LOCUS       sequence-171473-       13823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J,
            Magendantz M, Jacks T
  TITLE     The Nf2 tumor suppressor regulates cell-cell adhesion during tissue 
            fusion.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6.
  PUBMED    17360635
REFERENCE   2  (bases 1 to 13823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3261-6."
COMMENT     Sequence Label: pMB80 (R26-CreER)
FEATURES             Location/Qualifiers
     source          1..13823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    295..1088
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     CDS             1165..2193
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             2206..3141
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2254..3147
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    3208..3241
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3268..3767
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3807..4406
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3807..3826)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4303..4323
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    4953..4986
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4988..9328
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        9378..9877
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9904..10476
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10553..10618
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(10686..10703)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10692..10916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10978..10998)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10978..10996)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11017..11033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11017..11033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11030..11052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11041..11057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11065..11071,11072..11089,11090..11095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11110..11131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11248..11265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11419..12007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11499..11518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12178..12969,12970..13038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12801..12820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13039..13143)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13169..13624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13306..13327)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13518..13537
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13751..13773
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13765..13782
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13766..13782
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13792..13811
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13792..13810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccgctgg cgcgccttaa ttaaggctgg agccgttctg tgagacagcc gggtacgagt
       61 cgtgacgctg gaaggggcaa gcgggtggtg ggcaggaatg cggtccgccc tgcagcaacc
      121 ggagggggag ggagaaggga gcggaaaagt ctccaccgga cgcggccatg gctcgggggg
      181 gggggggcag cggaggagcg cttccggccg acgtctcgtc gctgattggc ttcttttcct
      241 cccgccgtgt gtgaaaacac aaatggcgtg ttttggttgg cgtaaggcgc ctgtcagtta
      301 acggcagccg gagtgcgcag ccgccggcag cctcgctctg cccactgggt ggggcgggag
      361 gtaggtgggg tgaggcgagc tggacgtgcg ggcgcggtcg gcctctggcg gggcgggggg
      421 ggggagggag ggtcagcgaa agtagctcgc gcgcgagcgg ccgcccaccc tccccttcct
      481 ctgggggagt cgttttaccc gccgccggcc gggcctcgtc gtctgattgg ctctcggggc
      541 ccagaaaact ggcccttgcc attggctcgt gttcgtgcaa gttgagtcca tccgccggcc
      601 agcgggggcg gcgaggaggc gctcccaggt tccggccctc ccctcggccc cgcgccgcag
      661 agtctggccg cgcgcccctg cgcaacgtgg caggaagcgc gcgctggggg cggggacggg
      721 cagtagggct gagcggctgc ggggcgggtg caagcacgtt tccgacttga gttgcctcaa
      781 gaggggcgtg ctgagccaga cctccatcgc gcactccggg gagtggaggg aaggagcgag
      841 ggctcagttg ggctgttttg gaggcaggaa gcacttgctc tcccaaagtc gctctgagtt
      901 gttatcagta agggagctgc agtggagtag gcggggagaa ggccgcaccc ttctccggag
      961 gggggagggg agtgttgcaa tacctttctg ggagttctct gctgcctcct ggcttctgag
     1021 gaccgccctg ggcctgggag aatcccttcc ccctcttccc tcgtgatctg caactccagt
     1081 ctttctagaa agggcgcagt agtccagggt ttccttgatg atgtcatact tatcctgtcc
     1141 cttttttttc cacagccgcg gaccatgtcc aatttactga ccgtacacca aaatttgcct
     1201 gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg
     1261 gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg
     1321 gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc
     1381 gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg
     1441 ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct
     1501 gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa
     1561 caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc
     1621 gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta
     1681 cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga
     1741 atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca
     1801 cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat
     1861 gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc
     1921 accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt
     1981 tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt
     2041 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct
     2101 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca
     2161 ggggcaatgg tgcgcctgct ggaagatggc gatctcgagc catctgctgg agacatgaga
     2221 gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc
     2281 ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc
     2341 tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc
     2401 aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt
     2461 gtggatttga ccctccatga tcaggtccac cttcttgaat gtgcctggct agagatcctg
