LOCUS       sequence-171474-        1201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PPM mislocaliser in P. falciparum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, 
            Jonscher E, Bergmann B, Spielmann T
  TITLE     A genetic system to study Plasmodium falciparum protein function.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
  PUBMED    28288121
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223."
COMMENT     Sequence Label: pLyn-FRB-mCherry-nmd3-BSD
FEATURES             Location/Qualifiers
     source          1..1201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             139..417
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             478..1188
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(623..641)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     891..910
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggatgta taaaatcaaa agggaaagac agcgcgggag cagatcccac cagaacgcgt
       61 gatccaacaa gaagtgcaaa tagtggagca ggagcaggag caggagcaat attaagtaga
      121 gctagcatgg cttctagaat cctctggcat gagatgtggc atgaaggcct ggaagaggca
      181 tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg
      241 catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat
      301 ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc
      361 aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaagact
      421 agtacaagtt atccatatga taatccagat tatgcaccag ttgcaacatt aggtaccatg
      481 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      541 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      601 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      661 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      721 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      781 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      841 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      901 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      961 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1021 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1081 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1141 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaacc cgggtcgagg
     1201 g
//
