LOCUS       sequence-171482-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN278
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGXDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3332..3356
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8188..8199
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8348..8372)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9833..9853)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9873..9889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9873..9889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9886..9908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagrcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctgrcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg cactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgggccc attaggtcag acaaaaattt ctgatcttta aaccttattg attaaaacta
     2341 atttttgccc gacttttttg acacatgcac tagacgcttg tactcaaaat ttggaaacct
     2401 tgctcaaatc cactactcag atatgacagt agcatcgata attgaaccat tatcctgaaa
     2461 tcccctttcc tgtcccggtt cattttccaa ttttttcaac ggtggaagaa ggggcaaatt
     2521 ttataccaaa acgtctccgt ttatgaccca ccccctatgc aaaataaacg gctctgggag
     2581 aaatagtaca gaaaacgcgg agcctgcttg attccttctc cgaggcgaca aaaaacgcat
     2641 cattcgtatt gtaagatgca tgtgtggctg gcctcttttg aattttactg gttttttata
     2701 tttctactaa tatttcgaaa aattttgtaa gtaaatcatt gattacgttt acaaaaattt
     2761 agaaacattt caaaatgatt acttctaaat taattatatt tacaggttag ttcgagttac
     2821 ttatgacctt ccaatccgcc atatccgcca aggacgctcc aattaatttt ttagaattat
     2881 tttttaacac atgtattatt ttttaacaat aaattattca tgaaatcatc aaatgtatat
     2941 gttctactat aatatttagg tcaaactata gaaaagaaat ccttgttttt ggaacaaagg
     3001 agttcagatc ctgtgcattt gatcttggat ccacatcttt ggtccatggg aaagcttggc
     3061 actgatcaag aaggattctt caaatatcta aaattgccgg ctgcccgtag aattccatca
     3121 gttttccaga gttttagtga aaatttgcat cccgttagaa acaatctaca caacggtaaa
     3181 aaggacccat tatttttacc gtaccgtttt cctttatggc cctctatgca aaaaattata
     3241 gcgtggaggg gcagtacaga ataataaagt gtgagaaaaa aaactgctct gcgagaaata
     3301 gtacagcaaa cgcggagcat gcttgattct atccactttg tacaagaaag ctgggttaat
     3361 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggagcc
     3421 ggctgtagca tgcggccgcg tagtaatcta gaaatgtaaa cctgtcattt ctgtgtattt
     3481 cagcaccgcg aagagcacca taaacagcta caacaagagc acgaacgtcg gctccaacag
     3541 aaaaagctgc aaaacgctgg tactcaccac atgctctgat aattgccatt tccttcgatt
     3601 tctgactttc aattgtgata tgataacctc aaaatctcca ttcaaaatga aaactcttaa
     3661 atttaagagc tttcaacacc ccgcactttc ccacctcatc gtttttccct tctatcttat
     3721 caattttttg tattttgtgt cagtttgcta tatcctcatc acctttttta ccgtttaatc
     3781 tctctatagt attgttcggg tgtttcaaga tgaaacgttt tgtgtgtagc atttttgaaa
     3841 cacagcgcga aaaatgcaga aactatcatc cacaatgcat taagtgcact cattgtgatg
     3901 actcctaacc ccctcgcccc accagttgtc tcttcacaaa ttcatggcac aattcaaaaa
     3961 tggctttttg tgttggatct tgatctcgtc tctttccttc ttctttcgtt tctctcggac
     4021 cactcattat tcgaatgcca tgcatttgtt gatgatgcgc agcgatggtc gcacacacta
     4081 caacagatat gatggtggct gtgcgtggtg cagagctcat gaataagtga tggggcaccg
     4141 agatgtgact cctcccatta ttcgtcttga gtcgcatctt ttgtcttggt ccgttgtcag
     4201 tcgagcctca aggcagccgt tccgccagtg atcgcctgct aagaagttgt aggtgtagag
     4261 gaagaaacgt cgggtccaaa tttcaagcaa cggtaccaaa aatgccacaa ttcgacattc
     4321 tctgtaagac accaccaaaa gtcctcgtcc gtcaattcgt cgagcgtttc gagcgtccat
     4381 ccggagagaa gatcgccctc tgcgccgccg agctcaccta cctctgctgg atgatcaccc