     2521 atgattggtc tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac
     2581 ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg
     2641 ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc
     2701 ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag
     2761 tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc
     2821 cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc
     2881 ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc
     2941 atgaagtgca agaacgtggt gcccctctat gacctgctgc tggaggcggc ggacgcccac
     3001 cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac
     3061 ttggccactg cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag
     3121 gcagagggtt tccctgccac agcttgaacg cgtggcaata aaaagacaga ataaaacgca
     3181 cgggtgttgg gtcgtttgtt cgctagcata acttcgtata atgtatgcta tacgaagtta
     3241 tgaattcgtc gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     3301 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3361 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3421 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3481 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3541 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3601 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3661 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3721 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
     3781 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     3841 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     3901 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     3961 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4021 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4081 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4141 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4201 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4261 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4321 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4381 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     4441 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
     4501 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
     4561 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
     4621 gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata
     4681 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
     4741 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
     4801 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
     4861 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
     4921 tctaattcca tcagaagctg gtcgactcta gcataacttc gtataatgta tgctatacga
     4981 agttattcta gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt
     5041 gggcgttgtc ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca
     5101 gattttcggt tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag
     5161 gtagtcatct ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc
     5221 ggagtatttt ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc
     5281 ttgagatcct tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat
     5341 tttaatcatt tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc
     5401 ttatcaaaag gtattttaga acactcattt tagccccatt ttcatttatt atactggctt
     5461 atccaacccc tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac
     5521 tcatgaaacc agacagatta gttacataca ccacaaatcg aggctgtagc tggggcctca
     5581 acactgcagt tcttttataa ctccttagta cactttttgt tgatcctttg ccttgatcct
     5641 taattttcag tgtctatcac ctctcccgtc aggtggtgtt ccacatttgg gcctattctc
     5701 agtccaggga gttttacaac aatagatgta ttgagaatcc aacctaaagc ttaactttcc
     5761 actcccatga atgcctctct cctttttctc catttataaa ctgagctatt aaccattaat
     5821 ggtttccagg tggatgtctc ctcccccaat attacctgat gtatcttaca tattgccagg
     5881 ctgatatttt aagacattaa aaggtatatt tcattattga gccacatggt attgattact
     5941 gcttactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
     6001 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
     6061 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
     6121 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
     6181 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
     6241 gtgtgtgctt gtgctctata ataatactat ccaggggctg gagaggtggc tcggagttca
     6301 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
     6361 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
     6421 attcacatta aataaataaa tcctccttct tcttcttttt ttttttttta aagagaatac
     6481 tgtctccagt agaatttact gaagtaatga aatactttgt gtttgttcca atatggtagc
     6541 caataatcaa attactcttt aagcactgga aatgttacca aggaactaat ttttatttga
     6601 agtgtaactg tggacagagg agccataact gcagacttgt gggatacaga agaccaatgc
     6661 agactttaat gtcttttctc ttacactaag caataaagaa ataaaaattg aacttctagt
     6721 atcctatttg tttaaactgc tagctttact taacttttgt gcttcatcta tacaaagctg
     6781 aaagctaagt ctgcagccat tactaaacat gaaagcaagt aatgataatt ttggatttca
     6841 aaaatgtagg gccagagttt agccagccag tggtggtgct tgcctttatg cctttaatcc
     6901 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
     6961 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
     7021 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
     7081 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
     7141 gtcttttgac actgtgggct ttctttaaag cctccttcct gccatgtggt ctcttgtttg
     7201 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
     7261 atttgcagcc tgatttccag ggtggggttg ggaaatcttt caaacactaa aattgtcctt
     7321 taattttttt tttaaaaaat gggttatata ataaacctca taaaatagtt atgaggagtg
     7381 aggtggacta atattaaatg agtccctccc ctataaaaga gctattaagg ctttttgtct
     7441 tatacttaac ttttttttta aatgtggtat ctttagaacc aagggtctta gagttttagt
     7501 atacagaaac tgttgcatcg cttaatcaga ttttctagtt tcaaatccag agaatccaaa
     7561 ttcttcacag ccaaagtcaa attaagaatt tctgactttt aatgttaatt tgcttactgt
     7621 gaatataaaa atgatagctt ttcctgaggc agggtctcac tatgtatctc tgcctgatct
     7681 gcaacaagat atgtagacta aagttctgcc tgcttttgtc tcctgaatac taaggttaaa
     7741 atgtagtaat acttttggaa cttgcaggtc agattctttt ataggggaca cactaaggga
     7801 gcttgggtga tagttggtaa aatgtgtttc aagtgatgaa aacttgaatt attatcaccg
