LOCUS       sequence-171499-       12165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai 
            YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, 
            Keasling JD, Deutsch S, Loque D
  TITLE     Exploiting members of the BAHD acyltransferase family to synthesize 
            multiple hydroxycinnamate and benzoate conjugates in yeast.
  JOURNAL   Microb Cell Fact. 2016 Nov 21;15(1):198.
  PUBMED    27871334
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2016-11-21"; volume: "15(1)"; pages: "198"
COMMENT     Sequence Label: pDRf1-4CL5-DsRed (JBEI-15461)
FEATURES             Location/Qualifiers
     source          1..12165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    60..78
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             121..132
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             139..816
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      834..905
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      921..948
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    complement(963..987)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1049..1068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1050..1068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1216..1403
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1271..1295)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      3557..3804
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3573..3591)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3816..3833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3816..3832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3825..3847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4268..5614
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             6568..7662
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8441..8656)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8681..8699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8767..8871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8872..8940,8941..9732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9090..9109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9903..10491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10392..10411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10645..10662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10779..10800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10815..10820,10821..10838,10839..10845)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10853..10869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10858..10880
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10877..10893
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10877..10893
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(10923..11378)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11060..11081)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11272..11291
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actagtgaat tcgcggccat cacaagtttg tacaaaaaag caggcttcca cgtggatctt
       61 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacggatctg
      121 aaagaggaga aaggatctat ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc
      181 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      241 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      301 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      361 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      421 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      481 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      541 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      601 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      661 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      721 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      781 gaacgtgctg aaggtcgtca ctccaccggt gcttaataag gatctccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatagcacgt
      961 ggacccagct ttcttgtaca aagtggtgta gttaattcat gatggccgct gcaggtcgac
     1021 ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcggatcca
     1081 gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct
     1141 accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt
     1201 gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
     1261 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg anaattctta
     1321 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
     1381 cttattgacc acacctctac cggcatgccg agctcgtagg aacaatttcg ggcccctgcg
     1441 tgttcttctg aggttcatct tttacatttg cttctgctgg ataattttca gaggcaacaa
     1501 ggaaaaatta gatggcaaaa agtcgtcttt caaggaaaaa tccccaccat ctttcgagat
     1561 cccctgtaac ttattggcaa ctgaaagaat gaaaaggagg aaaatacaaa atatactaga
     1621 actgaaaaaa aaaaagtata aatagagacg atatatgcca atacttcaca atgttcgaat
     1681 ctattcttca tttgcagcta ttgtaaaata ataaaacatc aagaacaaac aagctcaact
     1741 tgtcttttct aagaacaaag aataaacaca aaaacaaaaa gtttttttaa ttttaatcaa
     1801 aaagttctag tggatccccc gggcggccgc atggtgctcc aacaacaaac gcattttctc
     1861 acaaagaaga tagatcaaga agacgaagaa gaagaacctt ctcatgattt cattttccga
     1921 tctaagcttc ctgatatctt catccctaac cacctccctc tcaccgatta cgtcttccaa
     1981 aggttctccg gcgatggcga cggagattcc tcaacaacct gtatcatcga cggtgccact
     2041 ggccgtattt tgacctacgc cgatgtacaa accaacatgc ggaggatagc tgccggaatc
     2101 caccggctcg ggatccgcca cggagacgtc gtaatgcttc tcctcccgaa ctcgccggag
     2161 tttgctctgt cttttcttgc cgttgcttac cttggagccg tttccactac cgcgaatccg
     2221 ttctacactc agccggagat cgctaaacag gctaaagcct ccgccgcgaa gatgatcatc
     2281 acgaagaaat gtttagtcga taaactaaca aacctcaaaa acgacggcgt tttgatcgtt
     2341 tgcctagacg atgacggtga caacggcgtt gtttcgtcga gtgatgatgg ttgcgtgagt
     2401 ttcacggaac tgactcaagc ggacgaaaca gaacttctaa aacctaaaat atcgccggaa
     2461 gacacggtgg cgatgccgta ctcttccgga acaacgggac ttccaaaggg agtgatgatt
     2521 actcacaagg gtttagttac aagcatcgct cagaaagtcg acggagaaaa ccctaacctt
     2581 aacttcacgg ccaatgacgt catcctctgt tttctcccaa tgtttcacat ttacgctctc
     2641 gacgcgttga tgctttcggc gatgagaacc ggtgcagcgt tactgatcgt gccgaggttc
     2701 gagttgaatc tggtgatgga gctgattcaa aggtacaaag tcacggttgt gccggtggct
     2761 cctccggtgg ttttagcatt cattaaatcc cctgagacgg agagatacga cttgagctcg
     2821 gtgagaatta tgctatcagg tgcagctaca ctaaagaagg agcttgaaga cgccgtgcga
     2881 ctcaagtttc ctaatgccat ttttggtcag ggttatggaa tgaccgagtc cggaacggta
     2941 gctaagtcat tagcgtttgc aaagaaccca tttaaaacca agtccggtgc atgtgggact
     3001 gtgatcagaa acgccgagat gaaagtggtc gataccgaaa ccggaatctc cttgccgcgc
     3061 aacaaatctg gcgagatctg cgtccgaggt catcagctca tgaaaggtta tttgaatgat
     3121 ccggaagcta ctgcacgaac catagacaaa gacgggtggt tacatacagg agatattggg
     3181 tttgtggatg atgatgatga gatcttcatt gttgatcggt tgaaggaact gatcaaattc
     3241 aaaggctacc aagtggctcc agctgagctt gaagcattgc ttatttctca tccttctatc
     3301 gatgatgccg cagttgttgc aatgaaggat gaagtagctg atgaggttcc agtagcattt
     3361 gtagcaagat ctcaagggtc tcagttaact gaagatgatg tcaagagtta cgtaaacaaa
     3421 caggtggttc actacaagcg gattaagatg gtgtttttca tagaagttat accaaaagca
     3481 gtttcgggca agattctgag gaaggatctg cgagctaaat tggaaaccat gtgctctaaa
     3541 taggcggccg ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
     3601 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     3661 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
     3721 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
     3781 ggacgctcga aggctttaat ttgcgcatgc aattcactgg ccgtcgtttt acaacgtcgt
     3841 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3901 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3961 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4021 cgcataatcg gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt
     4081 tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac
     4141 ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat
     4201 cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat
     4261 ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     4321 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     4381 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     4441 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     4501 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     4561 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     4621 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     4681 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     4741 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     4801 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     4861 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     4921 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     4981 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     5041 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     5101 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     5161 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     5221 aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact
     5281 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     5341 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     5401 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     5461 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     5521 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     5581 ccttcaatgc tatcatttcc tttgatattg gatcgatccg atgataagct gtcaaacatg
     5641 agaattgggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
     5701 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
     5761 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag
     5821 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata
     5881 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca
     5941 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc
     6001 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc
     6061 cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc
     6121 cgcctgcttc aaaccgctaa caatacctgg gcccgtggga atactcaggt atcgtaagat
     6181 gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca
     6241 ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt
     6301 cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagaggc
     6361 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat
     6421 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat
     6481 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatttcaa
     6541 ggatatacca ttctaatgtc tgcccctatg tctgccccta agaagatcgt cgttttgcca
     6601 ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct
     6661 gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc
     6721 gatgctacag gtgtcccact tccagatgag gcgctggaag cctccaagaa ggttgatgcc
     6781 gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa
     6841 ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt
     6901 gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac
     6961 ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat
     7021 ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca
     7081 agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat
     7141 aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag
     7201 aacgaatttc ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta
     7261 gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc
     7321 atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg
     7381 gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct
     7441 ccagatttgc caaagaataa ggttgaccct atcgccacta tcttgtctgc tgcaatgatg
     7501 ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag
     7561 gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc
     7621 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     7681 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     7741 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     7801 aaggagaaaa aggaggatag taaaggaata caggtaagca aattgatact aatggctcaa
     7861 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     7921 caaaaagtta ggtgtaacag aaaatcatga aactacgatt cctaatttga tattggagga
     7981 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     8041 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     8101 attttactct gtcagaaacg gccttaacga cgtagtcgac caattccgat catatcaata
     8161 acccttaata taacttcgta taatgtatgc tatcgaagtt attaggtctg aagaggagtt
     8221 tacgtccagc catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt
     8281 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
     8341 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
     8401 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc
     8461 atgtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
     8521 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
     8581 cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa
     8641 aaaatgatga attgaaaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     8701 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8761 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8821 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8881 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8941 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9001 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9061 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9121 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9181 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9241 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9301 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9361 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9421 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9481 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9541 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9601 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9661 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9721 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9781 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9841 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9901 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9961 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10021 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10081 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10141 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10201 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10261 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10321 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10381 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10441 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10501 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10561 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10621 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    10681 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    10741 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10801 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10861 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct taccgcatca
    10921 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    10981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
    11041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    11101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    11161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    11221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    11281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    11341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccaag cttcctgaaa
    11401 cggagaaaca taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc
    11461 ttttccggta atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc
    11521 agaaataaac ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg
    11581 agaatttcgt aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct
    11641 cgatcgagac cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc
    11701 tgaagaagct atagtagaac tatgagcttt ttttgtttct gttttccttt tttttttttt
    11761 tacctctgtg gaaattgtta ctctcacact ctttagttcg tttgtttgtt ttgtttattc
    11821 caattatgac cggtgacgaa acgtggtcga tggtgggtac cgcttatgct cccctccatt
    11881 agtttcgatt atataaaaag gccaaatatt gtattatttt caaatgtcct atcattatcg
    11941 tctaacatct aatttctctt aaattttttc tctttctttc ctataacacc aatagtgaaa
    12001 atcttttttt cttctatatc tacaaaaact ttttttttct atcaacctcg ttgataaatt
    12061 ttttctttaa caatcgttaa taattaatta attggaaaat aaccattttt tctctctttt
    12121 atacacacat tcaaaagaaa gaaaaaaaat ataccccagc ctcga
//
