtactc cagtttcccg tgcagcgcca
     2521 ccgtcagcag ggacgcgaag tagaccttgg cgcgggccga gaactcccgc tggttctcca
     2581 gggtgtggat gaaattgatg aggaaagact tgctgttcag caggttggag aactggtaga
     2641 gggcctgctc caccaccggc cgccgcggct cagggatgtc cagcttgccg gtgatcatca
     2701 cgtccttgtc gccgtccttg gagggcagga agaagacgcg gtcggtgtag gtcttgtagt
     2761 ccagcacggg gatgccggcc tcgtgcacgt cgttggtctg gtcctccatc tcgatcatca
     2821 ggtctgtgaa ttccttcttg cagcggtccc gcacgctctc ctccaggccc tccagctggg
     2881 acttgatctt ctcatactct cgttcggcct gctggctctt cctccagtag cagtagacag
     2941 acaccgcgat gacgaccacc atgggcacga tgaccagcgg caagatgagg ctgagcggca
     3001 cgtcgctcac ccgtgtgtcg tactccacgc ggcccagcac ccactcgcga gagccgaact
     3061 tcacaatgaa ctcgggcagg ttgtgtgtgg tgtctcgttt ctgccgccgc ttgggcgggg
     3121 gctgcacctc cgggggctca cagtacaggt cggtctccgt cagcgtcttc atggtgcagc
     3181 gctcggcacc cacgaaggcc tcggcctcct gcagcgtcat cgccttgttc agattggtgc
     3241 cccgggcgtg gatgagcttg ttgacctgct tcttgacgcc acctgtgaag ttctcaaagg
     3301 tggggtcagg cacgtactcg aaggccccgg cctctgttct gagcagggca cggtgcccgt
     3361 ccatctcgat cagcaccgtg aggttgtagg cctctggctc ctcaggcaca gccggggaca
     3421 ggaagacgac cttggtgtca ttgtggaaca cgtagtctgt acccaccacc gtcatgggct
     3481 gcagggattc agcctcccgc ggcggctgcc aggactgcag gggctccgcg atgaccacca
     3541 tggcaaacct ctggatcagg ctgaagccct gacccgtgac gttgatgctg cggccaccac
     3601 tggcaaagct tcgtagcggc tcgaaggctc gcagtacggg gttttcgcgg taggtgaaga
     3661 agatgccggg gttgggcacg ggggaccccc cgtaggagac ctccagaagc atctggcccc
     3721 gtgtcgcctg ggggccagtg acacactgga gctgcgcccc aaacttcgtc actttacacg
     3781 ggacgccgtt gagggtcacc cgcacgtcct cctgggagcc cgtgtccagg tgggtgccgt
     3841 ggatggtcag tgtggtgccg cccgcctgcg gtccctgctg cggctccaca ctgagaggct
     3901 tgggctgttg gaaggtgaac tggacattgg gaggcgaacg gcccagtttc ccgaagacgt
     3961 ccacctcgac accccccgtg aaaggcgtct ccgcagcctc gatcacacac acgatccggg
     4021 tggacacgga gtaacgttcc ggctgaaagg agcagttccg gccggccaca gagatcctct
     4081 ggatgtcccc tgcttggacg cccaaattgg accccaggat ggtgatgcgg atgcccccac
     4141 ccagggggcc cgtctcaggc tggatcctgg tgatgacggg cggcgggcac tcggaggtgg
     4201 tgttgcacag ggcctcatac acgcacctgc tctggccccc gcaccacgca cacctgtagt
     4261 cggggttagc ggcccggcac aggctgcagt cgctgcggcc aaaggagcag ttgtagaggg
     4321 tcacatggag cttgctgtcg atattcttgc cgtaagactt gacgtagagg tgcaggggca
     4381 gcgtctcgtt ggcatcgtgg gacagctttg gggtccgaaa ggcgaaggtc ccagattcct
     4441 gcatggtcac cggctccatg aacttgagca agtcactgcc cacgtgcagg gaggaaccct
     4501 tcacggtgtc caggttcttg ccctggaagt tcacatctgt ctcgtggttc atggggatca
     4561 ccagggggct gggtcccagg aactggggac agctgtcctc catgtgggca cggacgatgc
     4621 cgtcctcagg gttgggcgaa gcctcccggc actcgtggta gcgcaggtcc cactggcagg
     4681 tccagcggtt gctcacgcag gagatgcacg gcaggttctc ctccaggctc atggcctggc
     4741 ggcagtcgta gaaggggtac tggtaggacg tgaggaagat gttgcctcgt ctaaggagga
     4801 gctggatggt cacggccacg tggtcctggc ctggcggtgt gacggggatg ctgcttgggg
     4861 agttgcagat gacggcctcg ccctccacgc gggcggggtg tggcggcgac tccccaaaaa
     4921 ggcacagcaa ctcgtcctcc tcgctcaggg cagggagggg gctgacggtc agctgcacct
     4981 ccccctgggc ccgccggctc atgttctgtg gctgggcgct ggtgacggcc acgcaggact
     5041 tgcttcggct ccacagccag tggctggcct cctcggcccg cggacactcg gccttccggg
     5101 tgcatcgtcc ctcgacgacg caccagccgc agtaggggtc ctgggagtcg cggcactggg
     5161 tgcaggtcgg gtagctcagg cactcctgca ccggcagccg gaacaccttg tcctgggtca
     5221 tggcgtacag gctgcccagg tctccagaca gtaccaggtc gcgcttgact ctcttgttta
     5281 tctccacaag gatagagtcg tactctgagg aggtgccatc tggggtgagg tacaccttga
     5341 ggatccggcc atcagaggtg cccagaaaag caacagtgtg gttattttca gcagccacgg
     5401 tgacagccgt gaggttcagg cctccacgct gcagcacggc tgtgcctctg agcccgtcgc
     5461 ggctgcccag cgggtagggc aggtgctccg agccacatgg gaagctcttg ctggagcccg
     5521 gcgcgtggcc gccgcactgg atatcgccgt ggaagggctt gtagaagatg tcacgggcct
     5581 cccgggtgcc tgtgtaacag gcgttgcggt tggcctccat cttggcgtgc accttgtcca
     5641 gcgggaacag gcagaggccc gcaccgggcc ccccactgct ccggctgtct ctgctgaaga
     5701 cagcatatag caccctgcca gagccaggcg cagccacgga ggcggccagg caggtgccaa
     5761 aggcagcggc gtggatgtcg gggtcccggc actgcaggtc catctccagg taggagtagt
     5821 agttggggtc ttctctgcac atgcgtgcca gcagcgtgcg gttccgggcc gggtgcttgt
     5881 cctgctggtt gaagacaaag aagacgtagg ggccgtcctc gaaggccgcc acgaactgct
     5941 gtgtgttggt ggacaggtag ccggccttgt aggtggcgtg gtccgtgtag gcttcaaagg
     6001 cctccctgct gtcagtccgg tccaacagcc gagtgctcac gatgatgccg ttgtcgtgtg
     6061 gcccattgcc tttgcccaca aacagcacgc ggtcaccacc aggacccgtg gagctcacca
     6121 gccccactgt ggccacgccc tcatcattgc tggccacgaa agacttctcc ccgctgccgt
     6181 cctcgtagaa caggcggagg gagatgttgc tcagggcgcg cagagcgcag atgcccttga
     6241 agaggctgcc gcactccacc aggcgcttcc tgggagggtc gagcagcagc agctggttga
     6301 cattgtcagt catctcagcc tcatggcact ggctggcctc gatgggcggc gtgcacttct
     6361 tgttgtccag ggccgggccc gtggccacct gctgctccag ctgcagcttc gcatccagct
     6421 ggtagagggc attcaccgcc cccaggtaca ccacgcctga ggcctcatcc acagccaggt
     6481 ggttcagctc tttctcgctg cggaagaagt ccagcttgcg gggcctcagg ctggcacctg
     6541 cgcccagcag gcccagcagg gtcagggccc agagctgcag tgccatggtg ggccttgacg
     6601 gcccttctgc ttttttgtac aaacttgttg atatctgcag aattccacca cactggacta
     6661 gtcgacgaac cggtggatcc ccctggggag agaggtcggt gattcggtca acgagggagc
     6721 cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca
     6781 gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct
     6841 cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca
     6901 ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc
     6961 acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg
     7021 tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga
     7081 agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc
     7141 cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat
     7201 tcgatatcaa gcttctcgag gctagtctcg tgatcgataa aattttgaat ttttgtaatt
     7261 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     7321 gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg
     7381 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     7441 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     7501 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     7561 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     7621 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     7681 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     7741 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     7801 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     7861 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     7921 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     7981 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     8041 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     8101 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     8161 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     8221 ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
     8281 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     8341 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     8401 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
     8461 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     8521 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     8581 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     8641 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     8701 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     8761 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     8821 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     8881 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat
     8941 cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc
     9001 catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc
     9061 aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc
     9121 atgttgcaag actacaagag tattgcataa gactacatta agcttgcagc tccagctttt
     9181 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9241 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9301 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9361 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9421 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10381 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10441 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10501 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10561 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10621 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10681 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10741 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10801 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10861 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10921 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10981 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11041 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11101 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11161 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11221 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11281 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11341 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
    11401 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
    11461 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
    11521 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
    11581 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
    11641 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
    11701 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
    11761 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
    11821 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11881 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11941 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
    12001 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac
    12061 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
    12121 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
    12181 gttaggggcg ggatagctag agccagacat gataagatac attgatgagt ttggacaaac
    12241 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
    12301 atttgtaacc attataagct gcaataaaca agttcctctc actctctgat attcatttct
    12361 ttgcaagtta taaatactga ataataagat gacatgaact actactgcta gagattttcc
    12421 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
    12481 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
    12541 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
    12601 gcagatcttg tcttcgttgg gagtgagcta gcccttccag tccccccttt tcttttaaaa
    12661 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc gggccctcga
    12721 cggatcccct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
    12781 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
    12841 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
    12901 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
    12961 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
    13021 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
    13081 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
    13141 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
    13201 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
    13261 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
    13321 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
    13381 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
    13441 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
    13501 gtgcaatcca tcttgttcaa tggccgatcc catattggct gcaggtcgaa aggcccggag
    13561 atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc
    13621 ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccg
//