     7861 caacctactt tttaaaaaaa aaagccaggc ctgttagagc atgcttaagg gatccctagg
     7921 acttgctgag cacacaagag tagttacttg gcaggctcct ggtgagagca tatttcaaaa
     7981 aacaaggcag acaaccaaga aactacagtt aaggttacct gtctttaaac catctgcata
     8041 tacacaggga tattaaaata ttccaaataa tatttcattc aagttttccc ccatcaaatt
     8101 gggacatgga tttctccggt gaataggcag agttggaaac taaacaaatg ttggttttgt
     8161 gatttgtgaa attgttttca agtgatagtt aaagcccatg agatacagaa caaagctgct
     8221 atttcgaggt ctcttggttt atactcagaa gcacttcttt gggtttccct gcactatcct
     8281 gatcatgtgc taggcctacc ttaggctgat tgttgttcaa ataaacttaa gtttcctgtc
     8341 aggtgatgtc atatgatttc atatatcaag gcaaaacatg ttatatatgt taaacatttg
     8401 tacttaatgt gaaagttagg tctttgtggg tttgattttt aattttcaaa acctgagcta
     8461 aataagtcat ttttacatgt cttacatttg gtggaattgt ataattgtgg tttgcaggca
     8521 agactctctg acctagtaac cctacctata gagcactttg ctgggtcaca agtctaggag
     8581 tcaagcattt caccttgaag ttgagacgtt ttgttagtgt atactagttt atatgttgga
     8641 ggacatgttt atccagaaga tattcaggac tatttttgac tgggctaagg aattgattct
     8701 gattagcact gttagtgagc attgagtggc ctttaggctt gaattggagt cacttgtata
     8761 tctcaaataa tgctggcctt ttttaaaaag cccttgttct ttatcaccct gttttctaca
     8821 taatttttgt tcaaagaaat acttgtttgg atctcctttt gacaacaata gcatgttttc
     8881 aagccatatt ttttttcctt tttttttttt tttttggttt ttcgagacag ggtttctctg
     8941 tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat
     9001 ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt gcaccaccac gcctggctaa
     9061 gttggatatt ttgttatata actataacca atactaactc cactgggtgg atttttaatt
     9121 cagtcagtag tcttaagtgg tctttattgg cccttcatta aaatctactg ttcactctaa
     9181 cagaggctgt tggtactagt ggcacttaag caacttccta cggatatact agcagattaa
     9241 gggtcaggga tagaaactag tctagcgttt tgtataccta ccagctttat actaccttgt
     9301 tctgatagaa atatttcagg acatctagct tatcgatacc gtcgacggta tcgataagct
     9361 tgatatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     9421 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     9481 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     9541 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     9601 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     9661 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     9721 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     9781 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     9841 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat
     9901 cctgatgatg ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac
     9961 gggactaaac ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt
    10021 acacaaggaa attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac
    10081 gctgcgggat actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc
    10141 aaagtgacgt atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact
    10201 attaagaaag agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa
    10261 gagtttatca aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct
    10321 gaggggagtt ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta
    10381 gaacttgaga ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat
    10441 atggctcaag cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt
    10501 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
    10561 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
    10621 tccaacctat ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg
    10681 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
    10741 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
    10801 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
    10861 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
    10921 tgaggcggaa agaaccagct ggggctcgag ggggggcccg gtacccagct tttgttccct
    10981 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    11041 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11161 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11221 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11281 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11341 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11401 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11581 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11821 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    12001 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12061 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12121 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12181 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12241 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12301 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12361 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12421 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12481 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12541 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12601 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12661 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12721 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12781 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12841 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12901 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12961 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    13021 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13141 gcgcacattt ccccgaaaag tgcmayskaa attgtaagcg ttaatatttt gttaaaattc
    13201 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
    13261 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
    13321 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
    13381 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
    13441 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
    13501 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
    13561 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
    13621 gcgtcaggtg gcmcwttcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    13681 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    13741 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
    13801 tcactatagg gcgaattgga gct
//