     4441 acaacggaac cgccatcaag cgtgccacct tcatgtccta caacaccatc atctccaact
     4501 ccctctcctt cgacatcgtc aacaagtccc tccaattcaa gtacaagacc caaaaggcca
     4561 ccatcctcga ggcctccctc aaggtaagtt taaacatata tatactaact aaccctgatt
     4621 atttaaattt tcagaagctc atcccagcct gggagttcac catcatccca tactacggac
     4681 aaaagcacca atccgacatc accgacatcg tctcctccct ccaactccaa ttcgagtcct
     4741 ccgaggaggc cgacaaggga aactcccact ccaagaagat gctcaaggcc ctcctctccg
     4801 agggagagtc catctgggag atcaccgaga agatcctcaa ctccttcgag tacacctccc
     4861 gtttcaccaa gaccaagacc ctctaccaat tcctcttcct cgccaccttc atcaactgcg
     4921 gacgtttctc cgacatcaag aacgtcgacc caaagtcctt caagctcgtc caaaacaagt
     4981 acctcggagt catcatccaa tgcctcgtca ccgagaccaa gacctccgtc tcccgtcaca
     5041 tctacttctt ctccgcccgt ggacgtatcg acccactcgt ctacctcgac gagttcctcc
     5101 gtaactccga gccagtcctc aagcgtgtca accgtaccgg aaactcctcc tccaacaagc
     5161 aagagtacca actcctcaag gacaacctcg tccgttccta caacaaggcc ctcaagaaga
     5221 acgccccata ctccatcttc gccatcaaga acggaccaaa gtcccacatc ggacgtcacc
     5281 tcatgacctc cttcctctcc atgaaggtaa gtttaaacat gattttacta actaactaat
     5341 ctgatttaaa ttttcaggga ctcaccgagc tcaccaacgt cgtcggaaac tggtccgaca
     5401 agcgtgcctc cgccgtcgcc cgtaccacct acacccacca aatcaccgcc atcccagacc
     5461 actacttcgc cctcgtctcc cgttactacg cctacgaccc aatctccaag gagatgatcg
     5521 ccctcaagga cgagaccaac ccaatcgagg agtggcaaca catcgagcaa ctcaagggat
     5581 ccgccgaggg atccatccgt tacccagcct ggaacggaat catctcccaa gaggtcctcg
     5641 actacctctc ctcctacatc aaccgtcgta tctaacgcgt atgtatctgc atatccttca
     5701 aatgttgtcc atattgtatc agtaaattat aatgccccct ttttatatat tctccttatc
     5761 atgtaaatgt tctattgatt ttttgctggt gtaaaacgtt tttatagtaa atcctctcca
     5821 cagtgaattt gttaaattgt atcaaaacaa gacattgatt aaatttgttc gagacttgtg
     5881 gattgtaaaa tattttgcag aaaatggcaa tattcattta gaaacattac ttccataata
     5941 ggaaaaacta tttatgagtt ttcatgtgcc tatgcaagtt cccactctgt gtgaatttca
     6001 tcatacaaat tgagcaaaca taaggtttat gtccagtatg ggttctcaga tgttttgtaa
     6061 gagttgagct gtcggtgaat gccttctcac attgagcaca tggatacggt ctctctccag
     6121 tgtgtgttct gaaataatga aaaatagtct tgcaatatgt ttctactatt cgagaaaaaa
     6181 tgtaattttt tttcttatgg taacccggct ttccccgggc ctagttctag acattctcta
     6241 atgaaaaaat ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac
     6301 agatttccgt gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa
     6361 caagggggga actgaaaagc ttaggaatgc attgaacatg agaaggggaa ggggaaggaa
     6421 caaactagac aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag
     6481 aataattatc cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt
     6541 gttgtgcatg acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg
     6601 attgttgatc atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa
     6661 taatgactca ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt
     6721 tcgtgaattc ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt
     6781 gaagaagtga cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc
     6841 cacagttgct ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta
     6901 tcttgccgac tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc
     6961 gaaaagcact tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata
     7021 gatgttcgca gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag
     7081 ggagcaagat accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag
     7141 ctttcttcgc aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg
     7201 gcatctggaa atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat
     7261 caagagttag ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc
     7321 cgagccgggt ggaatcgatc gttgttgctc ggctttcata tcggtttggt tggaagcggc
     7381 tgaaaacgga aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta
     7441 gagggtgcca aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa
     7501 atacgataat gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt
     7561 gagtacatgt aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt
     7621 ttctatttat aacgctaata atttgaaaaa gaaacctatt gcgaaccgcg gggtgaatcc
     7681 caaaaacgaa tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga
     7741 cgtggaatag agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa
     7801 tgaatgaggg tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga
     7861 agattgagaa gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg
     7921 aacgagcgga aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac
     7981 actatccaag aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata
     8041 ggaacagaat aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt
     8101 aacttaattt gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat
     8161 aaccggtttg ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat
     8221 cccggtttct gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata
     8281 ataaacattt tatccgttaa caattttcga atacttttta caaggacttg gataaattgg
     8341 ctcaaagagc ctgctttttt gtacaaactt gaattcaaaa acgcagaact cgtactgttg
     8401 gatgcctgtg tagcggtcct ctattgattt ttgctatttt ttgataattt tactgtaaat
     8461 tatgagttat tccaattaac attcctagct aaatgtaagt tagcgaccaa ttttttagca
     8521 accccatttt atgacttttc agaatatcgc ctactgggtc aacgacactt gtttggaact
     8581 aagggtcttc ccaatttttt ttaaatcttc ataggcttga cagaatcagg gttcttcaag
     8641 cagctttgag agggaatcaa ttgcaacatt tgatgttaaa tttatatttt ttatagttct
     8701 ctggtgaaat ggggaccacc gcaaagcccc ttttatgttt ttaacttgaa taaaatcttg
     8761 tattaaattg tgatttatag atctgtatgg gtaatttgaa ggaaaagaaa gggagctccc
     8821 aaaagtgcct tacagatcca ggcccaataa atgtaaacta ttgggcagta gtatggcaaa
     8881 aatcgtgcgg aatttgaaaa acctagaatt aaaataaata ttcaacacaa atgttcgaga
     8941 tattttaagt tcggagtgta tatctagaac atattgtaca agtaggtcta cgggatcgtc
     9001 tacgggaaaa tttgatttcc tcgaagatgt atttaatgaa ttctctaatg ttgcaggtct
     9061 ttgtatttga cagaatgttg gtttttgaaa cttattcgcc ataattgctt ggctactaca
     9121 tgtctcatgc cattgacact atcctgtact cgaaatttgt taagtaacta cccaccgtcc
     9181 acttatgacg agagggtcaa ttaaggtcac tccacaaaag taaatttgaa aacacatgag
     9241 aaaaagtgat ctgaattccc ttgaagtggc taaaacaaag cagatgaggg atacgacgta
     9301 tgcgactact gagagataag gtcgaaactt gataaggatg ctgcttacct gcttagcaag
     9361 tttcttcgca caggagaaag tgacaagcta aactatcaaa ttcatctaaa acctcaaaaa
     9421 attattttgg atgaaataat gttttatttt gagcaacgca taaggcaaag aataaattaa
     9481 aggcgatccg cttagcggcg agttccgtcc tcgaagaaga ttcctccatc gatggcgcag
     9541 tactttggac agattccacg ctctcctggg gttcctggtg gtccgactgg tcctggctgt
     9601 cctgggtttc cgtcggctcc tggcttgccg tctggtcctg gtggtccctt tggtccaggt
     9661 tgtcctgaaa gatgtggtga ttagatatgg attgaaaaac tatgaaatga ttacctgggg
     9721 ttccgtcgtt tcctggttgt ccggatggtc ctggtggtcc gactggtcct ggctctccgg
     9781 tggatcctgg ctctcccttt tggactggct cgcagacagc tggaagtccc tccaactttt
     9841 ctatacaaag ttgatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9901 tatccgctgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9961 aacgcaggaa agaacatgtg agcaaaaggc ca
//
